Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Dan Ferullo Abstract 1-29-04 The role for CheY in the rotational direction of the flagellar motor has been further characterized (doesn’t say very much-could you leave this sentence out?). Previous studies have proposed a model in which ligand release or synthesis of a molecule is necessary for CheY to influence flagella. Our findings suggest a new model in which the clockwise rotation of flagella does not require the involvement of either the chemotactic components of the methylation system or the presence of the major chemoattractant receptors. We have devised a system in which production of CheY through the use of a promoter on an expression plasmid construct allows for levels of transcription in Escherichia Coli to be adjustable. Revise sentence Various expression levels of cheY were observed in both wild type and mutant cells that contained a deletion plasmid ??? for other chemotactic gene products. Specifically, overproduction and endogenous levels of CheY in both wild type and mutant cells exhibited either a shift from a counter-clockwise rotation to a clockwise rotation. This, in effect, corresponds to an increase in tumbling frequency in free-swimming bacteria. The results suggest that CheY interacts with a detector on the flagellar motor, potentially through another chemotaxis-specific gene product, to promote a clockwise rotation in flagella. (There is a jump in the sequence of logic from the deletion of the other genes in the che pathway to this last statement. You need to fill in the missing step.)