Molecular Characterization of Visual Pigments in
... Total RNAs of T. granarius were extracted from an anteriorpart ofthecarapace forRT-PCRandfrom thecompound eyes and ocelli for RACE. RNA of T. longicaudatus was extracted from the compound eyes and ocelli and for B. kugenumaensis, from the whole head including the compound eyes and ocelli. Clone deve ...
... Total RNAs of T. granarius were extracted from an anteriorpart ofthecarapace forRT-PCRandfrom thecompound eyes and ocelli for RACE. RNA of T. longicaudatus was extracted from the compound eyes and ocelli and for B. kugenumaensis, from the whole head including the compound eyes and ocelli. Clone deve ...
Chance and Necessity in Arthur Peacocke`s Scientific Work
... branched or forked or just a single helix, whether once disassociated (denatured) DNA strands would re-associate (renature) and other similar questions. Peacocke’s studies with intercalating dyes set the stage for his own discoveries in this area. Furthermore, Peacocke investigated the denaturation ...
... branched or forked or just a single helix, whether once disassociated (denatured) DNA strands would re-associate (renature) and other similar questions. Peacocke’s studies with intercalating dyes set the stage for his own discoveries in this area. Furthermore, Peacocke investigated the denaturation ...
Optimal estimation of diffusion coefficients from single
... paramount for the precise understanding of these processes. Recent developments in fluorescent labels have made it possible to track diffusion of single molecules, e.g., proteins on biopolymers such as DNA [1–3] or microtubules [4–6], on surfaces [7], in lipid membranes [8–10], and inside cells [11– ...
... paramount for the precise understanding of these processes. Recent developments in fluorescent labels have made it possible to track diffusion of single molecules, e.g., proteins on biopolymers such as DNA [1–3] or microtubules [4–6], on surfaces [7], in lipid membranes [8–10], and inside cells [11– ...
Characterization of the cDNA and Gene Coding for the Biotin
... Ward (personal communication) and to the partia1 sequence reported in GenBank accession number L34413. The major differences between these three sequences are at the 5' and 3' noncoding ends. At the 5' end, the pBS-1 sequence is 122 nucleotides longer than the sequence shown in GenBank accession num ...
... Ward (personal communication) and to the partia1 sequence reported in GenBank accession number L34413. The major differences between these three sequences are at the 5' and 3' noncoding ends. At the 5' end, the pBS-1 sequence is 122 nucleotides longer than the sequence shown in GenBank accession num ...
Journal of Applied Microbiology
... of the 16S and the 5¢ end of the 23S rRNA genes was obtained by the band stab amplification technique, in which resolved PCR products, based on the 1491f (5¢-GAA GTC GTA ACA AGG TA-3¢) and L1r [5¢-CA(A/G) GGC ATC CAC CGT-3¢] primers, were retrieved directly from an agarose gel with a pipet tip aft ...
... of the 16S and the 5¢ end of the 23S rRNA genes was obtained by the band stab amplification technique, in which resolved PCR products, based on the 1491f (5¢-GAA GTC GTA ACA AGG TA-3¢) and L1r [5¢-CA(A/G) GGC ATC CAC CGT-3¢] primers, were retrieved directly from an agarose gel with a pipet tip aft ...
SALSA MLPA probemix P222-A2 LCA mix-2 - MRC
... chromosome 12q21.33, ~88 Mb from the p-telomere and spans ~93 kb of genomic DNA. Its mutation c.2991+1655AG seem to be an important cause of LCA and may explain up to 21% of LCA cases. (den Hollander et al., Am J Hum Genet, 2006). The P222 probemix contains 2 probes for the CEP290 gene. One probe i ...
... chromosome 12q21.33, ~88 Mb from the p-telomere and spans ~93 kb of genomic DNA. Its mutation c.2991+1655AG seem to be an important cause of LCA and may explain up to 21% of LCA cases. (den Hollander et al., Am J Hum Genet, 2006). The P222 probemix contains 2 probes for the CEP290 gene. One probe i ...
ABO genotyping in leukemia patients reveals new ABO variant alleles
... (DiaMed Latino America), polyclonal anti-A, anti-B, anti-AB (Fresenius Hemocare, SP, Brazil), and lectin anti-A1 and monoclonal anti-H (Gamma Biologicals, Houston, TX, USA). All reagents were used as recommended by the manufacturer’s instructions. ABO reverse typing was conducted by agglutination te ...
... (DiaMed Latino America), polyclonal anti-A, anti-B, anti-AB (Fresenius Hemocare, SP, Brazil), and lectin anti-A1 and monoclonal anti-H (Gamma Biologicals, Houston, TX, USA). All reagents were used as recommended by the manufacturer’s instructions. ABO reverse typing was conducted by agglutination te ...
History of Discoveries in Molecular Biology
... invented a technique for multiplying DNA sequences in vitro by, the polymerase chain reaction - PCR. PCR has been called the most revolutionary new technique in molecular biology in the 1980s. Cetus patented the process, and in the summer of 1991 sold the patent to Hoffman-La Roche, Inc. for $300 mi ...
... invented a technique for multiplying DNA sequences in vitro by, the polymerase chain reaction - PCR. PCR has been called the most revolutionary new technique in molecular biology in the 1980s. Cetus patented the process, and in the summer of 1991 sold the patent to Hoffman-La Roche, Inc. for $300 mi ...
Product description P003-D1 MLH1-MSH2-v01 - MRC
... Confirmation of results: Copy number changes detected by only a single probe always require confirmation by another method. An apparent deletion detected by a single probe can be due to e.g. a mutation/polymorphism that prevents ligation or destabilises the binding of probe oligonucleotides to the D ...
... Confirmation of results: Copy number changes detected by only a single probe always require confirmation by another method. An apparent deletion detected by a single probe can be due to e.g. a mutation/polymorphism that prevents ligation or destabilises the binding of probe oligonucleotides to the D ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... average DNA concentration (0.161±0.002µg/µl) was determined from absorbance at 260 nm (Jenway, Genova Model, UK). All samples had a 260/280 nm absorbance ratio between 1.4 and 1.63. The integrity of the DNA was checked by electrophoresis on 2 % agarose gel stained with ethidium bromide. Genotyping o ...
... average DNA concentration (0.161±0.002µg/µl) was determined from absorbance at 260 nm (Jenway, Genova Model, UK). All samples had a 260/280 nm absorbance ratio between 1.4 and 1.63. The integrity of the DNA was checked by electrophoresis on 2 % agarose gel stained with ethidium bromide. Genotyping o ...
Sequence variation characteristics of D
... growing cells, the proportion of mtDNA can be as high as 18% of total cellular DNA. Plants show an extremely wide range of variation in mtDNA size, with a minimum size of 100 kb. The function of the additional DNA, in comparison with mammalian mtDNA, has not been defined decisively, but it seems lik ...
... growing cells, the proportion of mtDNA can be as high as 18% of total cellular DNA. Plants show an extremely wide range of variation in mtDNA size, with a minimum size of 100 kb. The function of the additional DNA, in comparison with mammalian mtDNA, has not been defined decisively, but it seems lik ...
Analysis of the genetic potential of the spongederived fungus
... Ircinia fasciculata in the course of a research program focused on the discovery and characterization of novel natural products. This led to the isolation and characterisation of two novel and structurally highly similar polyketides: sorbicillactone A and sorbicillactone B. Consistent with their str ...
... Ircinia fasciculata in the course of a research program focused on the discovery and characterization of novel natural products. This led to the isolation and characterisation of two novel and structurally highly similar polyketides: sorbicillactone A and sorbicillactone B. Consistent with their str ...
Distribution and Concordance of N-Acetyltransferase Genotype and
... 37°C for 3 h and then incubated at 65°C for 1 h. Fifteen ml of digested product were electrophoresed at 100 V for 30 min and then at 75 V for 30 min on a 3% agarose gel stained with ethidium bromide. Although the triple-digest method was an efficient means of analysis, an unanticipated band of ;400 ...
... 37°C for 3 h and then incubated at 65°C for 1 h. Fifteen ml of digested product were electrophoresed at 100 V for 30 min and then at 75 V for 30 min on a 3% agarose gel stained with ethidium bromide. Although the triple-digest method was an efficient means of analysis, an unanticipated band of ;400 ...
A Physical Gene Map of the Bacteriophage P22 Late
... Therefore SmaI site 2 is located at the other end of the 2.0-kb SmaI fragment at P22 map coordinate 0.958. A packaging fragment extending from pat to SmaI site 1 should be present. However, due to the small size (less than 200 base pairs) and low yield of this fragment, it has not been detected. Xho ...
... Therefore SmaI site 2 is located at the other end of the 2.0-kb SmaI fragment at P22 map coordinate 0.958. A packaging fragment extending from pat to SmaI site 1 should be present. However, due to the small size (less than 200 base pairs) and low yield of this fragment, it has not been detected. Xho ...
WOTD - Brookwood High School
... V: incomplete dominance neither allele is dominant to the other, both are expressed equally, often a blending of the two traits occurs ...
... V: incomplete dominance neither allele is dominant to the other, both are expressed equally, often a blending of the two traits occurs ...
Consulta: subjectFacets:"16S rRNA" Registros recuperados: 43
... A reverse transcriptase - polymerase chain reaction based assay for Borrelia species detection in ticks was developed. The method was based on amplification of 552 nucleotide bases long sequence of 16S rRNA, targeted by Borrelia specific primers. In the present study, total RNA extracted from Ixodes ...
... A reverse transcriptase - polymerase chain reaction based assay for Borrelia species detection in ticks was developed. The method was based on amplification of 552 nucleotide bases long sequence of 16S rRNA, targeted by Borrelia specific primers. In the present study, total RNA extracted from Ixodes ...
- UEA Digital Repository
... Non-methylotrophic, methylated amine-utilising bacteria ...................... 47 ...
... Non-methylotrophic, methylated amine-utilising bacteria ...................... 47 ...
Cloning, Sequencing, and Characterization of Luciola italica
... The characteristic yellow-green light of a firefly is the result of a multi-step reaction catalyzed by the luciferase enzyme. This enzyme has many applications in the biomedical field and ongoing work is being done to alter its properties to better fit these applications. The purpose of this project ...
... The characteristic yellow-green light of a firefly is the result of a multi-step reaction catalyzed by the luciferase enzyme. This enzyme has many applications in the biomedical field and ongoing work is being done to alter its properties to better fit these applications. The purpose of this project ...
DNA
... • Experiments performed by Fredrick Griffith in 1928 and experiments with bacteriophages in 1952 led to this discovery. (BILD 1 Lecture, UCSD,Fall 2003) • DNA provides a code, consisting of 4 letters, for all cellular function. ...
... • Experiments performed by Fredrick Griffith in 1928 and experiments with bacteriophages in 1952 led to this discovery. (BILD 1 Lecture, UCSD,Fall 2003) • DNA provides a code, consisting of 4 letters, for all cellular function. ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).