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Chapter 13 Reading Guide Section 13.1: page 337 Name: ______________________ Period: ______ Date: ______ 1. Give one examples of selective breeding from the book: __________________________________. 2. Give one advantage of inbreeding: ___________________________________________________. Give one disadvantage of inbreeding: _________________________________________________. 3. True or false. The homozygous genotypes are considered purebred. 4. Which of the following genotypes is considered hybrid? a. GG b. gg c. Gg d. homozygous 5. Describe two advantages of hybrid plants given in the textbook: _______________________________ _____________________________________________________________________________________ 6. Which of the following is an example of a test cross? Circle one. a. BB x Bb b. BB x bb c. Bb x Bb d. Bb x ?? 7. In a test cross, the parent with the known genotype is always ________________. 8. Understanding a main idea question: Hybrid corn is produced that is both resistant to bacterial infection and produces a high number of ears of corn. What is the most likely phenotype of each parent plant for this hybrid plant? (Think each parent contributed one of the genes) a. both were resistant to bacterial infection b. one was resistant to bacterial infection and one produced many ears of corn c. both had produces high number of ears of corn d. they did not have any special phenotype – just chosen at random 9. Understanding the main idea question. A test cross is made between a cat with a dominant phenotype but an unknown genotype (could be BB or Bb) and a cat with a recessive genotype (bb). The mating produces 10 kittens of which 50% show the recessive genotype and 50% show the dominant phenotype. What is the most genotype of the parent cat? a. BB b. Bb c. bb d. brown Section 13.2: page 341 10. What is an advantage genetic engineering has over selective breeding? ________________________ 11. What example of a transgenic organism does your book give? ______________________________ 12. What are the three steps in the process of creating a transgenic organism? (first paragraph page 342) a. ________________________________________________________________________ b. ________________________________________________________________________ c. ________________________________________________________________________ 13. Describe a restriction enzyme: ________________________________________________________ 14. Which of the following sequences is a palindrome? (read the same forward and backward like MOM) a. ACCATGGT b. TTAAAATT c. GATAGATA d. TTTTAAAA 15. Some restriction enzymes produce fragments in which the DNA is cut ___________________________ both strands. These are called _______________________________. 16. Other enzymes, such as the enzyme EcoRI, cut _____________________ sequences of DNA. When this DNA is cut, double-stranded fragments with _______________________ ends are formed. 17. The restriction enzyme EcoRI cuts sticky end at the DNA sequence AATT. Mark where it would cut the following sequences: a. CGAGCTTAAGCGAG b. TCCAATATAGGAATTCC GCTCGAATTCGC TC AGGTTATATCCTTAAGG 18. Describe a vector: __________________________________________________________________ What was the vector for the transgenic tobacco plant? _____________________ 19. Give two examples of each type of vector: a. biological: _____________________________ and _______________________________ b. mechanical: ____________________________ and _______________________________ 20. What is a plasmid? ___________________________________________________________________ 21. Clones are __________________________________________________________________________. 22. What cells can plasmids deliver recombinant DNA into? _____________________________________. 23. What is a benefit of cloning animals? ____________________________________________________ _____________________________________________________________________________________ 24. The polymerase chain reaction (PCR) is used to make millions of copies of ______________________. 25. What are the uses of PCR given in the book? a. _________________________________________________________________________ b. _________________________________________________________________________ c. _________________________________________________________________________ 26. 27. What can scientists use DNA sequencing for? ______________________________________________ Briefly describe gel electrophoresis using Figure 13.8 on page 346. a. _____________________________________________________________________________ b. ______________________________________________________________________________ c. _____________________________________________________________________________ d. ______________________________________________________________________________ 28. What are two uses of recombinant DNA in industry: a. _____________________________________________________________________________ b. __________________________________________________________________________ What are two uses of recombinant DNA in medicine: a. ____________________________________________________________________________ b. _____________________________________________________________________________ What are two uses of transgenic organisms? a. ______________________________________________________________________________ b. ______________________________________________________________________________ What are two uses of recombinant DNA in agriculture? a. ______________________________________________________________________________ b. ______________________________________________________________________________ 29. 30. 31.