ACID*BASE DISORDERS
... For example, in a 70 kg patient, if the HC03- is 6 mEq/L, and it is desired to bring the HC03- to 10mEq/L: HC03- deficit = .5 X 70 X (10 mEq/L - 6 mEq/L) = 140mEqn Give this calculated amount slowly and remeasure pH, HC03- and PCO~after the HCO3- is given to assess the effect of therapy on the acid- ...
... For example, in a 70 kg patient, if the HC03- is 6 mEq/L, and it is desired to bring the HC03- to 10mEq/L: HC03- deficit = .5 X 70 X (10 mEq/L - 6 mEq/L) = 140mEqn Give this calculated amount slowly and remeasure pH, HC03- and PCO~after the HCO3- is given to assess the effect of therapy on the acid- ...
Chapter One Hemilabile Ligands in Transition
... The first type of ligand to be termed hemilabile was o-(diphenylphosphino)anisole, which is an ether-phosphine ligand. Phosphines are one of the most versatile ligands that can bind to late transition metals, since they produce stable metal-phosphorus bonds.9 Hemilabile phosphines have potentially i ...
... The first type of ligand to be termed hemilabile was o-(diphenylphosphino)anisole, which is an ether-phosphine ligand. Phosphines are one of the most versatile ligands that can bind to late transition metals, since they produce stable metal-phosphorus bonds.9 Hemilabile phosphines have potentially i ...
Renal Function Testing - American Journal of Kidney Diseases
... laboratory evaluation may be only way of detecting disease ● Tests should detect abnormalities early enough to allow corrective therapy ● Important for measuring renal disease progression and efficacy of therapies ● Help predict when renal replacement therapy may be necessary ● Aid in appropriate do ...
... laboratory evaluation may be only way of detecting disease ● Tests should detect abnormalities early enough to allow corrective therapy ● Important for measuring renal disease progression and efficacy of therapies ● Help predict when renal replacement therapy may be necessary ● Aid in appropriate do ...
Poster
... (7) Biological Significance The basic research question centered on pyruvate carboxylase is concerned with how multifunctional enzymes coordinate catalysis. Multifunctional enzymes combine two or more spatially distinct active sites to accomplish an overall reaction. Pyruvate carboxylase, a multifun ...
... (7) Biological Significance The basic research question centered on pyruvate carboxylase is concerned with how multifunctional enzymes coordinate catalysis. Multifunctional enzymes combine two or more spatially distinct active sites to accomplish an overall reaction. Pyruvate carboxylase, a multifun ...
Pathways of Glucose Assimilation in Puccinia graminis
... evaporated to dryness in a scintillation vial, 10 ml scintillant solution was added and the radioactivity was estimated. The aqueous fraction was separated into neutral, anionic and cationic fractions by ion exchange chromatography as described by Neal & Beevers (1961). The fractions were evaporated ...
... evaporated to dryness in a scintillation vial, 10 ml scintillant solution was added and the radioactivity was estimated. The aqueous fraction was separated into neutral, anionic and cationic fractions by ion exchange chromatography as described by Neal & Beevers (1961). The fractions were evaporated ...
Communication
... damage in vivo, we developed a quantitative assay for measuring levels of 3-nitrotyrosine. The method combines gas chromatography with stable isotope dilution mass spectrometry (GC-MS). Using this assay, we first investigated the relative yields of protein-bound oxidation products in bovine serum al ...
... damage in vivo, we developed a quantitative assay for measuring levels of 3-nitrotyrosine. The method combines gas chromatography with stable isotope dilution mass spectrometry (GC-MS). Using this assay, we first investigated the relative yields of protein-bound oxidation products in bovine serum al ...
Manipulating redox and ATP balancing for improved production of
... was PCR amplified and cloned under the control of its natural promoter into a low copy vector pSmart-LC-Kan from Lucigen Corp. The ASKA clone plasmid carrying the E.coli PPC under the control of inducible lac promoter (JW3928) was also utilized for the overexpression studies (Kitagawa et al., 2005). ...
... was PCR amplified and cloned under the control of its natural promoter into a low copy vector pSmart-LC-Kan from Lucigen Corp. The ASKA clone plasmid carrying the E.coli PPC under the control of inducible lac promoter (JW3928) was also utilized for the overexpression studies (Kitagawa et al., 2005). ...
Urinary Organic Acids - Peirson Center for Children
... An Organic Acid profile can be utilised as an effective screening tool for areas of abnormality that may not be able to be determined from patient history alone. By using multiple metabolic markers in conjunction with one another, a sophisticated, targeted and individualised treatment plan can be mo ...
... An Organic Acid profile can be utilised as an effective screening tool for areas of abnormality that may not be able to be determined from patient history alone. By using multiple metabolic markers in conjunction with one another, a sophisticated, targeted and individualised treatment plan can be mo ...
The interplay between metabolic homeostasis and
... studies now indicate that ALS patients have impairments in whole body physiology and energy homeostasis, with data suggesting that an imbalance in energy metabolism appears to negatively influence the rate of progression of disease [14, 36–38, 40, 41, 45–62]. Should this be the case, attempts to off ...
... studies now indicate that ALS patients have impairments in whole body physiology and energy homeostasis, with data suggesting that an imbalance in energy metabolism appears to negatively influence the rate of progression of disease [14, 36–38, 40, 41, 45–62]. Should this be the case, attempts to off ...
Investigation of asparagine deamidation in a SOD1
... acidic environment (pH ~ 4). The digestions with endoproteinase Glu-C (pH 4 and 8) and endoproteinase AspN (pH 8) were performed in an analogous way for the purified precursor. MS analysis followed after 4 h and 22 h of digestion. Mass spectrometry. MALDI-TOF MS experiments were performed in a refle ...
... acidic environment (pH ~ 4). The digestions with endoproteinase Glu-C (pH 4 and 8) and endoproteinase AspN (pH 8) were performed in an analogous way for the purified precursor. MS analysis followed after 4 h and 22 h of digestion. Mass spectrometry. MALDI-TOF MS experiments were performed in a refle ...
BILE ACIDS - Liquid Chromatography
... gas chromatography as the butyl ester-acetate derivatives. Journal of Chromatography 415: 13}26. Grundy SM, Ahrens Jr EH and Mietinnen TA (1965) Quantitative isolation and gas-liquid chromatographic analysis of total faecal bile acids. Journal of Lipid Research 6: 397}410. Kuksis A (1976) Gas chroma ...
... gas chromatography as the butyl ester-acetate derivatives. Journal of Chromatography 415: 13}26. Grundy SM, Ahrens Jr EH and Mietinnen TA (1965) Quantitative isolation and gas-liquid chromatographic analysis of total faecal bile acids. Journal of Lipid Research 6: 397}410. Kuksis A (1976) Gas chroma ...
Tricarboxylic Acid Cycle-Dependent Regulation of Staphylococcus
... icaR (34, 35, 52). In addition, the luxS quorum-sensing system has a minimal effect on PIA synthesis and biofilm formation; however, the mechanism remains to be determined (82). In Staphylococcus aureus, B is activated during stress conditions and growth phase transitions (3, 81). Because S. epider ...
... icaR (34, 35, 52). In addition, the luxS quorum-sensing system has a minimal effect on PIA synthesis and biofilm formation; however, the mechanism remains to be determined (82). In Staphylococcus aureus, B is activated during stress conditions and growth phase transitions (3, 81). Because S. epider ...
Electrolytes and metabolic disorder.
... Lactate may exceed glucose as oxidative substrate A number of studies support the role of lactate as a preferred oxidative substrate in stressed myocardium [13-16]. Half-molar lactate well-tolerated, & increases CO post-bypass surgery Lactate not harmful – but potentially helpful ...
... Lactate may exceed glucose as oxidative substrate A number of studies support the role of lactate as a preferred oxidative substrate in stressed myocardium [13-16]. Half-molar lactate well-tolerated, & increases CO post-bypass surgery Lactate not harmful – but potentially helpful ...
Title Metabolism of fluoroorganic compounds in microorganisms
... stereospecifically degrade the amino acid L-4-fluoroglutamic acid (Donnelly and Murphy 2007). In cell free extract ammonia and fluoride are produced from 4-fluoroglutamic acid, and although the enzyme(s) involved have not been identified, this reaction appears to be gratuitous, since it occurs in ot ...
... stereospecifically degrade the amino acid L-4-fluoroglutamic acid (Donnelly and Murphy 2007). In cell free extract ammonia and fluoride are produced from 4-fluoroglutamic acid, and although the enzyme(s) involved have not been identified, this reaction appears to be gratuitous, since it occurs in ot ...
XPS depth Profiling with the new MAGCIS cluster ion source, from
... Alternating Phe/Tyr layers, with layer of Phe on top surface and 3 Tyr layers Top Phe layer not observed Damaged BEFORE analysis Quantification change between Phe and Tyr as expected Slight increase in carbon signal over 300nm depth (1.2 At%) Chemical resolution of Phe and Tyr oxygen th ...
... Alternating Phe/Tyr layers, with layer of Phe on top surface and 3 Tyr layers Top Phe layer not observed Damaged BEFORE analysis Quantification change between Phe and Tyr as expected Slight increase in carbon signal over 300nm depth (1.2 At%) Chemical resolution of Phe and Tyr oxygen th ...
THE EFFECT OF HYBRIDIZATION ON METABOLISM AND HYPOXIA TOLERANCE IN SUNFISH
... Production of cellular energy in the form of ATP is critical for virtually all processes in an organism. The balance between ATP synthesis and ATP utilization is an important factor that determines a cell’s ability to survive and function. ATP turnover can vary dramatically between tissues, and thus ...
... Production of cellular energy in the form of ATP is critical for virtually all processes in an organism. The balance between ATP synthesis and ATP utilization is an important factor that determines a cell’s ability to survive and function. ATP turnover can vary dramatically between tissues, and thus ...
The use of deuteration for the structural study of larger proteins
... • typically up to 5Å but further possible (slower spin diffusion and longer selective T1 (diagonal signal) • very long mixing times ( < 1.2 s) → up to 8Å inter-proton distances. (Mal et al., J. Biomol. NMR 1998, 12, 259-276) • Not enough restraints to calculate accurate global folds (RMSD > 8Å). Par ...
... • typically up to 5Å but further possible (slower spin diffusion and longer selective T1 (diagonal signal) • very long mixing times ( < 1.2 s) → up to 8Å inter-proton distances. (Mal et al., J. Biomol. NMR 1998, 12, 259-276) • Not enough restraints to calculate accurate global folds (RMSD > 8Å). Par ...
Document
... Add substrate to a prep and look for intermediates and end products If substrate is radiolabeled (3H, 14C) it’s easier, but even nonradioactive isotopes can be used for mass spectrometry and NMR NMR on protons, 13C, 15N, 31P Reproduce reactions using isolated substrates and enzymes ...
... Add substrate to a prep and look for intermediates and end products If substrate is radiolabeled (3H, 14C) it’s easier, but even nonradioactive isotopes can be used for mass spectrometry and NMR NMR on protons, 13C, 15N, 31P Reproduce reactions using isolated substrates and enzymes ...
Enzyme Mechanisms - Illinois Institute of Technology
... Add substrate to a prep and look for intermediates and end products If substrate is radiolabeled (3H, 14C) it’s easier, but even nonradioactive isotopes can be used for mass spectrometry and NMR NMR on protons, 13C, 15N, 31P Reproduce reactions using isolated substrates and enzymes ...
... Add substrate to a prep and look for intermediates and end products If substrate is radiolabeled (3H, 14C) it’s easier, but even nonradioactive isotopes can be used for mass spectrometry and NMR NMR on protons, 13C, 15N, 31P Reproduce reactions using isolated substrates and enzymes ...
PURINE Lacture
... 1. Negative regulation of PRPP Synthatase & PRPP Amidotransferase is lost 2. PRPP levels are increased because of defects in salvage pathways Therefore, there is net increase in biosynthetic/degradation pathways!! ...
... 1. Negative regulation of PRPP Synthatase & PRPP Amidotransferase is lost 2. PRPP levels are increased because of defects in salvage pathways Therefore, there is net increase in biosynthetic/degradation pathways!! ...
accelerate adc dar characterization
... implementation so that you can take advantage of the power of automation in days rather than months. You can also use the AssayMAP Bravo platform to purify ADCs from complex matrices such as serum. The first variation of this workflow is ADC deglycosylation. This analytical approach provides the int ...
... implementation so that you can take advantage of the power of automation in days rather than months. You can also use the AssayMAP Bravo platform to purify ADCs from complex matrices such as serum. The first variation of this workflow is ADC deglycosylation. This analytical approach provides the int ...
Lipotoxicity in steatohepatitis occurs despite an increase in
... size) with the mobile phases as follows: 1) 60:40 acetonitrile-water (mobile phase A) and 2) 90:10 isopropanol-acetonitrile (mobile phase B), with 10 mM ammonium formate and 0.1% formic acid in both phases A and B. The gradient started at 32% phase B and rose to 100% phase B over 16 min, remaining i ...
... size) with the mobile phases as follows: 1) 60:40 acetonitrile-water (mobile phase A) and 2) 90:10 isopropanol-acetonitrile (mobile phase B), with 10 mM ammonium formate and 0.1% formic acid in both phases A and B. The gradient started at 32% phase B and rose to 100% phase B over 16 min, remaining i ...
Autocatalytic Sets in E. coli Metabolism
... which case they are treated as synthesized organic cofactors); iii) Reactions catalyzed by a protein that has no known or annotated cofactor are defined as being catalyzed by a general catalyst called “Protein”, which is included in the food set. In case of RNA-dependent reactions, a generic “RNA” c ...
... which case they are treated as synthesized organic cofactors); iii) Reactions catalyzed by a protein that has no known or annotated cofactor are defined as being catalyzed by a general catalyst called “Protein”, which is included in the food set. In case of RNA-dependent reactions, a generic “RNA” c ...
Human Angiotensin Converting Enzyme 2 (ACE 2) ELISA
... determination of human ACE2 in biological fluids. A polyclonal antibody specific for ACE2 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ACE2 is recognize ...
... determination of human ACE2 in biological fluids. A polyclonal antibody specific for ACE2 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ACE2 is recognize ...