Inhibition of RNA Synthesis by Anthracycline Analogs
... of anthracyclines in the treatment of leukemias and other forms of cancer. The biological and pharmacological activities of these drugs appear to be, at least in part, due to their ability to interact with DNA. ...
... of anthracyclines in the treatment of leukemias and other forms of cancer. The biological and pharmacological activities of these drugs appear to be, at least in part, due to their ability to interact with DNA. ...
P-Element Transformation with period Locus DNA Restores
... The transformed strains are named (leftmost column) with respect to the lengths In krlobases of per-region subsegments (see Frgure IA) used to produce me vectors that allowed generation of the transformants, Each strain also is named by the addition of an arbitrary number to desrgnate it as independ ...
... The transformed strains are named (leftmost column) with respect to the lengths In krlobases of per-region subsegments (see Frgure IA) used to produce me vectors that allowed generation of the transformants, Each strain also is named by the addition of an arbitrary number to desrgnate it as independ ...
A mixed group ll/group III twintron in the Euglena
... (16). The sizes at the right are based on the characterized PCR products. (Middle left panel) DNA sequence analysis of the unspliced rps3 cDNA clone, pEZC 1022. A portion of the cDNA primer retained during cloning is indicated. The arrow refers to the position of the internal group II intron-extemal ...
... (16). The sizes at the right are based on the characterized PCR products. (Middle left panel) DNA sequence analysis of the unspliced rps3 cDNA clone, pEZC 1022. A portion of the cDNA primer retained during cloning is indicated. The arrow refers to the position of the internal group II intron-extemal ...
How were introns inserted into nuclear genes?
... proteases and calcium-binding proteins have apparentinserted by a mechanism that no longer exists in the ly been inserted into highly conserved coding regions, limited range of phyla 'that have been studied within which certain nucleotides must always have although more extensive study of Protista, ...
... proteases and calcium-binding proteins have apparentinserted by a mechanism that no longer exists in the ly been inserted into highly conserved coding regions, limited range of phyla 'that have been studied within which certain nucleotides must always have although more extensive study of Protista, ...
Small Nucleolar RNA
... rRNAs contain 110 2'-Omethyl groups and almost 100 pseudouridines • snoRNAs direct methyltransferase and pseudouridine synthase enzymes to the appropriate rRNA nucleotide ...
... rRNAs contain 110 2'-Omethyl groups and almost 100 pseudouridines • snoRNAs direct methyltransferase and pseudouridine synthase enzymes to the appropriate rRNA nucleotide ...
MicroRNAs: key participants in gene regulatory networks
... the biosynthesis and functions of miRNAs, was a predicted target of miR168, suggesting a negative feedback mechanism to control expression of Argonaute and activities of miRNAs [33]. The high percentage of predicted miRNA targets acting as developmental regulators and the conservation of target si ...
... the biosynthesis and functions of miRNAs, was a predicted target of miR168, suggesting a negative feedback mechanism to control expression of Argonaute and activities of miRNAs [33]. The high percentage of predicted miRNA targets acting as developmental regulators and the conservation of target si ...
Combination of Reverse Transcription and Multienzyme Restriction
... DNA from viable cells and that from dead cells. In addition to the ethidium monoazide bromide PCR method, reverse-transcription PCR (RT-PCR) can differentiate between viable and dead bacteria because most mRNAs have short half-lives in dead bacteria. Although RT-PCR is time consuming and requires hi ...
... DNA from viable cells and that from dead cells. In addition to the ethidium monoazide bromide PCR method, reverse-transcription PCR (RT-PCR) can differentiate between viable and dead bacteria because most mRNAs have short half-lives in dead bacteria. Although RT-PCR is time consuming and requires hi ...
Finding Regulatory Motifs
... stretching 100-1000 bp upstream of the transcriptional start site. • Located within the RR are the Transcription Factor Binding Sites (TFBS), also known as motifs, which are specific for a given transcription factor. • TFs influence gene expression by binding to a specific TFBS. • A TFBS can be loca ...
... stretching 100-1000 bp upstream of the transcriptional start site. • Located within the RR are the Transcription Factor Binding Sites (TFBS), also known as motifs, which are specific for a given transcription factor. • TFs influence gene expression by binding to a specific TFBS. • A TFBS can be loca ...
Thesis-1965R-K29r
... composition of the nucleolus is chiefly RNA and proteins. It may also contain certain enzymes and I ipids. The synthesis of RNA in the nucleolus is generally accepted. The types are not agreed on. The transfer of RNA to the cytoplasm seems probable but the method is not apparent. Protein synthesis w ...
... composition of the nucleolus is chiefly RNA and proteins. It may also contain certain enzymes and I ipids. The synthesis of RNA in the nucleolus is generally accepted. The types are not agreed on. The transfer of RNA to the cytoplasm seems probable but the method is not apparent. Protein synthesis w ...
Force spectroscopy of single DNA and RNA molecules Mark C
... formed during stretching. Transitions to this intermediate state are indicated by green arrows in (a) and (b). (Reproduced with permission from [17••].) ...
... formed during stretching. Transitions to this intermediate state are indicated by green arrows in (a) and (b). (Reproduced with permission from [17••].) ...
pyrimidine
... Laser fluorescence detection allows for primer identification in real time An automated sequencing machine can handle 4500 bases/hour That’s one of the technologies that has made large-scale sequencing projects like the human genome project possible ...
... Laser fluorescence detection allows for primer identification in real time An automated sequencing machine can handle 4500 bases/hour That’s one of the technologies that has made large-scale sequencing projects like the human genome project possible ...
lac
... mutated, will grow on lactose. • However they make β-galactosidase all of the time. These mutants that have lost the ability to control gene expression are called constitutive mutants. They are also recessive. ...
... mutated, will grow on lactose. • However they make β-galactosidase all of the time. These mutants that have lost the ability to control gene expression are called constitutive mutants. They are also recessive. ...
The E-Class PPR Protein MEF3 of Arabidopsis
... Fig. 3 Identification of the Arabidopsis thaliana MEF3 gene. (A) Introduction of the A. thaliana Col versions of the two candidate genes for PPR proteins in the mapped genomic window into Ler protoplasts shows differential effects. Sequence analysis of the atp4 cDNA shows that gene At1g06140 strongl ...
... Fig. 3 Identification of the Arabidopsis thaliana MEF3 gene. (A) Introduction of the A. thaliana Col versions of the two candidate genes for PPR proteins in the mapped genomic window into Ler protoplasts shows differential effects. Sequence analysis of the atp4 cDNA shows that gene At1g06140 strongl ...
Transcription regulation of the Escherichia coli pcnB gene coding for
... to be more abundant and of higher physiological importance than pppGpp, it is often mentioned as the main stringent response factor. In fact, ppGpp can directly and indirectly regulate expression of variety of genes, important for survival of bacterial cells. It interacts with RNA polymerase (Artsim ...
... to be more abundant and of higher physiological importance than pppGpp, it is often mentioned as the main stringent response factor. In fact, ppGpp can directly and indirectly regulate expression of variety of genes, important for survival of bacterial cells. It interacts with RNA polymerase (Artsim ...
in plant physiology
... subcellular localization was determined (RpoTp: chloroplasts, RpoTm: mitochondria, RpoTmp: chloroplast and mitochondria) (Hedtke et al., 1997; Weihe and Borner, 1999; Hedtke et al., 2000) (Fig. 1A). RpoTmp and RpoTp likely represent nuclear encoded RNA polymerase enzyme in chloroplasts (NEP) (review ...
... subcellular localization was determined (RpoTp: chloroplasts, RpoTm: mitochondria, RpoTmp: chloroplast and mitochondria) (Hedtke et al., 1997; Weihe and Borner, 1999; Hedtke et al., 2000) (Fig. 1A). RpoTmp and RpoTp likely represent nuclear encoded RNA polymerase enzyme in chloroplasts (NEP) (review ...
Chapter 12 Molecular Genetics
... The enzyme DNA polymerase catalyzes the addition of appropriate nucleotides to the new DNA strand. The nucleotides are added to the 3' end of the new strand, as illustrated in Figure 12.11. DNA polymerase continues adding new DNA nucleotides to the chain by adding to the 3' end of the new DNA strand ...
... The enzyme DNA polymerase catalyzes the addition of appropriate nucleotides to the new DNA strand. The nucleotides are added to the 3' end of the new strand, as illustrated in Figure 12.11. DNA polymerase continues adding new DNA nucleotides to the chain by adding to the 3' end of the new DNA strand ...
When epigenetics meets alternative splicing: the roles of DNA
... exon 5 of the CD45 gene is methylation sensitive; exon 5 is included in the mRNA only when it is not methylated [16]. Since CTCF binds to a specific sequence in a small subset of exons, there must be other splicing regulatory proteins that are recruited by methyl binding proteins and deposited on th ...
... exon 5 of the CD45 gene is methylation sensitive; exon 5 is included in the mRNA only when it is not methylated [16]. Since CTCF binds to a specific sequence in a small subset of exons, there must be other splicing regulatory proteins that are recruited by methyl binding proteins and deposited on th ...
here - Genomes Unzipped
... correct genomic location of a sequencing read. We have not attempted to distinguish between these two types of artifact in this analysis, as both are non-biological. For the test of position bias, it is known that the error rate of Illumina sequencing depends on the position in the read [6]. Additi ...
... correct genomic location of a sequencing read. We have not attempted to distinguish between these two types of artifact in this analysis, as both are non-biological. For the test of position bias, it is known that the error rate of Illumina sequencing depends on the position in the read [6]. Additi ...
RNA Interference and Small Interfering RNAs
... analysis. The breakthrough in the identification of the sequencespecific mediator came when an unexpectedly short (approximately 25 nucleotides) abundant RNA species was identified in cosuppressing or virus-infected plants. This RNA corresponded to both the sense and antisense sequences of the cosup ...
... analysis. The breakthrough in the identification of the sequencespecific mediator came when an unexpectedly short (approximately 25 nucleotides) abundant RNA species was identified in cosuppressing or virus-infected plants. This RNA corresponded to both the sense and antisense sequences of the cosup ...
Virginia State Science Olympiad Regional Tournament
... Two (2) non-programmable non-graphing calculators, one (1) standard-size (8.5”x11”) double-sided sheet (not two one-side sheets) of paper containing any information, and writing implements may be used. Any other electronic devices are not allowed for this event; please consult the proctor about a sa ...
... Two (2) non-programmable non-graphing calculators, one (1) standard-size (8.5”x11”) double-sided sheet (not two one-side sheets) of paper containing any information, and writing implements may be used. Any other electronic devices are not allowed for this event; please consult the proctor about a sa ...
Mitochondrial genes in the colourless alga Prototheca wickerhamii
... intron number is considerable. The gene for subunit I of the cytochrome oxidase (coxl), for example, contains 16 introns in the filamentous fungus Podospora anserina and up to seven introns in various strains of the yeast Saccharomyces cerevisiae, but none in the common laboratory strain of Neurospo ...
... intron number is considerable. The gene for subunit I of the cytochrome oxidase (coxl), for example, contains 16 introns in the filamentous fungus Podospora anserina and up to seven introns in various strains of the yeast Saccharomyces cerevisiae, but none in the common laboratory strain of Neurospo ...
Modeling Spatial Correlation of DNA Deformation
... As suggested by the experimental studies of ref 11, here we assume that one base pair with index n = 0 is pulled apart along its long side. This deformation causes an interhelical distance change that involves backbone chemical bonds, stacking interactions, and hydrogen bonds. Because the stiffness o ...
... As suggested by the experimental studies of ref 11, here we assume that one base pair with index n = 0 is pulled apart along its long side. This deformation causes an interhelical distance change that involves backbone chemical bonds, stacking interactions, and hydrogen bonds. Because the stiffness o ...
Rolling circle transcription on smallest size double stranded DNA
... structures as opposed the information carrying role the molecule fills in nature. DNA has diverse potential applications such as molecular computing, as a scaffold serving as structural guides for other molecules or as programmable assembly chains. I will here describe the principles of using DNA as ...
... structures as opposed the information carrying role the molecule fills in nature. DNA has diverse potential applications such as molecular computing, as a scaffold serving as structural guides for other molecules or as programmable assembly chains. I will here describe the principles of using DNA as ...
This article appeared in a journal published by Elsevier. The
... NLS(s) but still within the arginine-rich region (ARR) located in the N-terminal half of the 2b protein [9]. Based on RNA sequence data, CMV strains can be classified into three subgroups, IA, IB and II [20]. It has been found that subgroup I strains are, in general, more virulent than subgroup II st ...
... NLS(s) but still within the arginine-rich region (ARR) located in the N-terminal half of the 2b protein [9]. Based on RNA sequence data, CMV strains can be classified into three subgroups, IA, IB and II [20]. It has been found that subgroup I strains are, in general, more virulent than subgroup II st ...
FISH or CISH methods for In situ hybridization
... Simultaneous detection of expression of three genes in a whole mount Drosophila melanogaster embryo by fluorescence in situ hybridization (FISH) using the FISH Tag™ RNA Multicolor Kit. Green: sog (short gastrulation) labeled with Alexa Fluor® 488 dye; red: ftz (fushi tarazu) labeled with Alexa Fluor ...
... Simultaneous detection of expression of three genes in a whole mount Drosophila melanogaster embryo by fluorescence in situ hybridization (FISH) using the FISH Tag™ RNA Multicolor Kit. Green: sog (short gastrulation) labeled with Alexa Fluor® 488 dye; red: ftz (fushi tarazu) labeled with Alexa Fluor ...
Nucleic acid tertiary structure
The tertiary structure of a nucleic acid is its precise three-dimensional structure, as defined by the atomic coordinates. RNA and DNA molecules are capable of diverse functions ranging from molecular recognition to catalysis. Such functions require a precise three-dimensional tertiary structure. While such structures are diverse and seemingly complex, they are composed of recurring, easily recognizable tertiary structure motifs that serve as molecular building blocks. Some of the most common motifs for RNA and DNA tertiary structure are described below, but this information is based on a limited number of solved structures. Many more tertiary structural motifs will be revealed as new RNA and DNA molecules are structurally characterized.