Structural organization of the transfer RNA gene clusters of cholera
... environment of the host bacteria. The major metabolic shift is directed towards the rapid macromolecular synthesis necessary for phage development. Translation of the phage messenger RNA takes place on the preexisting host ribosomes, using in most cases, host aminoacyl-tRNA synthetases and tRNAs. Ho ...
... environment of the host bacteria. The major metabolic shift is directed towards the rapid macromolecular synthesis necessary for phage development. Translation of the phage messenger RNA takes place on the preexisting host ribosomes, using in most cases, host aminoacyl-tRNA synthetases and tRNAs. Ho ...
mRNA Export - e
... •CRM1 itself does not bind to RNA, instead recruiting NEScontaining adaptor proteins that bind directly to RNA or to other RBPs. For example, AU-rich elements are recognized by RBP Huantigen R (HuR; also known as ELAVL1) and its protein ligands, which interact with CRM1 ...
... •CRM1 itself does not bind to RNA, instead recruiting NEScontaining adaptor proteins that bind directly to RNA or to other RBPs. For example, AU-rich elements are recognized by RBP Huantigen R (HuR; also known as ELAVL1) and its protein ligands, which interact with CRM1 ...
An accessible database for mouse and human whole transcriptome
... Quantitative polymerase chain reaction (qPCR) is an efficient tool to quantify changes in abundance of DNA/RNA molecules. Its main advantage is that it has a large dynamic range and requires only basic sample preparation steps (reverse transcription of RNA). Double-stranded DNA dye (such as SYBR) ba ...
... Quantitative polymerase chain reaction (qPCR) is an efficient tool to quantify changes in abundance of DNA/RNA molecules. Its main advantage is that it has a large dynamic range and requires only basic sample preparation steps (reverse transcription of RNA). Double-stranded DNA dye (such as SYBR) ba ...
5 CHAPTER 2: LITERATURE REVIEW 2.1 Types of Ribonucleic
... Like cell transcriptional process, the DNA containing miRNAs sequences can reside within intergenic or intronic regions of coding sequence, untranslated region or exonic regions of non-coding sequence. These are transcribed into long miRNA primary transcripts (primiRNAs) by RNA polymerase II (Ambros ...
... Like cell transcriptional process, the DNA containing miRNAs sequences can reside within intergenic or intronic regions of coding sequence, untranslated region or exonic regions of non-coding sequence. These are transcribed into long miRNA primary transcripts (primiRNAs) by RNA polymerase II (Ambros ...
Downloaded - Laboratoire de Chimie Théorique
... found in the Mg2+ structure. Crystal structures for Poll with Na+ in the active site have been previously reported.29 These structures, along with kinetic results,8 suggest that Na+ inhibits catalysis by forming a catalytically inactive structure.29 In the experimental structure, the reported nucleo ...
... found in the Mg2+ structure. Crystal structures for Poll with Na+ in the active site have been previously reported.29 These structures, along with kinetic results,8 suggest that Na+ inhibits catalysis by forming a catalytically inactive structure.29 In the experimental structure, the reported nucleo ...
evolution and mechanism of translation in chloroplasts
... ranging from 5 kb to 76 kb in length (84). This arrangement results in duplication of the rRNA genes and other genes included within the IRs. Most of the size variation among ctDNAs in land plants can be accounted for by changes in the length of the IR. For example, geranium ctDNA is unusually large ...
... ranging from 5 kb to 76 kb in length (84). This arrangement results in duplication of the rRNA genes and other genes included within the IRs. Most of the size variation among ctDNAs in land plants can be accounted for by changes in the length of the IR. For example, geranium ctDNA is unusually large ...
Choosing the Right Centrifuge for Your - Laboratory
... g-forces of up to 802,000 × g and volumes up to 250 mL; and microultracentrifuges, which support g-forces of above 1,000,000 × g and microvolume samples up to 13.5 mL. Common ultracentrifuge applications include the separation of virus particles; DNA, protein, or RNA fractionation; as well as lipopr ...
... g-forces of up to 802,000 × g and volumes up to 250 mL; and microultracentrifuges, which support g-forces of above 1,000,000 × g and microvolume samples up to 13.5 mL. Common ultracentrifuge applications include the separation of virus particles; DNA, protein, or RNA fractionation; as well as lipopr ...
Jigsaw handout - the Biology Scholars Program Wiki
... binds to lac repressor and causes a conformation change that alters the structure of lac repressor so that it cannot bind to DNA. Therefore, in the presence of lactose, lac repressor does not bind to the operator and RNA polymerase can transcribe the genes in the lac operon. Case study (homework or ...
... binds to lac repressor and causes a conformation change that alters the structure of lac repressor so that it cannot bind to DNA. Therefore, in the presence of lactose, lac repressor does not bind to the operator and RNA polymerase can transcribe the genes in the lac operon. Case study (homework or ...
Epigenetic inheritance of acquired traits through sperm RNAs and
... Acquisitive sperm — information flow The production of functional sperm begins with spermatogenesis in the testis, which is followed by matur ation in the epididymis; each stage involves complex Box 2 | Transvection and paramutation in mice Transvection occurs during chromosome pairing such as dur ...
... Acquisitive sperm — information flow The production of functional sperm begins with spermatogenesis in the testis, which is followed by matur ation in the epididymis; each stage involves complex Box 2 | Transvection and paramutation in mice Transvection occurs during chromosome pairing such as dur ...
Molecular events during translocation and proofreading extracted
... ble for power management of both translocation and processive active site switching. Once this oscillation is established, each leaving pyrophosphate (PPi) bound to the fingers in the closed conformation replenishes energy to maintain the oscillation. With each push from a newly cleaved PPi resultin ...
... ble for power management of both translocation and processive active site switching. Once this oscillation is established, each leaving pyrophosphate (PPi) bound to the fingers in the closed conformation replenishes energy to maintain the oscillation. With each push from a newly cleaved PPi resultin ...
D. melanogaster - GEP Community Server
... Transcript Details tab of the Gene Record Finder Use placement of the flanking exons to reduce the size of the search region if possible Increase sensitivity of nucleotide searches Change Program Selection to blastn Change Word size to 7 Change Match/Mismatch Scores to +1, -1 Change Gap Costs to Exi ...
... Transcript Details tab of the Gene Record Finder Use placement of the flanking exons to reduce the size of the search region if possible Increase sensitivity of nucleotide searches Change Program Selection to blastn Change Word size to 7 Change Match/Mismatch Scores to +1, -1 Change Gap Costs to Exi ...
Abundant RNA editing sites of chloroplast protein
... The evolutionary pattern of RNA editing is another interesting topic. Some scholars believe that the RNA editing phenomenon is a relic of ancient RNA world and is involved in primordial error correction, such as repairing UV damage at the transcript level. Others argue that the editing system produc ...
... The evolutionary pattern of RNA editing is another interesting topic. Some scholars believe that the RNA editing phenomenon is a relic of ancient RNA world and is involved in primordial error correction, such as repairing UV damage at the transcript level. Others argue that the editing system produc ...
Full-Text PDF
... acid sequence level with Trm11, respectively, and has the same modular architecture as yeast Trm11. Two studies have linked defaults in the human TRMT11 gene or transcript with advanced prostate cancer [35,36], and this protein was shown to interact with at least three proteins from the p53-family i ...
... acid sequence level with Trm11, respectively, and has the same modular architecture as yeast Trm11. Two studies have linked defaults in the human TRMT11 gene or transcript with advanced prostate cancer [35,36], and this protein was shown to interact with at least three proteins from the p53-family i ...
Control of Growth and of the Nuclear Division Cycle in Neurospora
... zoopfii (126), and in the slime mold Physarium polycephalum (124). Many data are also available on the yeast S. cerevisiae. Two groups of workers (19, 185) have shown that RNA per cell (or the RNA/DNA ratio) varies with the growth rate in batch cultures, although in a nonlinear fashion, and less sha ...
... zoopfii (126), and in the slime mold Physarium polycephalum (124). Many data are also available on the yeast S. cerevisiae. Two groups of workers (19, 185) have shown that RNA per cell (or the RNA/DNA ratio) varies with the growth rate in batch cultures, although in a nonlinear fashion, and less sha ...
Antisense Transcript and RNA Processing
... polyadenylate tract, resulting in this strain being unable to conduct photosynthesis. A collection of photosynthetic revertants was obtained from D26pAtE, and gel blot hybridizations revealed RNA processing alterations in the majority of these suppressor of polyadenylation (spa) strains, resulting i ...
... polyadenylate tract, resulting in this strain being unable to conduct photosynthesis. A collection of photosynthetic revertants was obtained from D26pAtE, and gel blot hybridizations revealed RNA processing alterations in the majority of these suppressor of polyadenylation (spa) strains, resulting i ...
Nucleotide sequence and genome organization of foot-and
... These difficulties seem to be related to the internal poly(C) tract, which, although readily copied into cDNA (7), is probably highly unstable in E. coli, as shown for other (GtC)-homopolymers longer than 30 basepairs (19). In accordance with this notion we have only been able to clone 11 C residues ...
... These difficulties seem to be related to the internal poly(C) tract, which, although readily copied into cDNA (7), is probably highly unstable in E. coli, as shown for other (GtC)-homopolymers longer than 30 basepairs (19). In accordance with this notion we have only been able to clone 11 C residues ...
Document
... one in every 25 bases is edited on average, which leads to about 1 in every 8 codons being edited on average. In plant mitochondrial mRNAs, about 2% of the nucleotides are edited on average. In the remainder of this article, we briefly describe the editing events in these organisms. Then we focus fi ...
... one in every 25 bases is edited on average, which leads to about 1 in every 8 codons being edited on average. In plant mitochondrial mRNAs, about 2% of the nucleotides are edited on average. In the remainder of this article, we briefly describe the editing events in these organisms. Then we focus fi ...
Review over DNA, RNA, proteins, viruses, bacteria, DNA technology
... Essential knowledge 3.A.1: DNA, and in some cases RNA, is the primary source of heritable information. b. DNA and RNA molecules have structural similarities and differences that define function. [See also 4.A.1] Evidence of student learning is a demonstrated understanding of each of the following: 1 ...
... Essential knowledge 3.A.1: DNA, and in some cases RNA, is the primary source of heritable information. b. DNA and RNA molecules have structural similarities and differences that define function. [See also 4.A.1] Evidence of student learning is a demonstrated understanding of each of the following: 1 ...
Chapter 14
... 14.9 The DNA-Binding Form of the Lambda Repressor Is a Dimer • Binding to the operator requires the dimeric form so that two DNA-binding domains can contact the operator simultaneously. • Cleavage of the repressor between the two ...
... 14.9 The DNA-Binding Form of the Lambda Repressor Is a Dimer • Binding to the operator requires the dimeric form so that two DNA-binding domains can contact the operator simultaneously. • Cleavage of the repressor between the two ...
Applications of Bioinformatics and Genomics/Proteomics
... uploaded on the Internet the same day; Location: HEB Rm 127; every Wednesday and Friday; Time 10am -12pm. Office hours: Every Wednesday and Friday from 9 AM to 10 AM in the office of Dr. Fedorov (Room 308 at Health Science building, HSC; tel: 419-383-5270). Also, students may contact remotely via Sk ...
... uploaded on the Internet the same day; Location: HEB Rm 127; every Wednesday and Friday; Time 10am -12pm. Office hours: Every Wednesday and Friday from 9 AM to 10 AM in the office of Dr. Fedorov (Room 308 at Health Science building, HSC; tel: 419-383-5270). Also, students may contact remotely via Sk ...
CoevolPaper2 - University of Illinois Archives
... 2.2 Evolution of Signatures in the 23S rRNA While phylogenetic analyses of 16S rRNA sequences have been widely used as a molecular taxonomic measure, studies of the 23S rRNA have been evolutionarily confined to a small number of taxa. Yet the 23S rRNA should prove at least as informative of the evol ...
... 2.2 Evolution of Signatures in the 23S rRNA While phylogenetic analyses of 16S rRNA sequences have been widely used as a molecular taxonomic measure, studies of the 23S rRNA have been evolutionarily confined to a small number of taxa. Yet the 23S rRNA should prove at least as informative of the evol ...
X chromosome inactivation- Review
... Three steps1. choice- occurs in embryonic cells Xist is expressed from both X chromosomes in female Xist encodes 15 kb polyadenylated untranslated RNA that is unstable Xist is gene located within Xic ...
... Three steps1. choice- occurs in embryonic cells Xist is expressed from both X chromosomes in female Xist encodes 15 kb polyadenylated untranslated RNA that is unstable Xist is gene located within Xic ...
Messenger RNA reprogramming by spliceosome-mediated
... The third component in the spliceosome-mediated trans-splicing reactions is the PTM. PTMs can be designed to carry out one of three forms of trans-splicing, depending on the type of trans-splicing domain in the PTM (Figure 5) (see discussions in refs. 4, 9). These domains provide PTMs with the cis e ...
... The third component in the spliceosome-mediated trans-splicing reactions is the PTM. PTMs can be designed to carry out one of three forms of trans-splicing, depending on the type of trans-splicing domain in the PTM (Figure 5) (see discussions in refs. 4, 9). These domains provide PTMs with the cis e ...
A DEAD Box RNA Helicase Is Essential for mRNA Export and
... These results suggest that the cryophyte mutation may have impaired the production of a germination inhibitor under high temperatures. In addition, the mutation appears to have less impact on the development of younger seedlings compared with older ones under high temperatures. We also tested the ge ...
... These results suggest that the cryophyte mutation may have impaired the production of a germination inhibitor under high temperatures. In addition, the mutation appears to have less impact on the development of younger seedlings compared with older ones under high temperatures. We also tested the ge ...
Ribosome Profiling
... expression by analyzing entire transcripts can provide important insights into the spatial and temporal gene regulation inside a cell. Microarray and RNA sequencing (RNA-seq) are two extensively used techniques to analyze genome wide abundances of transcripts. It is pertinent to note that regulation ...
... expression by analyzing entire transcripts can provide important insights into the spatial and temporal gene regulation inside a cell. Microarray and RNA sequencing (RNA-seq) are two extensively used techniques to analyze genome wide abundances of transcripts. It is pertinent to note that regulation ...
Nucleic acid tertiary structure
The tertiary structure of a nucleic acid is its precise three-dimensional structure, as defined by the atomic coordinates. RNA and DNA molecules are capable of diverse functions ranging from molecular recognition to catalysis. Such functions require a precise three-dimensional tertiary structure. While such structures are diverse and seemingly complex, they are composed of recurring, easily recognizable tertiary structure motifs that serve as molecular building blocks. Some of the most common motifs for RNA and DNA tertiary structure are described below, but this information is based on a limited number of solved structures. Many more tertiary structural motifs will be revealed as new RNA and DNA molecules are structurally characterized.