pdf file - John Innes Centre
... proteins do indeed form a complex. To be of physiological significance the model required that GlnK binding to AmtB should be sensitive, rapid, and reversible. We now report experiments that confirm all three of these properties. We also show that AmtB plays an active part in this process such that ...
... proteins do indeed form a complex. To be of physiological significance the model required that GlnK binding to AmtB should be sensitive, rapid, and reversible. We now report experiments that confirm all three of these properties. We also show that AmtB plays an active part in this process such that ...
Interrelations between translation and general mRNA degradation in
... However, recent studies suggest that the inverse relationship between translation and mRNA degradation may be more nuanced. One example is that in response to translational inhibition from stresses, yeast and other eukaryotes inhibit the mRNA decay process, which could allow cells to assess new envi ...
... However, recent studies suggest that the inverse relationship between translation and mRNA degradation may be more nuanced. One example is that in response to translational inhibition from stresses, yeast and other eukaryotes inhibit the mRNA decay process, which could allow cells to assess new envi ...
Protein Interaction Technical Handbook
... These processes include cell-cycle control, differentiation, protein folding, signaling, transcription, translation, post-translational modification and transport. Implications about function can be made via protein:protein interaction studies. These implications are based on the premise that the fu ...
... These processes include cell-cycle control, differentiation, protein folding, signaling, transcription, translation, post-translational modification and transport. Implications about function can be made via protein:protein interaction studies. These implications are based on the premise that the fu ...
Distinct C-terminal Amino Acid Sequence Motifs Serve
... Localization, in BY2 cells, of TraB with mutations to the amino acid residues peripheral to the dibasic targeting signal motif............................................................ Flow chart depicting the various steps involved in the identification of putative A. thaliana TA OEPs............ ...
... Localization, in BY2 cells, of TraB with mutations to the amino acid residues peripheral to the dibasic targeting signal motif............................................................ Flow chart depicting the various steps involved in the identification of putative A. thaliana TA OEPs............ ...
ppt file
... Key points about the character of amino acid side chains • amino acids don’t fall neatly into classes--they are different combinations of small/large, charged/uncharged, polar/nonpolar properties • how we casually speak of them can affect the way we ...
... Key points about the character of amino acid side chains • amino acids don’t fall neatly into classes--they are different combinations of small/large, charged/uncharged, polar/nonpolar properties • how we casually speak of them can affect the way we ...
REVIEWS - Unisciel
... Kettins (f) are 0.5–0.7 MDa85,86 and ~180-nm long93. They are composed mainly of immunoglobulin (Ig) domains joined by relatively long linker sequences of 35 residues. Towards their carboxyl terminus they also have a unique sequence insert. Some, if not all, kettin isoforms are likely to form elasti ...
... Kettins (f) are 0.5–0.7 MDa85,86 and ~180-nm long93. They are composed mainly of immunoglobulin (Ig) domains joined by relatively long linker sequences of 35 residues. Towards their carboxyl terminus they also have a unique sequence insert. Some, if not all, kettin isoforms are likely to form elasti ...
5 The structure and function of membrane proteins
... length in this text. The area seemed complete. How wrong I was and how wrong an undergraduate’s perception can be! The last 30 years have seen an explosion in the area of protein biochemistry so that my 1975 edition of Biochemistry by Albert Lehninger remains, perhaps, of historical interest only. T ...
... length in this text. The area seemed complete. How wrong I was and how wrong an undergraduate’s perception can be! The last 30 years have seen an explosion in the area of protein biochemistry so that my 1975 edition of Biochemistry by Albert Lehninger remains, perhaps, of historical interest only. T ...
post- translational modifications
... for the synthesis of PTM peptides and proteins. Post-translational modifications (PTMs) enable the cell to change the properties and activities of proteins after expression. Most of the PTMs are catalyzed by specialized enzymes. Practically all proteinogenic amino acids can be modified, thus PTMs co ...
... for the synthesis of PTM peptides and proteins. Post-translational modifications (PTMs) enable the cell to change the properties and activities of proteins after expression. Most of the PTMs are catalyzed by specialized enzymes. Practically all proteinogenic amino acids can be modified, thus PTMs co ...
Full Article - PDF - Journal of Biotech Research
... Folic acids are essential cofactors for synthesis of DNA, RNA, membrane lipids, methionine metabolism and neurotransmitter synthesis. P. falciparum is capable of synthesizing folate de novo or obtaining it through salvage, whereas humans can only obtain folate from their diet, making the folate path ...
... Folic acids are essential cofactors for synthesis of DNA, RNA, membrane lipids, methionine metabolism and neurotransmitter synthesis. P. falciparum is capable of synthesizing folate de novo or obtaining it through salvage, whereas humans can only obtain folate from their diet, making the folate path ...
Motor proteins of the kinesin superfamily
... common core nucleotide-binding region with kinesin, but with less structural overlap (six -strands and four -helices). The most striking similarities are seen in the regions directly adjacent to the ADP-binding site, including the Ploop and two other highly conserved motifs, called switch I and sw ...
... common core nucleotide-binding region with kinesin, but with less structural overlap (six -strands and four -helices). The most striking similarities are seen in the regions directly adjacent to the ADP-binding site, including the Ploop and two other highly conserved motifs, called switch I and sw ...
Pericentriolar material structure and dynamics
... complexes with SAS-4, Cnn and g-tubulin, all proteins discovered to occupy concentric toroidal domains within the interphase PCM [31]. These data suggest that the organization and size of the interphase PCM is established through the deposition of pre-assembled complexes of defined stoichiometry and ...
... complexes with SAS-4, Cnn and g-tubulin, all proteins discovered to occupy concentric toroidal domains within the interphase PCM [31]. These data suggest that the organization and size of the interphase PCM is established through the deposition of pre-assembled complexes of defined stoichiometry and ...
Structure and Function Relationships between ATPase Family, AAA
... Figure 11. Hits from crystallization trials for complex of PCNA and a synthetic peptide derived from ATAD5 ........................................................................................................ 45 Figure 12. Optimization of the crystallization condition for the crystallization of P ...
... Figure 11. Hits from crystallization trials for complex of PCNA and a synthetic peptide derived from ATAD5 ........................................................................................................ 45 Figure 12. Optimization of the crystallization condition for the crystallization of P ...
Article (Published version)
... animal cap region to favor targeting to the neural plate and ectoderm, bypassing effects on the role of Gli2 and Gli3 in mesoderm (Brewster et al., 2000). Diffusion of the injected MOs (25 bp) was different than that of the injected lacZ RNA (~3000 bp; not shown), making the precise localization of ...
... animal cap region to favor targeting to the neural plate and ectoderm, bypassing effects on the role of Gli2 and Gli3 in mesoderm (Brewster et al., 2000). Diffusion of the injected MOs (25 bp) was different than that of the injected lacZ RNA (~3000 bp; not shown), making the precise localization of ...
Queuosine formation in eukaryotic tRNA occurs via a mitochondria
... stop codon was converted to an Ala, underlined) according to the Superscript III protocol (Invitrogen) for gene specific primers. First-strand products were separated from reaction components using a nucleotide removal kit (Qiagen). TGT and Qv1 were amplified by PCR using the aforementioned reverse ...
... stop codon was converted to an Ala, underlined) according to the Superscript III protocol (Invitrogen) for gene specific primers. First-strand products were separated from reaction components using a nucleotide removal kit (Qiagen). TGT and Qv1 were amplified by PCR using the aforementioned reverse ...
Insights into interactions between poly(ethylene glycol) and proteins
... inertness with protein surfaces8-9. Dextran is a mostly linear polymer composed of glucose monomers linked via α-(1,6)-D-glycosidic bonds (approximately 95%) with an occasional α-(1,3)-D-glycosidic linkage10-12. ...
... inertness with protein surfaces8-9. Dextran is a mostly linear polymer composed of glucose monomers linked via α-(1,6)-D-glycosidic bonds (approximately 95%) with an occasional α-(1,3)-D-glycosidic linkage10-12. ...
Biomarker discovery for psychiatric disorders
... Although over the last century tremendous progress has been made in the therapy and mortality decrease of devastating conditions such as cancer and cardiopathies, no decrease has been observed in mortality rates or overall prevalence of psychiatric disorders (Kessler et al., 2005). It is therefore n ...
... Although over the last century tremendous progress has been made in the therapy and mortality decrease of devastating conditions such as cancer and cardiopathies, no decrease has been observed in mortality rates or overall prevalence of psychiatric disorders (Kessler et al., 2005). It is therefore n ...
Divergent Functional Properties of the Ribosome
... al., 1996; Wang et al., 1998; Pellecchia et al., 2000). This domain consists of eight antiparallel -strands, which form a hydrophobic pocket in which peptide substrate can bind. This domain has been shown to be sufficient to bind substrate in vitro (Wang et al., 1993; Pellecchia et al., 2000). The ...
... al., 1996; Wang et al., 1998; Pellecchia et al., 2000). This domain consists of eight antiparallel -strands, which form a hydrophobic pocket in which peptide substrate can bind. This domain has been shown to be sufficient to bind substrate in vitro (Wang et al., 1993; Pellecchia et al., 2000). The ...
Interactions of Elongation Factor 1 Cell Protrusions with F-Actin and
... actin to maintain actin filament polymerization over a period of time longer than the duration of the initial protrusion. As a result, the cells would have a slower rate of migration. Alternatively, the site of actin synthesis (and of other functionally related proteins) may affect the location of n ...
... actin to maintain actin filament polymerization over a period of time longer than the duration of the initial protrusion. As a result, the cells would have a slower rate of migration. Alternatively, the site of actin synthesis (and of other functionally related proteins) may affect the location of n ...
Identification and analysis of new phloem proteins from
... transport mainly mineral-containing water from the roots to the shoots, while the phloem is responsible for the translocation of organic compounds from the sites of synthesis (source organs) to the developing and non-photosynthetic tissues (sink organs). These two transport systems differ not only i ...
... transport mainly mineral-containing water from the roots to the shoots, while the phloem is responsible for the translocation of organic compounds from the sites of synthesis (source organs) to the developing and non-photosynthetic tissues (sink organs). These two transport systems differ not only i ...
Complement Levels and Activity in the Normal and LPS - AJP-Lung
... proteins enhance bacterial clearance by opsonizing bacteria and enhancing ingestion by phagocytes. Bacteria may also be directly lysed by complement proteins. In addition complement cleavage products act as chemoattractants to recruit immune cells to the site of infection where they can further cont ...
... proteins enhance bacterial clearance by opsonizing bacteria and enhancing ingestion by phagocytes. Bacteria may also be directly lysed by complement proteins. In addition complement cleavage products act as chemoattractants to recruit immune cells to the site of infection where they can further cont ...
- Free Documents
... both cases it is the gain or loss oI phosphate groups that determines whether the protein is active or inactive. The largest class consists oI proteins that are activated or inactivated by . For these proteins, the switch is thrown in one Chapter phosphorylation discussed in direction by a protein k ...
... both cases it is the gain or loss oI phosphate groups that determines whether the protein is active or inactive. The largest class consists oI proteins that are activated or inactivated by . For these proteins, the switch is thrown in one Chapter phosphorylation discussed in direction by a protein k ...
as a PDF - CiteSeerX
... enable receptors such as integrins to directly or indirectly interact with tyrosine or serine/threonine kinases to transmit signals. First identified by ten Hoeve et al,13 CrkL is a 39-kd adapter protein that consists of 1 SH2 and 2 SH3 domains and is 60% homologous to CrkII. CrkL is most abundantly ...
... enable receptors such as integrins to directly or indirectly interact with tyrosine or serine/threonine kinases to transmit signals. First identified by ten Hoeve et al,13 CrkL is a 39-kd adapter protein that consists of 1 SH2 and 2 SH3 domains and is 60% homologous to CrkII. CrkL is most abundantly ...
Modulation of functional properties of bifunctional S- Adenosylmethionine decarboxylase/Ornithine decarboxylase of
... Tumor necrosis factor alpha ...
... Tumor necrosis factor alpha ...
assembly of integral membrane proteins from the periplasm into the
... unfolded OMPs on affinity columns (Chen and Henning, 1996). E. coli cells lacking the skp gene display reduced levels of OmpA, OmpC, OmpF, and LamB in the OM (Chen and Henning, 1996; Missiakas et al., 1996), a phenotype which resembles that of surA mutants (Missiakas et al., 1996; Rouvière and Gross ...
... unfolded OMPs on affinity columns (Chen and Henning, 1996). E. coli cells lacking the skp gene display reduced levels of OmpA, OmpC, OmpF, and LamB in the OM (Chen and Henning, 1996; Missiakas et al., 1996), a phenotype which resembles that of surA mutants (Missiakas et al., 1996; Rouvière and Gross ...
Lecture 1 - "Hudel" Luecke
... http://bass.bio.uci.edu/~hudel/bs99a/lecture20/lecture1_1.html (1 of 4)5/24/2007 12:49:06 PM ...
... http://bass.bio.uci.edu/~hudel/bs99a/lecture20/lecture1_1.html (1 of 4)5/24/2007 12:49:06 PM ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.