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SUPPLEMENTARY MATERIALS
for
Spectroscopic Investigation and Molecular Modeling on
Porphyrin/PAMAM Supramolecular Adduct
Maria Angela Castriciano,*1 Andrea Romeo, 2 Nicola Angelini, 3 Norberto Micali, 4 Salvatore
Guccione, 5 Luigi Monsù Scolaro *2
1.
ISMN-CNR Salita Sperone n.31 Vill.S.Agata, 98166 Messina, Italy, 2. Dipartimento di Chimica
Inorganica, Chimica Analitica e Chimica Fisica, University of Messina, and C.I.R.C.M.S.B. Salita
Sperone n.31 Vill. S.Agata, 98166 Messina, Italy, 3. ISM-CNR , AdR Roma 1 Via Salaria, Km 29.3,
CP10 I-00016, Monterotondo Scalo, (RM), Italy, 4. IPCF-CNR Salita Sperone, Contrada Papardo, Faro
Superiore, 98158 Messina, Italy, 5.Dipartimento di Scienze Farmaceutiche, University of Catania, Viale
Andrea Doria 6, Ed. 2 I-95125, Catania, Italy.
*Corresponding author e-mail: [email protected] (Maria Angela Castriciano)
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Figure S1. UV/vis spectra of TPPC@PAMAM in aqueous solution at different concentrations.
[TPPC@PAMAM] = 3M (solid line), [TPPC@PAMAM] = 3mM (dashed line, spectrum made using
0.001 cm path length quartz cell) in phosphate buffer 1mM pH 7, T = 298 K.
Figure S2. UV/vis spectra of TPPC@PAMAM and relative deconvolution.
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Figure S3. Absorbance of 410 nm (empty circle) and 422nm (fully circle) deconvoluted species at
different [PAMAM] / [TPPC] stoichiometric ratios.
Figure S4. Fluorescence decays of TPPC@PAMAM in aqueous solution at 298 K, together with the fit
curves (the straight lines) and the weighted residuals.
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Figure S5. Anisotropy decays for TPPC sodium salt in aqueous solution, the curve fit (straight line) is
plotted.
Figure S6. Anisotropy decays for TPPC sodium salt in DMSO/water 1/1 mixture solvents and urea 6M,
the curve fit (straight line) is plotted.
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Figure S7. Anisotropy decays for TPPC@PAMAM (1/1) in aqueous solution, the curve fit (straight
line) is plotted.
Figure S8. Anisotropy decays for TPPC@PAMAM (1/50) in aqueous solution, the curve fit (straight
line) is plotted.
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Figure S9. UV/Vis spectra of DNA in presence of TPPC@PAMAM at various r = [TPPC@PAMAM]
/ [DNA] (base pairs) values. [DNA] = 50 M, r = 0 – 2.2, phosphate buffer 1 mM pH 7, [NaCl] = 10
mM , T = 298 K.
Figure S10. Anisotropy decay for TPPC@PAMAM in a 1/1 ratio in the presence of DNA in aqueous
solution; the curve fit (straight line) is plotted.
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