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UNIT - I
INTRODUCTION TO THE SCIENCE OF MICROBIOLOGY
Major Division of Microbial World and Relationship among Them.
UNIT - II
CLASSIFICATION OF MICROBES
Classification of Microbes and Study of Bacteria, Fungi, Yeasts, Actinomycetes, Virus,
Rickettsia and Spirochaetes, Study of Mode of Transmission and Treatment of Microbial
Diseases like, Cholera, Typhoid, Tuberculosis, Diphtheria, Tetanus, Syphilis and AIDS.
UNIT - III
NUTRITIONAL REQUIREMENT OF MICROORGANISMS
Nutritional Requirements, Growth and Cultivation of Bacteria and Virus, Study of
Different Important Media Required for the Growth of Aerobic and Anaerobic Bacteria
and Fungi. Differential Media, Enriched Media and Selective Media, Maintenance of
Laboratory Cultures.
UNIT - IV
SELECTION OF BACTERIA
Different Methods used in Isolation and Identification of Bacteria with Emphasis on
Different Staining Techniques and Biochemical Reactions. Counting of Bacteria. Total
and Viable Count. Bacteriological Analysis of Drinking Water.
UNIT - V
STERILIZATION
Detailed Study of Different Methods of Sterilization Including their Merits and Demerits,
Sterilization of Equipments, Validation of Sterilization. Sterilization Methods for all
Pharmaceutical Products. Detailed Study of Sterility Testing of Different Pharmaceutical
Preparations.
UNIT - VI
DISINFECTANTS
Study of Disinfectants, Antiseptics, Fungicidal and Virucidal Agents, Factors Affecting
their Activities and Mechanism of Action, Evaluation of Bactericidal, Bacteriostatic,
Virucidal Activities, Evaluation of Preservatives in Pharmaceutical Preparations.
UNIT - VII
GENETICS
Introduction to Genetics, Phenotypic and Genotypic Changes in Bacteria, Mutations,
Genetic Exchange in Bacteria-Transformation, Transduction and Conjugation.
UNIT - VIII
GENETIC ENGINEERING
Study of Vector, Plasmids, Transposons, Cosmids, Restriction Endonucleases and DNA
Ligases. Steps Involved in Recombinant DNA Technology and its Applications in the
Production of Recombinant Products like Insulin, Hepatitis B Vaccine, Growth Hormone
and Interferon Application of Gel Electrophoresis and Southern Blot Techniques
Legislation and Risks Involved in the Use of Recombinant DNA Products.
UNIT - IX
IMMUNOLOGY
Antigens, Structure and Formation of Antibodies, Antigen Antibody Reactions. Diagnostic
Tests such as Shick’s Test, Elisa Test (HIV), Widal, Mantoux and VDRL, Methods
Involved in Production of Vaccines Polio Myelitis, BCG, Typhoid, Diphtheria and
Tetanus Toxoid and Production of Sera Diphtheria Antitoxin.
UNIT - X
FERMENTATION
Introduction to Fermentation Technology, Methods of Production of Pencillin,
Streptomycin, Riboflavin and Cyanocobalamine.
UNIT - XI
MICROBIOLOGICAL ASSAYS
Principles and Methods of Different Microbiological Assays Including Sensitivity Testing,
Microbiological Assay of Cephelexin, Streptomycin and Vitamin B12. Standardization of
Vaccines and Sera.
UNIT - XII
ANIMAL TISSUE CULTURE
Techniques, Nutritional Requirements and Characters of Animal Cell Cultures.
Hybridoma Technology, Production and Applications of Monoclonal Antibodies.