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Name _______________ Period _____ What are the differences between plant and animal cells? Objectives: Students will prepare and view slides of different cell types. Students will sketch the cell structures and describe, in their own terms, the differences and similarities between different cells. Materials: Microscope Scalpel Pipettes Water Slides and cover slips Prepared slides Methylene blue Iodine Elodea Potato Human cheek cells Red Bell Pepper Green bell pepper Superbena Leaf Other slide material Caution with the scalpels and stains!!! Procedure: 1. Remove a piece of the thin layer of transparent epidermal tissue from the inner surface of a piece of onion. a. Place a drop of water on the slide b. Then place a small (Unwrinkled, hopefully) piece of the epidermal tissue in the water. c. Cover it with a cover slip d. Examine the onion tissue under low power, and under high power e. Remove the slide from the microscope f. Place a drop of iodine next to one edge of the cover slip and touch a piece of paper towel to the opposite edge. The stain will be drawn under the cover slip and into the tissue. g. Re-examine the onion tissue under low, medium, and high power. h. Draw a few cells, and label the cell wall, nucleus, cytoplasm, and cell membrane. i. Wash your slide and use it for the next observation. 2. Slice a very thin piece of potato. Prepare the slide, with iodine stain, as described above. Draw a few of the cells and label the cell wall and leucoplasts. (Iodine changes color from brown to blue or black when it comes in contact with starch, and the leucoplasts are the blue/black parts inside each potato cell). 3. Make a slide of a cheek cell, staining it with methylene blue, as you did in the previous lab. Under high power sketch a few cells and label the cell membrane, nucleus, and cytoplasm. 4. Remove a single leaf from the Elodea plant. Place the leaf on a slide and add a drop of water and the cover slip. Observe the leaf under low and medium power. Sketch it under medium and label the cell wall, cell membrane, chloroplasts, vacuole, and nucleus (if they’re visible). 5. Make a thin slice of a colored pepper. Prepare a slide and observe. Sketch cells (under at least medium power) and label cell walls, and chromoplasts (Chloroplasts are green in green plants, so chromoplasts in different colored plants must be…). 6. Place a drop of pond water on a slide. Examine the slide under low and medium power looking for microorganisms. Observe, draw and label at least one. 7. Slice off a piece of the Superbena or other plant leaf and try to peel off the bottom lasyer. Observe the cells on at least medium power. Sketch and label the cells. 8. Choose a prepared slide. Observe on medium power, draw & label. 9. Sketch and label (On at least medium power) other cells in order to finish all the sketches, and fill all the boxes. 10. Clean up and return microscopes. Questions: 1. Name at least three ways in which Elodea and cheek cells differ. a. b. c. 2. How do cheek cells and blood cells differ (other than color)? 3. What is stored in the leucoplasts of potato cells? How do you know? 4. What was in the chromoplasts of the pepper cells? 5. How many different types of cells did you find on the underside of the leaf? Describe each. 6. Describe three differences between plant and animal cells. a. b. c. Drawings Cheek Cell Onion Cell ____X Blood Cell Pond Water _____X Potato Cell _____X Elodea Leaf _____X Superbena _____X Colored Pepper _____X _____X _____X Other: _____X