Download Absorbed Dose

Handout
Prokaryotes vs. Eukaryotes:
7.28
A view from the DNA. (or Why you aren.t a bacterium).
Prokaryote
Eukaryote
General:
Site of Transcription
Site of DNA Replication
Site of RNA Splicing
Site of Translation
Site of Recombination
Protoplasm
Protoplasm
Rare
Protoplasm
Protoplasm
Nucleus
Nucleus
Nucleus
Cytoplasm
Nucleus
DNA Replication:
Chromosomes
Telomeres
Circular, One Origin
No
Linear, Multiple Origins
Yes
Replication Machinery
DNA Polymerases
Primase
Helicase
Sliding Clamp
Clamp Loader
Single Stranded
DNA Binding Prot.
Polymerase Coupling
Origin Recognition
Homologous Recombination:
Strand exchange protein
DSB initiator
DNA Pol III (Replication)
DNA Pol δ, DNA Pol ε
dnaG
dnaB
DNA Pol α/Primase
MCM proteins
β-protein
PCNA
γ-complex
RF-C
SSB
RF-A
τ
dnaA
?
ORC
function in DSB repair
and genetic exchange
RecA
no specific protein
Function in DSB repair,
genetic exchange and
chromosome segragation in
meiosis.
RecA homologs (DMC1, Rad51)
Spo11
Transcription:
RNA Polymerases
Termination
Core Promoter Structure
Long Distance Activation
RNAP (Multiple σ-subunits)
Intrinsic Terminators
-35 and -10 elements
Rare
1
RNA Pol I (rRNA)
RNA Pol II (mRNA)
RNA Pol III (tRNA,
snRNA)
Poorly Understood
TATA and Initiator
Common
Handout
7.28
2
Handout
Translation:
Ribosomes
mRNA
Ribosome binding
Transcriptional Attenutation
Auxilliary Factors
Initiation
Elongation
Termination
RNA Splicing:
7.28
30S, 50S
Polycistronic
Shine/Delgarno+AUG
Yes
IF1, IF2, and IF3
EF-Tu, EF-G, EF-Ts
RF-1, RF-2, RF-3
Rare
self-splicing only
3
40S, 60S
Monocistronic
5’ Cap+AUG
No
eIF1, EIF2, eIF3, eIF4, eIF5
eEF1a, eEF2, eEF1b
eRF-1 (works with all terminators)
eRF-3
Common
Spliceosome-mediated
(+ some self-splicing
especially in organelles)