Download TQ Bank Lab 1

Lab 1 Test Bank
Lab safety and Microscope Use
LAB SAFETY
True/ False
F Open-toed shoes and shorts above the knee are allowed to wear in lab.
T Most fires in Micro lab are started from improper use of Bunsen burners and hot instruments
T The best way to deal with a fire in the lab is to just get away from it, cover it, and let it burn
out. Remember, a lab coat can be taken off and used to put out a fire if someone’s hair
catches fire, etc.
F There is no food, drink, or water bottles allowed in a micro lab, but chewing gum is okay.
F The biohazard bag is for any and all hazardous materials, including a toothpick you put in
your mouth, gloves, disposable Petri dishes, and paper towels used to dry your hands.
T For the glassware flasks and tubes, make sure all labels are removed, and all grease marks are
erased when you are done.
F If there is a spill, leave it for Andrea to clean up.
T First aid kit is in the cabinet near Andrea’s door.
Describe the correct way to pick up the microscope for use:
T The arm should face you as you lift it up. Hold with one hand under base, gently set on desk
near edge, and then turn the arm away from you. Fold up dust cover and put it in the drawer.
F When you are done with the microscope, leave the condenser in the lowest position; this is
called racking up the condenser.
T Focusing: Two knobs, coarse and fine adjustment. Fine adjustment is smaller and concentric
to the coarse adjustment knob. The coarse knob moves the stage a lot, and the fine knob
moves it a little.
T Changing the distance between the stage and the objectives to focus is called the “working
distance”.
T PARFOCAL: This term refers to the factory adjustment which means that once you are
focused with the scanning objective, you are focused with all of the objectives (except for
fine adjustment for minor corrections). If you lose focus, always go back to the scanning
objective.
1
The condenser should be racked down when viewing a slide under low power.
False
The scanning objective lens can safely be cleaned with Kim wipes. FALSE
The clarity of viewing E. coli is best by using a total magnification of 400X FALSE.
The microscopic field of view is largest at a total magnification of 1000X. FALSE
Closed toe shoes are required in science laboratories to protect feet against spills and broken
glass. TRUE
It is better to adjust the voltage knob the to control the volume of light on the slide microscope,
rather than the iris diaphragm. FALSE
The resolving power of a microscope is affected by the magnifying power of the objective lenses.
TRUE
You have prepared a wet mount of pure culture of an unknown organism. All of the organisms
are vibrating back and forth in in the center of the field. This is true motility. FALSE
Multiple Choice: Select the single best answer to the following.
Before leaving lab,
A. Be sure to put equipment back in tote box on the rack in the front of the class.
B. Make sure your area has been disinfected before and after lab
C. Make sure your microscope has been put away properly.
D. All of the above
E. A and C only
What is the function of the substage adjustment knob (left side, under stage)? What happens
when you turn it?
A. It raises and lowers the condenser.
B. It is the course focus adjustment
C. It opens or closes the iris of the condenser
D. It turns the light source on and off
E. None of the above
F. All of the above
2
The ocular is
A. the eyepiece.
B. It magnifies ten times and focuses the image on the retina.
C. There are two oculars.
D. Do not touch your eyelashes to them or they will get oil on them.
E. To clean, squirt alcohol onto lens paper and wipe, then wipe with dry lens paper.
F. All of the above
G. B, C only
H. None of the above
The ocular that is adjustable:
A. has a diopter adjustment ring (knurled knob with ridges), with plusses, minuses,
and a zero on it.
B. What happens when you move it? It moves the left ocular up and down.
C. The purpose of this is to allow the left eye to be focused independently of the right eye
D. The coarse adjustment knob is for the right eye focus.
E. If you still see two images, you have a convergence problem and you’ll need to keep one eye
closed.
F. Everyone has one dominant eye. You may need to close that eye when you want to use the
pointer, if it is on the wrong side for you.
G. Between the oculars is a disc with numbers on it. This determines the interpupillary distance
in mm (distance between your pupils).
H. All of the above
I. B, C, D only
J. None of the above
What is true about the four objective lenses:
A. The scanning (red ring) objective is the smallest, and magnifies 4x.
B. Since the ocular is 10x, the total magnification on the scanning objective is 400x.
C. The low power objective is 10x, total mag = 100x.
D. The high dry objective is 40x, total mag = 400x.
E. The oil immersion objective is l00x, total mag = 1000x.
F. All of the above
G. A, C, D, E only
H. None of the above
When first trying to identify an object on a slide for the first time:
A. Always start with the scanning objective
B. Make sure the condenser is racked up.
C. Rack up the coarse knob so the stage is all the way up.
D. Look at the slide, then lower the stage with the coarse knob until it comes into focus.
E. Only after that can you switch to the next power up (yellow low power).
F. To focus from now on, ONLY use the fine adjustment knob.
G. All of the above
H .A & B only
I. None of the above
3
Inside the condenser is a lever: the iris diaphragm lever. This opens and closes like the iris in
your eye (pupil) to regulate the amount of light allowed in. The condenser takes light from the
lamp and makes the rays into a point on the slide. The iris does the following four things:
A. Regulates light intensity
B. Contrast: (when iris is open, the contrast decreases)
C. Depth of field (when iris is open, only the foreground is in focus. When the iris is closed,
the depth of field increases and everything is in focus.
D. Resolution (sharpness of image). Resolution is best when iris is open all the way.
E. All of the above
F. D, E only
G. None of the above
Match the following objective lenses to their magnification
High dry _____ c
Scanning _____ a
Low power ______ b
Oil immersion ______ d
a) 4x
b) 10x
c) 40x
d) 100x
What does the iris diaphragm do?
a) Increases total magnification
b) Increases light when closed
c) Increases light when open
d) Adjusts the voltage intensity of the light
Place the below list of actions in the correct order:
1. _______ c
2. _______ b
3. _______ d
4. _______ e
5. _______ a
a)
b)
c)
d)
e)
Use the fine focus knob
Place slide on mechanical stage and center the specimen
Rack the stage all the way down and place the scanning objective front and center
Rack the stage up while viewing from the side
Look through the ocular eyepieces while slowly lowering the stage
The letter “e” looks upside down and backward under a microscope, and when you move the
stage to the right, it moves to the left when viewing through the ocular eyepieces. The term for
this phenomenon is ___________________ inversion
4
When switching from one objective to the next, you do not completely lose sight of the
specimen. The term for the phenomenon is ______________________ parfocal
The resolving power of a microscope is a function of
a) The magnifying power of the lenses
b) The numerical aperature (NA) of the lenses
c) The wavelength of light
d) Both (a) and (b)
e) Both (b) and (c)
What is used to clean slides?
a) Kim wipes
b) Lens paper
What is used to clean microscope lenses?
a) Kim wipes
b) Lens paper
The microscope field of view is largest at what total magnification?
a) 40x
b) 100x
c) 400x
d) 1000x
What happens to the working distance when magnification is increased?
a) Increases
b) Decreases
c) Stays the same
Which lens has the shortest working distance?
a) Scanning
b) Low power
c) High power
d) Oil lens
What is the brightfield compound microscope used for?
a. shows dark objects in a bright field
b. shows bright objects in a dark field
c. Used for seeing 3D images
d. Used for viewing live cells
The knob used for focusing with the scanning objective is called?
a. fine adjustment
b. field of vision
c. coarse adjustment
d. magnification
5
STORING THE MICROSCOPE:
The arm should face your ____1_____, the dust cover in place, the AC (power) cord is
wrapped loosely next to it, NOT wrapped around it. The condenser should be
_______2______. The _____3______ for power should be off. The ______4_____ should be
turned to the ______5________. The _____7______ should be racked down by using the
____________6__________ . The scanning objective should be in place. Clean off the oil and
other debris; wipe the ocular lens with lens paper only.
A
B
C
D
E
F
G
2.
4.
6.
3.
7.
5.
1.
Racked Up
Voltage Regulator
Course Adjustment Knob
Toggle Switch
Stage
Lowest Setting
Body
What is the fine adjustment knob used for? (choose all that apply)
a. focusing with oil immersion lenses
b. focusing with high-dry lenses
c. focusing with low power lenses
d. focusing with scanning lenses
What is it called when a microscope has only one ocular lens?
a. ocular
b. eyepiece lens
c. monocular
d. binocular
What is the smallest organism that was viewed in lab?
a. Algae
b. Fungi
c. Protozoa
d. Bacteria
What is another name for an eyepiece lens on a microscope?
a. ocular
b. monocular
c. binocular
d. objective
What is the agar powder made of?
a. seaweed
b. nutrient rich broth
c. bacteria
d. gelatin
6
1.
15.
2.
3.
4.
14.
5.
13.
6.
7,
8.
12.
11.
9.
10.
7
Answers to ABOVE diagram
1. Ocular Lenses
2. Revolving nose piece
3. Objective Lenses
4. Stage Clip
5. Stage
6. Iris Diaphragm
7. Condenser
8. Lamp
9. Base
10. Mechanical State Adjustment Knobs
11. Course Focus Knob
12. Fine Focus Knob
13. Light Intensity Knob
14. On/ Off Switch
15. Arm
Identify the parts of the microscope identified with an arrow below. In the space below the
figure, describe the function of each of the parts identified.
C
A
D
E
B
A)
B)
C)
D)
E)
Stage adjustment (or slide clamp); for holding slide on the stage
Iris diaphragm; regulates the amount of light that reaches the slide
Ocular; contains a lens which contributes to total magnification
Light Intensity Control (or Voltage knob); varies intensity of light
Stage Adjustment knob; for moving slide around on stage
8
Why is immersion oil important in seeing bacteria through the microscope?
Immersion oil is important because it helps magnify the bacteria, which are the
smallest organism. The oil helps bend the light differently to focus.
Why is it important to stain your specimen (such as cheek cells) before putting it under the
microscope?
A. There will not be much contrast if not stained, and it allows the iris to adjust better.
B. To enlarge the cells
C. This allows the cells to separate from each other which makes it easier to see under the
microscope.
D. You don’t need to stain
When preparing for a wet mount slide, how do you cover it to avoid bubbles?
A. Use the cover slip and drop it parallel to the slide.
B. Drop the cover slip down like a hinge (45 degree angle).
C. Drop the cover slip from a 90 degree angle.
What are the proper steps of cleaning the slides?
A. Throw the coverslip in the trash. Rinse the slide off with water and dry.
B. Throw the coverslip in the trash. Rinse the slide off with water and scrub the slide with
the cleanser. Dry.
C. Throw the coverslip into the broken glass container. Rinse the slide off in water at the
sink and scrub the slide down with the cleanser.
D. Throw the coverslip into the broken glass container. Rinse the slide off in water at
the sink and scrub the slide down with the cleanser. Rinse and dry and put the slides
in the disinfectant tub at the sink.
Epithelium is thick and they are not easy to break apart from each other. They do not grow back
quickly.
A. True
B. False
One of your oculars has a ____________. When you are using it, switch that ocular to the
___________eye.
A. light, weak
B. light, dominant
C. pointer, dominant
D. pointer, weak
Bacteria are small, so they always need _________ to be seen.
A. 10x
B. 100x
C. 1,000x
D. 10,000x
9
Fill in the blanks:
The oil immersion lens has the _____1 (B)______ depth of field. Start at ___2_ (E)____ and the
bacteria will look like little dots. As you progress to _____3_(AD)_____, the dots are bigger,
you can focus again. At 400x, you will start to see shapes. The details are not seen until you get
to the oil immersion lens.
A. biggest
B. narrowest
C. 10x
D. 20x
E. 40x
AB. 50x
AC. 75x
AD. 100x
AE. 200x
Why is the oil immersion lens named as such?
A. You must look through this lens only while the microscope is completely immersed in a
vat of baby oil.
B. The oil lobbyists in Washington want it that way.
C. A drop of synthetic immersion oil is placed on the slide prior to observation.
D. Tradition
How many basic shapes of bacteria are there?
A. 3
B. 2
C. 1
D. 64
Where do you place the coverslips when you are finished viewing a slide?
A. In your pocket
B. In your neighbors pocket
C. In the garbage disposal
D. In the broken glass container.
What virus may cause cervical cancer?
A. Clowns
B. Human Papillo Virus
C. The cold virus
D. The ebola virus
Which ocular should contain the pointer?
A. The ocular of your dominant eye.
B. The ocular of your nondominant eye.
C. There is no pointer
D. All of the above.
10
Which objective containers a sealer so that oil will not seep in?
A. The oil immersion lens
B. The 40x objective lens
C. The 100x objective lens
D. The 400x objective lens
What should be used to clean the slide?
A. Your sleeve
B. Alcohol
C. Saliva
D. Soda
How much does a damaged microscope objective lens cost to replace?
A. $20
B. $0
C. $60
D. $150
How do you clean the oil off the oil immersion lens?
A. You don’t.
B. Scrubbing vigorously.
C. With steel wool.
D. Dab at it with a clean paper towel until no more oil comes off.
The resolving power of a microscope is a function of
a) The magnifying power of the lenses
b) The numerical aperature (NA) of the lenses
c) The wavelength of light
d) Both (a) and (b)
e) Both (b) and (c)
When viewing an organism under the 10X objective, if you are having trouble focusing on the
specimen to observe a particular cell, you should:
a. carefully adjust the “course” adjustment knob.
b. adjust one of the ocular lenses to adjust the magnification.
c. go to a higher power lens.
d. go back to 4X.
e. two of these are correct.
When a culture is spilled, how long do you leave disinfectant on it?
a) 10 minutes
b) 15 minutes
c) 20 minutes
d) 30 minutes
11
What would be the total magnification of an object viewed with a 15x ocular and the low power
objective?
a) 100x
b) 150x
c) 300x
d) 1000x
e) 1500x
With your microscope, when changing from low to high power, it is necessary to:
a) lower the condenser
b) move the stage away from the objective lens
c) open the iris diaphragm
d) close the iris diaphragm
e) b and c are correct
What happens to the working distance when magnification is increased?
d) Increases
e) Decreases
f) Stays the same
What would be the total magnification of an object viewed with a 15x ocular and the oil
immersion objective?
a. 100X
b. 150X
c. 300X
d. 1000X
e. 1500X
When focusing your microscope, which statement is FALSE?
a. Clean all lenses with lens paper before beginning.
b. Make sure the condenser is in its highest position.
c. Always begin with a scanning objective.
d. Use the coarse adjustment knob and then the fine adjustment knob with each
objective lens.
e. Use the iris diaphragm to adjust the amount of light.
Which of the statements about immersion oil is FALSE?
a. It prevents the loss of light.
b. It is placed directly on the slide.
c. It has the same refractive index as glass.
d. It is used with the 40x objective.
e. It enhances the resolving power of the microscope.
12
Types of Media
True or False:
T: All purpose media is a nutrient agar that supports the growth of a wide variety of organisms,
but does
not support fastidious organisms.
T: The purpose of Enriched Media is used to grow FASTIDIOUS organisms, such as pathogens.
T: Jams, jellies and preserves do not develop bacteria growth because sugar acts as a bacterial
preservative.
What is an enriched media?
A. A type of media with added nutrients such as vitamins and amino acids.
B. It is used to grow bacteria which are hard to grow, known as FASTIDIOUS organisms.
C. Both A & B
Sabouraud’s Dextrose Agar (SDA)
A: is a media with high sugar content and an acidic pH.
B: SDA is considered an isolation media.
C: It favors the growth of molds and yeasts, but not bacteria.
D. A & B Only
E. All of the above
What is the difference between Broths and Slants?
A: Broths have no agar.
B: They do not have to be boiled, just stirred.
C: Slants have an agar and they are boiled.
D: After they are removed from the autoclave, they are placed on slant racks to cool so the agar
in the
tube stays at a slant.
E: A & B Only
F: All of the above
Why use a slant?
A: One can inoculate the top of a slant to get growth of aerobic bacteria
B: Or we can stab a needle of bacteria in to the tube to see if there are microbes that can grow
without air.
C: The tubes last for a long time in the refrigerator
D: The tubes are useful for making pure stock cultures.
E: A & B only
F: All of the above
What does Semi solid allows us to observe?
13
A: is microbe is motile ?
B: is the microbe is aerobic or anaerobic.
C: Both A & B
What is an agar?
A. Pathogen
B. Pure culture
C. Seaweed product
D. Bacterial culture
What is agar?
A: Seaweed product in which nutrients are added to, then heated and sterilized for the
purpose of being inoculated to encourage growth of the sample.
B: A part of the cellular structure of algae
C: An abbreviation for accelerated growth activated racemose
D: A & B only
E: All of the above
What is the purpose of Enriched Media?
A. It is used to support a wide variety of organisms.
B. It is used as a bacterial preservative.
C. It is used to grow fastidious organisms, such as pathogens.
D. It is used to isolate molds and yeasts.
E. Both C and D
What is an example of enriched media?
A. Nutrient Agar
B. Sabouraud’s Dextrose Agar
C. Nutrient Broths
D. Blood Agar
E. All of the above
F. Both B and D only
What is the content of Blood Agar?
A. Trypic Soy Agar and 5% sheep’s blood
B. 40 grams of sugar
C. Trypic Soy Agar and 10% Blood Agar
D. Stock culture
How is culture media classified?
A. By consistency and contents
B. By pH and glucose
C. By aerobic and aerobic bacteria
D. By incubation and inoculation
What is the purpose of a semi-solid consistency?
14
A. -See if the microbe is motile (can move).
B. -See if the microbe is aerobic or anaerobic.
C. -Isolate molds and yeasts
D. All of the above
E. (a and b)
What is All Purpose media?
A. A Nutrient Agar that supports fastidious organisms.
B. A Nutrient Agar that has high sugar content and an acidic pH.
C. A Nutrient Agar that supports a wide variety of organisms, except fastidious
organisms (difficult to grow bacteria).
D. None of the above.
How does Sabouraud’s Dextrose Agar sugar (SDA) act as bacterial preservative in a Selective
media?
A. SDA isolates molds and yeasts, which do well in high sugar content, whereas
bacteria is inhibited.
B. SDA provides low sugar content and a pH of 7.0.
C. SDA provides an anaerobic environment.
What is the difference between Nutrient Agar and Broths?
A. Broths have same nutrients as NA, but no agar.
B. No boiling needed, just stirred.
C. Broths are placed in tubes instead of flasks.
D. All of the above
Which statement is true about slants?
A. Slants are poured into petri dishes.
B. Slants are poured into tubes.
C. Slants are made exactly like Soy Agar.
D. Slants are have no agar.
After melting an agar tube pour into a Petri dish, why do we allow it to cool down before
pouring?
a) The organisms would die during a streak for isolation procedure
b) Too much condensation would form on the inside of the lid
c) It is too hot to handle and work with
d) All of the above
e) Both (b) and (c) are true
Lab Manual Chapter 1 and 3
15
1. If a microscope is parfocal and is in focus on the scanning lens it will be in focus on:
A. Low Power
B. High Power
C, Oil immersion
C. All of the above
2. What is the area seen through a microscope called:
A. Magnification
B. Field of vision
C. Numerical aperture
D. Refractive index
4. What are most chemoheterotrophic bacteria grown on:
A. Nutrient broth
B. Complex media
C. Petri dish
D. None of the above
5. What is the most common method of sterilizing culture media?
A. Using Lysol
B. Autoclaving
C. Heating it up
D. Using Clorox
6. What is responsible for controlling the amount of light in a microscope?
A. Objective lens
B. Body tube
C. Iris diaphragm
D. Stage
7. What type of bacterium is “rod shaped”?
A. Coccus
B. Spiral
C. Bacillus
D. Tetrad
8. An early microscope that consisted of biconvex lenses and were essentially magnifying
glasses are called __________.
A. Magnifiers
B. Simple Microscopes
C. Brightfield compound microscopes
D. objective lenses
16
9. The larger knob on the microscope used for focusing is known as the __________.
A. fine adjustment
B. coarse adjustment
C. monocular
D. ocular
10. Resolution or resolving power refers to which of the following situations?
A. The ability of lenses to reveal fine detail
B. The ability of lenses to reveal two points distinctly separated
C. The ability of the viewer to illuminate the slide
D. Both A & B
E. All of the above
11. What is a commonly used liquid complex medium?
A. Nutrient broth
B. Agar
C. Peptone
D. Distilled water
12. What is added to turn a liquid complex medium into a solid medium?
A. Peptone
B. Sodium chloride (NaCl)
C. Agar
D. Glucose
13. What is agar made of?
A. An extract from marine red algae
B. Peptone
C. Sodium chloride (NaCl)
D. Both A & C
E. All of the above
14. What is a population of cells called that arises from a single bacterial cell?
A. Turbid
B. Colony
C. Group
D. Unit
15. Intentionally introducing microbes onto nutrient agar and into nutrient broth is known as
A. Inoculation
B. Incubation
C. Colony forming
D. All of the above
17
16. Who was the first individual know to observe living microbes in suspension?
A. Antoni van Leewenhoek
B. Galileo
C. Louis Pasteur
D. Edward Jenner
E. Joseph Lister
17. Which of the following controls the angle and size of the cone of light on a microscope?
A. condenser
B. iris diaphragm
C. coarse adjustment
D. body tube
18. The basic frame of the microscope consists of the following:
A. base, stage, arm, and condenser
B. base, stage, body tube, and coarse adjustment
C. base, stage, arm, and body tube
D. base, stage, arm, and coarse adjustment
19. Higher magnification usually requires _____ light?
A. more
B. no
C. some
D. less
20. Colony descriptions for elevation include the following examples:
A. flat, convex
B. raised, umbonate
C. rhizoid, arborized
D. All of the above
E. both A and B
21. After suitable incubation, liquid media become
A. turbid or cloudy
B. solid
C. jello-like
D. none of the above
22. On ________ media, individual colonies will be visible to the naked eye.
A. liquid
B. solid
C. jello-like
D. all of the above
18
23. In a colony, a population of cells arises from
A. a single blood cell
B. a group of bacterial cells
C. a single microbe
D. a single bacterial cell
Identify the below organism will cilia all around it:
A. Paramecium
B. Coccus
C. Amoeba
Describe the below colonies:
A. Plateau
B. Convex
C. Umbonate
Petri dishes keep fresh for up to
A. one week
B. 1 month
C. 3 weeks
19
Broths are placed in
A. Tubes
B. Flasks
What acts as a bacterial preservative?
A. Mold
B. Sugar
C. Yeast
D. Salt
As you increase the magnification on the microscope, your working distance
a. decreases
b. increases
Bacteria need to be viewed under
a. 40x
b. 10x
c. 100x
Aseptic Technique
A “negative pressure room” is one in which:
a. When you open the door, air moves out of the room
b. When you open the door, air moves into the room
c. Air does not circulate to keep out foreign contaminants
d. Hazmat suits with respirators are required because there is no air
True or false: Bio Safety Level Two (BS2) microbes are potentially pathogenic (cause disease)
a. True
b. False
The best way to transfer a bacterial inoculate is to use a (an):
Inoculation loop
Inoculation needle
Your fingers
Pipette
Both A and B
True or false: The inoculation loops and needles cannot be sterilized after use and can thus
only be used once
True
False
20
Cultures in the lab can become contaminated from:
Skin
Hair
Surface contaminants
Airborne spores and living microorganisms
All of the above
True or false: Bacteria and other contaminants cannot fly
True
False
When a nurse is cleaning a wound, which technique is used?
Aseptic technique
Sterile technique
Culturing technique
Surgical technique
Aseptic technique, with regard to handling cultures is:
Equally as pure as sterile technique
More pure than sterile technique
Not as pure as sterile technique
Not an option when handling cultures
When undergoing surgery, the following technique is used:
Aseptic technique
Sterile technique
Culturing technique
Amicrobial technique
True or false: While cleaning a wound aseptically, you do not need to wear a gown or
facemask
True
False
A culture dish can be left open for a short time when viewing colonies of organisms.
True
False
An important part of Aseptic Technique is labeling. What is the correct information for your
tube or plate?
Name, Date, Bacteria, Teacher
Date, Time, Location, Class
Time, Date, Bacteria, Name
Date, Test Type, Lab Period, Name
Never grab the instrument by the handle
True False
21
To aid with the cooling of a loop, wave it around or blow on it until it is cool.
True False
When transferring cultures hold the tubes by the
Base in your dominant hand and the sterile loop in your non-dominant hand
Base in your non-dominant hand and the sterile loop in your dominant hand
Base in your dominant hand and never hold the sterile loop
Base and loop in your dominant hand only
When “flaming” a tube the angle should be at
A. 45°
B. 90°
C. 25°
D. 15°
Scoop of bacteria is known as the ______________ ?
A. Culture
B. Tube
C. Inoculum
D. Variable
All necessary equipment should be placed out of arms reach for safety
True False
When preparing the Bunsen burner,
Adjust the yellow flame so that it disappears and only the blue flame is visible
Decrease the blue flame until a yellow cone of flame is seen near the tip
Adjust the blue flame until a an orange cone of flame is seen near the tip
Increase the yellow flame so that the blue flame completely disappears
Which of the following “general considerations” is FALSE:
a. It is important to tie hair back since it can easily contaminate your specimen
b. A common way to start a fire is to reach over the flame
c. It is okay to dump microbial suspension down the drain
d. Place test tubes in the rack when working at your table
If your lab coat catches fire, what should you immediately do?
a. Throw water on it
b. Take it off and use the rest of the coat to smother the flames
c. Call 911
d. Have someone use a paper towel to smother the flames
How do you know that the gas is turned all the way on?
a. The level is parallel with the rubber tubing
b. The level is turned down
c. The level is turned up
d. The level is perpendicular to the rubber tubing
Where should microbial suspensions be thrown out?
a. In the normal trash can
22
b. In the sink
c. The lab technician will take care of it
d. Only in the discard area
Which of the following is TRUE in regards to the methods of applying an inoculum:
a. We will be using the same inoculating method for all our experiments
b. There are different techniques, thus is depends on what technique you will perform
c. It doesn’t matter what technique you utilize
d. Make sure you are using proper technique
e. Both b and d
f. None of the above
What is the purpose of streak plate?
a. To watch bacteria grow
b. To introduce a pure bacterial sample to a new plate
c. To grow contaminated bacteria
d. To store nutrient broth
When utilizing streak plate method, what is being done?
a. Spreading out the sample enough to facilitate the growth of the bacteria
b. Inoculating a dish with many species of bacteria
c. Putting nutrient broth inside the dish
d. Inoculating a dish with a virus
Which of the following “general considerations” is TRUE:
a. Don’t place pencils or pens in your working space
b. You don’t need to wear a lab coat
c. Don’t place a heated loop or glass rod into an alcohol dish
d. None of the above is true
What type of container is used for a streak isolation?
A. Flask
C. test tube
B. Petri dish
D. bowl
How many quadrants are marked on the bottom?
A. 5
C. 2
B. 3
D. 4
Which item is not used for a streak isolation?
A. Loop
D. Bunsen burner
B. Cotton swab
E. None of the above
C. agar
F. all these may be used for streak isolation
What temperature do you incubate the plate when checking for pathogenic bacteria?
A. 96.8° F
C. 62° F
B. 33° C
D. 37° C
23
Which of the following depicts the correct streak plate method:
A
C
How long do you incubate the plate?
A. 36 h
B. 72 h
In what motion do you streak the plate?
A. Circular
B. Back and forth
B (Correct)
D
C. 1h
D. 24 h
C. poking motion
D. zig zag
E. None of the above
How do you inoculate an agar slant with a loop?
A. Place the loop all the way to bottom of slant
B. Place loop straight up the slant
C. Zig zag the streak as you bring up the loop
D. All of the above
24
During a streak isolation, in which direction is the petri dish rotated?
A. Counter clockwise
C. clockwise
B. It is not rotated
D. upside down
How do you sterilize the loop?
A. In a microwave
B. Soak it in alcohol
C. In distilled water
D. Over a Bunsen burner
For each streak in each quadrant of the petri dish, the loop should be __________
A. Waved around
B. Sterilized
C. Cooled
D. Placed in ice
E. B & C
F. None of the above
Place loop on the ________________________ of the dish and tap it on the agar to make sure it
is _________________________.
A. Bottom, hard
B. Inside, pliable
C. Inside edge, cool
D. Top, cool
Uninoculated, pellicle, sediment, turbidity, flocculent are examples of what type of growth
pattern?
A. Grown patterns on slants
B. Growth patterns in broths
Why are incubating plates stored upside down?
A. Plates are not stored upside down.
B. To prevent condensation.
C. It allows the agar to fall to the bottom to prevent air from escaping.
D. It stops water from dripping onto the culture, which can ruin it.
E. Both B and D
When describing the morphology of a colony, the terms “convex”, “flat”, and “growth into
medium” are classifications of what?
A. Pigmentation
B. Configuration
C. Margin
D. Elevation
When describing the morphology of a colony, the terms “smooth”, “erose”, and “rhizoid” are
classifications of what?
A. Pigmentation
25
B. Configuration
C. Margin
D. Elevation
What are the purposes of using aseptic technique?
a) Prevent contamination of cultures
b) Prevent contamination of environment
c) Prevent infection of personnel
d) Maintain sterile conditions
e) A, B, and C are correct
ab) A, B, C, and D are correct
What part of the Bunsen burner flame is used to sterilize a loop?
a) Tip of yellow flame
b) Base of yellow flame
c) Tip of blue cone
d) Tip of red flame
Match the following grown pattern slants with their appropriate classification.
A
Beaded
Arborescent
Echinulate (spiny)
Filiform
Rhizoid
Effuse (spreading)
B
C
D
E
F
C
B
F
A
E
D
To retrieve a sample from a culture tube with an inoculating loop, the cap of the tube is
a) Removed and held in one’s teeth
b) Removed and held with the little fingers of the loop hand
26
c) Removed with the fingers of the loop hand and placed in the fingers of the tube hand
d) Removed with the fingers of the loop hand and placed on the lab bench
e) Any of the methods B, C, and D can be used.
When transferring an organism from a liquid media, what is used to obtain the organism?
a) Loop
b) Needle
c) Pipette
d) Syringe
e) Both (a) and (b) can be used
When transferring an organism from a solid media for the purpose of obtaining a pure colony,
what is used to obtain the organism?
a) Loop
b) Needle
c) Pipette
d) Syringe
e) Both (a) and (b) can be used
Which of the following is in the correct order?
a) Place organism on slide, heat fix, air dry, stain
b) Place organism on slide, blot dry, heat fix, stain
c) Place organism on slide, air dry, heat fix, stain
d) Place organism on slide, heat fix, stain, air dry
What is the purpose for doing a capsule stain? ________________________________
To outline a capsule for viewing
You should always shake a tube of culture broth before removing a sample. Why?
a. It stuns the bacteria so they are temporarily non-motile during the transfer.
b. It enrages the bacteria so they attack and cling to the inoculating loop.
c. It disperses bacteria that might otherwise have sunk to the bottom of the tube.
d. all of the above are good reasons to shake a tube of broth.
e. Actually, you should never shake a tube of broth before removing a sample.
27