Protein S deficiency
... a membrane composition which has little, if any, effect on the procoagulant reactions. PE and oxidation increase the binding of many antiphospholipid antibodies and the ability of at least some of them to inhibit the APCprotein S complex specifically. IgG purified from several antiphospholipid syndr ...
... a membrane composition which has little, if any, effect on the procoagulant reactions. PE and oxidation increase the binding of many antiphospholipid antibodies and the ability of at least some of them to inhibit the APCprotein S complex specifically. IgG purified from several antiphospholipid syndr ...
P6039Datasheet-Lot0041305
... to the serine or threonine. The Enterococcus faecalis O-Glycosidase, also called Endo-α-NAcetylgalactosaminidase, can then remove these core structures with no modification of the serine or threonine residues. Any modification of the core structures, including sialyation, will block the action of th ...
... to the serine or threonine. The Enterococcus faecalis O-Glycosidase, also called Endo-α-NAcetylgalactosaminidase, can then remove these core structures with no modification of the serine or threonine residues. Any modification of the core structures, including sialyation, will block the action of th ...
CHAPTER 22
... Discuss the basis for secondary structure prediction in proteins. How reliable is it? Answer: The basis for secondary structure prediction is that certain amino acids tend to be found more frequently in helices or β sheets. This information is derived from the statistical frequency of amino acids ...
... Discuss the basis for secondary structure prediction in proteins. How reliable is it? Answer: The basis for secondary structure prediction is that certain amino acids tend to be found more frequently in helices or β sheets. This information is derived from the statistical frequency of amino acids ...
Peroxisomes - University of California San Diego
... species, but not in any metazoan. A ,70 kDa, predominantly cytoplasmic/partly peroxisomal protein that is found in yeasts, plants and humans. Contains a PTS1binding, tetratricopeptide-repeat (TPR) domain in its carboxy-terminal half, interacts with several peroxins (Pex7, Pex8, Pex10, Pex12, Pex13 a ...
... species, but not in any metazoan. A ,70 kDa, predominantly cytoplasmic/partly peroxisomal protein that is found in yeasts, plants and humans. Contains a PTS1binding, tetratricopeptide-repeat (TPR) domain in its carboxy-terminal half, interacts with several peroxins (Pex7, Pex8, Pex10, Pex12, Pex13 a ...
Proti-Ace Kit - Hampton Research
... 4. Pipette 10 ml of the 1:100 protease stock into 10 ml aliquots of protein (10 mg/ml) for each protease to be screened. 5. Incubate at 37°C for 60 minutes. 6. Stop the reaction by adding SDS-PAGE sample buffer for SDS-PAGE analysis or a final concentration of 10% v/v trichloroacetic acid for MS ana ...
... 4. Pipette 10 ml of the 1:100 protease stock into 10 ml aliquots of protein (10 mg/ml) for each protease to be screened. 5. Incubate at 37°C for 60 minutes. 6. Stop the reaction by adding SDS-PAGE sample buffer for SDS-PAGE analysis or a final concentration of 10% v/v trichloroacetic acid for MS ana ...
Ribosome-tethered molecular chaperones
... addition, non-ribosome-bound chaperones act on longer nascent chains, either during the process of translation, or after they have been released from the ribosome. Although chaperones carrying out these functions might be structurally unrelated, like all chaperones they share the ability to interact ...
... addition, non-ribosome-bound chaperones act on longer nascent chains, either during the process of translation, or after they have been released from the ribosome. Although chaperones carrying out these functions might be structurally unrelated, like all chaperones they share the ability to interact ...
In Depth Analysis of the Spectra Unassigned by Database Search
... Figure 4. The peptide-spectrum annotation reports strong evidence peaks for the mutation at site. We have not tried to interpret all the de novo only peptides. Potentially, these peptides can be from endogenous peptides, cross-linked peptides, contaminants, or peptides with more complex PTMs such as ...
... Figure 4. The peptide-spectrum annotation reports strong evidence peaks for the mutation at site. We have not tried to interpret all the de novo only peptides. Potentially, these peptides can be from endogenous peptides, cross-linked peptides, contaminants, or peptides with more complex PTMs such as ...
hal.archives-ouvertes.fr
... the Arabidopsis VSRs had been isolated from CCVs delivering proteins to the lytic vacuole, the involvement of such receptors in the sorting of storage proteins was discarded. The diversity of species and tissues used in those studies made difficult the interpretation of the results obtained so far. ...
... the Arabidopsis VSRs had been isolated from CCVs delivering proteins to the lytic vacuole, the involvement of such receptors in the sorting of storage proteins was discarded. The diversity of species and tissues used in those studies made difficult the interpretation of the results obtained so far. ...
as PDF
... characteristics indicate the existence of two distinct receptor binding-sites within the globular domain of adiponectin, each capable of interacting with either AdipoR1 or AdipoR2. The finding that the AdipoR1 binding-site in full-length adiponectin is inaccessible for receptor binding suggests that ...
... characteristics indicate the existence of two distinct receptor binding-sites within the globular domain of adiponectin, each capable of interacting with either AdipoR1 or AdipoR2. The finding that the AdipoR1 binding-site in full-length adiponectin is inaccessible for receptor binding suggests that ...
Lipid–protein interactions probed by electron crystallography
... head group from this lipid in turn forms a salt bridge with the side chain amine from lysine 40 on bR. At the threefold axis of bR, three PM lipids were observed to plug the central cavity on the extracellular side. The head groups of these three central lipids are vertically lower by 5 Å than the ...
... head group from this lipid in turn forms a salt bridge with the side chain amine from lysine 40 on bR. At the threefold axis of bR, three PM lipids were observed to plug the central cavity on the extracellular side. The head groups of these three central lipids are vertically lower by 5 Å than the ...
sv-lncs - School of Mathematical and Computer Sciences
... from metabolic,[5] to protein networks,[6, 7] strongly support the proposal that modular architecture is one of the principles underlying biological organisation. The modular nature of the cellular networks, including PPI networks, is reflected by high clustering, measured by clustering coefficient. ...
... from metabolic,[5] to protein networks,[6, 7] strongly support the proposal that modular architecture is one of the principles underlying biological organisation. The modular nature of the cellular networks, including PPI networks, is reflected by high clustering, measured by clustering coefficient. ...
Application of a bacterial two-hybrid system for the
... peak sizes of the recombinant proteins could be determined. For the monomers of FmhB, FemA and FemB, the measured molecular masses correlated well with the theoretical values derived from the amino acid sequences, and it was determined that FemA and FemB formed dimers (Fig. 3a, b; Table 3). GST-pull ...
... peak sizes of the recombinant proteins could be determined. For the monomers of FmhB, FemA and FemB, the measured molecular masses correlated well with the theoretical values derived from the amino acid sequences, and it was determined that FemA and FemB formed dimers (Fig. 3a, b; Table 3). GST-pull ...
Assembly and function of cell surface structures of the
... et al., 2007, Sleytr et al., 2007). The arrays exhibit six-, four-, three-, or twofold symmetries depending on the species (summarized in (Engelhardt & Peters, 1998, Engelhardt, 2007, Konig et al., 2007). One or two (glyco-) protein subunits of molecular masses ranging from 40 to 210 kDa usually mak ...
... et al., 2007, Sleytr et al., 2007). The arrays exhibit six-, four-, three-, or twofold symmetries depending on the species (summarized in (Engelhardt & Peters, 1998, Engelhardt, 2007, Konig et al., 2007). One or two (glyco-) protein subunits of molecular masses ranging from 40 to 210 kDa usually mak ...
Protein structure is conceptually divided into four
... sequence) is connected to strand n + 3 (a) or n - 3 (b) instead of n + 1 or n - 1 in an eight-stranded antiparallel b sheet or barrel. The two different possible connections give two different hands of the Greek key motif. In all protein structures known so far, the hand shown in (a) has been mostly ...
... sequence) is connected to strand n + 3 (a) or n - 3 (b) instead of n + 1 or n - 1 in an eight-stranded antiparallel b sheet or barrel. The two different possible connections give two different hands of the Greek key motif. In all protein structures known so far, the hand shown in (a) has been mostly ...
A toolbox for validation of mass spectrometry peptides identification
... assigned to each peptide spectrum matches (PSMs). For each query, PSMs are ranked according to their score. Mascot then groups PSMs into protein hits. A hit contains not only all proteins covered by the same set of PSMs but also all proteins covered by a subset of these PSMs. A hit score, based on ...
... assigned to each peptide spectrum matches (PSMs). For each query, PSMs are ranked according to their score. Mascot then groups PSMs into protein hits. A hit contains not only all proteins covered by the same set of PSMs but also all proteins covered by a subset of these PSMs. A hit score, based on ...
Protein aggregation in bacteria: the thin boundary
... proteome is both stable and functional. It implicates an equilibrium between synthesis, folding, trafficking, aggregation, disaggregation and degradation. In accordance with the eukaryotic systems, it has been documented that protein aggregation also reduces fitness of bacterial cells, but although ...
... proteome is both stable and functional. It implicates an equilibrium between synthesis, folding, trafficking, aggregation, disaggregation and degradation. In accordance with the eukaryotic systems, it has been documented that protein aggregation also reduces fitness of bacterial cells, but although ...
Full Text
... understanding of interactions in protein structures. Therefore, we have been interested in developing a representation for biological sequences that can incorporate structural features conferred through dependences among amino acids. We have used Bayesian networks (Neapolitan, 1990; Pearl, 1988) to ...
... understanding of interactions in protein structures. Therefore, we have been interested in developing a representation for biological sequences that can incorporate structural features conferred through dependences among amino acids. We have used Bayesian networks (Neapolitan, 1990; Pearl, 1988) to ...
Sample pages 2 PDF
... To make a long story short, the structure and properties of the resulting protein synthesized with all D or L amino acids differ. The L form is predominantly found in proteins of higher life species and can be deciphered from the D form by the position of the R group with respect to the backbone. In ...
... To make a long story short, the structure and properties of the resulting protein synthesized with all D or L amino acids differ. The L form is predominantly found in proteins of higher life species and can be deciphered from the D form by the position of the R group with respect to the backbone. In ...
PDF + SI - Journal of Cell Science
... Intracellular trafficking and protein sorting are mediated by various protein complexes, with the retromer complex being primarily involved in retrograde traffic from the endosome or lysosome to the Golgi complex. Here, comparative genomics, cell biology and phylogenetics were used to probe the earl ...
... Intracellular trafficking and protein sorting are mediated by various protein complexes, with the retromer complex being primarily involved in retrograde traffic from the endosome or lysosome to the Golgi complex. Here, comparative genomics, cell biology and phylogenetics were used to probe the earl ...
Pdf
... the 20 amino acids could be represented by a 5-bit binary number ranging from 00001 to 11111. To reflect a physically relevant property, such as hydrophobicity for example, the amino acids would be assigned a 5-bit number depending on where the residue sits in the hydrophobic scale.33Isoleucine bein ...
... the 20 amino acids could be represented by a 5-bit binary number ranging from 00001 to 11111. To reflect a physically relevant property, such as hydrophobicity for example, the amino acids would be assigned a 5-bit number depending on where the residue sits in the hydrophobic scale.33Isoleucine bein ...
SED4 Encodes a Yeast Endoplasmic Reticulum
... from the E R (Novick et al., 1980; Nakano and Muramatsu, 1989; Kaiser and Schekman, 1990; Hicke et al., 1992; Salama et al., 1993). Five of these proteins (Secl3p, Sec31p, Sec23p, Sec24p, and Sarlp) when added in soluble form to ER membranes will drive vesicle budding (Salama et al., 1993; Barlowe e ...
... from the E R (Novick et al., 1980; Nakano and Muramatsu, 1989; Kaiser and Schekman, 1990; Hicke et al., 1992; Salama et al., 1993). Five of these proteins (Secl3p, Sec31p, Sec23p, Sec24p, and Sarlp) when added in soluble form to ER membranes will drive vesicle budding (Salama et al., 1993; Barlowe e ...
Protein domain
A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and often can be independently stable and folded. Many proteins consist of several structural domains. One domain may appear in a variety of different proteins. Molecular evolution uses domains as building blocks and these may be recombined in different arrangements to create proteins with different functions. Domains vary in length from between about 25 amino acids up to 500 amino acids in length. The shortest domains such as zinc fingers are stabilized by metal ions or disulfide bridges. Domains often form functional units, such as the calcium-binding EF hand domain of calmodulin. Because they are independently stable, domains can be ""swapped"" by genetic engineering between one protein and another to make chimeric proteins.