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Transcript
UNIT - I INTRODUCTION TO THE SCIENCE OF MICROBIOLOGY Major Division of Microbial World and Relationship among Them. UNIT - II CLASSIFICATION OF MICROBES Classification of Microbes and Study of Bacteria, Fungi, Yeasts, Actinomycetes, Virus, Rickettsia and Spirochaetes, Study of Mode of Transmission and Treatment of Microbial Diseases like, Cholera, Typhoid, Tuberculosis, Diphtheria, Tetanus, Syphilis and AIDS. UNIT - III NUTRITIONAL REQUIREMENT OF MICROORGANISMS Nutritional Requirements, Growth and Cultivation of Bacteria and Virus, Study of Different Important Media Required for the Growth of Aerobic and Anaerobic Bacteria and Fungi. Differential Media, Enriched Media and Selective Media, Maintenance of Laboratory Cultures. UNIT - IV SELECTION OF BACTERIA Different Methods used in Isolation and Identification of Bacteria with Emphasis on Different Staining Techniques and Biochemical Reactions. Counting of Bacteria. Total and Viable Count. Bacteriological Analysis of Drinking Water. UNIT - V STERILIZATION Detailed Study of Different Methods of Sterilization Including their Merits and Demerits, Sterilization of Equipments, Validation of Sterilization. Sterilization Methods for all Pharmaceutical Products. Detailed Study of Sterility Testing of Different Pharmaceutical Preparations. UNIT - VI DISINFECTANTS Study of Disinfectants, Antiseptics, Fungicidal and Virucidal Agents, Factors Affecting their Activities and Mechanism of Action, Evaluation of Bactericidal, Bacteriostatic, Virucidal Activities, Evaluation of Preservatives in Pharmaceutical Preparations. UNIT - VII GENETICS Introduction to Genetics, Phenotypic and Genotypic Changes in Bacteria, Mutations, Genetic Exchange in Bacteria-Transformation, Transduction and Conjugation. UNIT - VIII GENETIC ENGINEERING Study of Vector, Plasmids, Transposons, Cosmids, Restriction Endonucleases and DNA Ligases. Steps Involved in Recombinant DNA Technology and its Applications in the Production of Recombinant Products like Insulin, Hepatitis B Vaccine, Growth Hormone and Interferon Application of Gel Electrophoresis and Southern Blot Techniques Legislation and Risks Involved in the Use of Recombinant DNA Products. UNIT - IX IMMUNOLOGY Antigens, Structure and Formation of Antibodies, Antigen Antibody Reactions. Diagnostic Tests such as Shickâs Test, Elisa Test (HIV), Widal, Mantoux and VDRL, Methods Involved in Production of Vaccines Polio Myelitis, BCG, Typhoid, Diphtheria and Tetanus Toxoid and Production of Sera Diphtheria Antitoxin. UNIT - X FERMENTATION Introduction to Fermentation Technology, Methods of Production of Pencillin, Streptomycin, Riboflavin and Cyanocobalamine. UNIT - XI MICROBIOLOGICAL ASSAYS Principles and Methods of Different Microbiological Assays Including Sensitivity Testing, Microbiological Assay of Cephelexin, Streptomycin and Vitamin B12. Standardization of Vaccines and Sera. UNIT - XII ANIMAL TISSUE CULTURE Techniques, Nutritional Requirements and Characters of Animal Cell Cultures. Hybridoma Technology, Production and Applications of Monoclonal Antibodies.