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RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES KARNATAKA, BANGALORE ANNEXURE II PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION 1. Name of the candidate and DR. VISHAL ANIL NALAWADE address (in Block Letters) DEPT. OF ORTHODONTICS AND DENTOFACIAL ORTHOPAEDICS, BANGALORE INSTITUTE OF DENTAL SCIENCES AND HOSPITAL AND POSTGRADUATE RESEARCH CENTRE, 5/3 HOSUR MAIN ROAD, BANGALORE – 560029. 2. Name of the Institution BANGALORE INSTITUTE OF DENTAL SCIENCES AND POSTGRADUATE HOSPITAL RESEARCH AND CENTRE, BANGALORE – 560 029. 3. Name of study and subject MASTER OF DENTAL SURGERY IN DEPARTMENT OF ORTHODONTICS AND DENTOFACIAL ORTHOPAEDICS 4. Date of Admission 2 MAY 2009 5. Title of the Topic The effect of sandblasting on the retention of orthodontic bands - An in vitro study. 6. BRIEF RESUME OF THE INTENDED WORK: 6.1 Need for the study: Orthodontic band has been in clinical use for more than 100 years. Inspite of the wide spread use of direct bonded brackets and tubes in clinical orthodontics, the conventional band still plays an important role in fixed appliance therapy. Although bonding of orthodontic tubes to the teeth is receiving much current interest in the form of indirect bonding, the majority of buccal attachments are still being cemented 1 using stainless steel bands and conventional cements, considering the force levels in the posterior region. The retention of orthodontic bands to the tooth surface is important to ensure fixed appliance therapy. Numerous agents have been used to retain orthodontic bands. Zinc phosphate cement was developed and introduced as a dental cement in 1878 and serves as a standard with which relatively newer- Polycarboxylate (1968) & Glass ionomer (1971) cements can be compared. Various surface treatments of bands are carried to improve the retention. Sandblasting is one of them. The purpose of the present in vitro study is to measure and compare the forces required to deband orthodontic stainless steel bands with and without sandblasting while using zinc phosphate, polycarboxylate and glass ionomer cements. 6.2 Review of Literature: 1. This study1 evaluated the effect of sandblasting in both laboratory and clinical trials. The aim of the laboratory studies were to determine the effect of sandblasting on the bond strength and survival time of orthodontic bands applied with Ketac-Cem (Ketac-Cem, Espe, Gmbh, Seefeld/Oberbay, BRD).The amount of cement remaining on the bands after debanding was also assessed. Clinically, the effect of sandblasting on the failure rate of first molar orthodontic bands cemented with Ketac-Cem was assessed. Survival time was assessed following simulated mechanical fatigue in a ball mill. The effect of sandblasting on the failure rate of 320 first molar bands cemented in 107 patients was examined in a half-mouth trial. In vitro, sandblasting increased bond strength by 27% (P<0.01) and produced a three-fold increase in the median survival time relative to the untreated sample (P<0.001) in the ball mill experiment. Sandblasting resulted in more cement remaining on the band rather than on the enamel after band removal. In vivo, sandblasting reduced the clinical failure rate of the first molar bands (P<0.001). 2. This study2 was done to evaluate efficacy of sandblasting of bands to increase the bond strength. One hundred fifty extracted and cleaned bovine maxillary incisors were collected. An apicoectomy was performed on each tooth, and the canal and pulp chamber were cleaned out. Round, 6.35mm-diameter disks of stainless steel band material were either left untreated or sandblasted with aluminium oxide. Fifteen 2 untreated disks and 15 sandblasted disks were cemented by each of five methods. 1. Fuji II LC glass ionomer cement, 2. Ketac-cem radiopaque glass ionomer cement, 3. Blend-EZ zinc phosphate band cement, 4. Vitremer Tri-cure glass ionomer cement (chemically cured), 5. Vitremer Tri-Cure glass ionomer cement (light cured). For shear testing, the flat labial surface of each sample was aligned parallel to the testing jig. Shear bond strengths were measured on an Instron 1350 universal testing machine at a rate of 1mm/minute, and the debonding forces were recorded in pounds. Each tooth and corresponding debonded disk were stored together for assessment of the mode of bond failure under a stereomicroscope at 10X magnification. Failures were scored according to the Adhesive Remnant Index (ARI) of Artun and Bergland 6. The results strongly suggested that sandblasting the interior surfaces of orthodontic bands provides a clear clinical advantage when combined with glass ionomer cements. Shear bond strengths increase significantly, and therefore the failure rate of the cemented bands should decrease. 3. This in vitro study3 was conducted to evaluate the force required to cause debanding when zinc phosphate, zinc polycarboxylate and glass ionomer cements are used as the luting agents; and to determine whether sandblasting the inner surface of the orthodontic bands affects the force required to deband. 20 extracted human mandibular third molars with anatomy consistent with a mandibular first molar and free of any sign of demineralization were selected. The teeth were embedded in epoxy resin (special tray forming material, DeTrey) in plastic ice cube trays. The exposed crowns were cleaned with a dental prophylactic paste (Zircate Prophy Paste, LD Caulk) to remove any foreign debris. Stainless steel orthodontic bands (“A” company, San Diego,Calif) with buccal attachments and lingual buttons were fitted, seated and adapted to each molar. Each band was cemented in place with a polycarboxylate cement (Poly-F Cement, DeTrey). Using an Instron testing machine in tensile mode with a crosshead speed of 0.02 inch per minute, the force required to deband the cemented bands was measured in kilograms and recorded in megapascals. The debanded molar teeth were cleaned using a Schure scaler and pumice to remove any remaining cement. The bands were cleaned in an ultrasonic cleaner. The above procedures were repeated, using the same bands and the same 3 teeth, with zinc phosphate(with fluoride, Ormco, Glendora, Calif) and glass ionomer (Band Lok, Reliance Orthodontic Products, Itasca, III) cements. The whole procedure was repeated with sandblasted bands. Glass ionomer cement demonstrated the highest mean force value required to deband both the nonsandblasted and sandblasted orthodontic bands. Sandblasting the inner surface of the bands proved to be a useful method for increasing band retention for all three cements tested. The mean force required to deband was approximately doubled following sandblasting. 4. The aim of the study4 was to evaluate the effect of different roughening methods on band retention. Three different types of cements were used in the study namely Zinc Phosphate, Zinc Polycarboxylate and Glass Ionomer. 15 extracted human maxillary first premolars were selected and were mounted upto cervical line in a block of self curing acrylic. Five optimally sized, preformed stainless steel bands were selected for each tooth. Buttons were welded to the buccal and lingual surfaces of each band. The borders of the bands were marked for identification and were divided into five groups. Group 1 band was used as control, Group 2 band was sandblasted with 50 micron aluminium oxide powder, Group 3 band was sandblasted with 90 micron aluminium oxide, Group 4 band was surface roughened with a tungsten carbide bur and Group 5 band was surface roughened with green stone. The bands were subjected to tensile load testing using a Testometric Migro 500 Universal machine. All four roughening methods demonstrated significantly greater retention than the control group, but there were no statistically significant differences in retention among the four roughening methods. Every roughened band failed at the cementenamel interface, leaving the cement adhering to the band. In the control group, the cement remained on the enamel surface. 5. The aim of this prospective study5 was to compare the retention of micro-etched and untreated first molar orthodontic bands in a randomized, half-mouth trial. Seventynine patients had 304 bands cemented as part of routine fixed appliance therapy. The effect of micro-etching, patient age and gender, operator, molar crossbite, treatment mechanics, and arch on band failure was investigated. Failure rates and survival times were compared for each variable assessed. Micro-etched molar bands showed 4 a significant reduction in clinical failure rate over untreated molar bands and an increase in mean survival time (P<0.001). Of the other variables examined, only the presence of a molar crossbite had any significant effect on band failure (P = 0.004). 6.3 Objectives of the study: 1. To compare the debanding force of orthodontic molar bands cemented using three different luting cements. 2. Effect of in-office sandblasting procedure on the retention of orthodontic bands using different cements. 3. To correlate the effectiveness of the procedure of sandblasting in a clinical situation following the use of plain bands initially. 4. To evaluate the luting surface of the non-sandblasted and sandblasted bands using scanning electron microscopy. 7 MATERIALS AND METHODS: 7.1 Source of data: 30 extracted human mandibular third molars will be collected from Department of Oral and Maxillofacial Surgery, BIDS, Bangalore. Dental cements Zinc Phosphate (Harvard), Polycarboxylate (Densply), Glass Ionomer (GC Fuji) and 30 preformed Stainless steel orthodontic bands (Ortho Organizers) will be used. 7.2 Methods of collection of data (including sampling procedures if any): Part one Nonsandblasted bandsThe sample will consist of 30 extracted mandibular third molars with anatomy 5 consistent with a mandibular first molar and free of demineralization. The collected teeth will be embedded in epoxy resin in plastic ice cube trays. The exposed crowns will be cleaned with dental prophylactic paste to remove debris. Stainless steel orthodontic bands with buccal attachments and lingual buttons will be fitted, seated and adapted to each molar. Each band will be first cemented with polycarboxylate cement. Using an Instron testing machine in tensile mode with crosshead speed of 0.02 inches per minute, the force required to deband the cemented bands will be measured in kilograms and will be recorded in MPa (megapascals). The debanded molar teeth will be cleaned using a ultrasonic scaler (Woodpecker) and pumice to remove any remaining cement. The bands will be further cleaned by placing in an ultrasonic cleaning tank for 20 minutes to facilitate removal of the residual cement from inside the band. The above procedures will be repeated, using the same bands and the same teeth, with zinc phosphate cement and glass ionomer cement. Part two Sandblasted bands- The second part of study will involve measuring the force required to deband when the inside (luting) surface of the orthodontic band has been sandblasted. For sandblasting, the luting surface of each band will be treated with aluminium oxide powder (50 microns) particles directed from the sandblaster (Microetcher ERC, Danville) under 80 psi of air pressure for 15 to 20 seconds, 24 hours prior to the cementation procedure. The procedure of cementation of bands with all the three types of cement and debanding will be repeated in an identical manner as in the first part of the study. One nonsandblasted stainless steel orthodontic band and one sandblasted band will be photographed using a scanning electron electron microscope (SEM) directed at the luting surface of the band. The photographs will be taken at 25 KV at a magnification of 1000X. The luting surface of each stainless steel orthodontic band will be calculated by cutting the band with scissors and then measuring its length and width to the nearest tenth of a millimeter using a Boley gauge. The force required to deband, will be 6 measured on the Instron machine, recorded in kilograms. The surface area and the debanding force will be then used to calculate values of kilograms per square centimeter for each tooth. Multiplying by a conversion factor of 0.0981 will give the force required to deband in megapascals (MPa). 7.3 Does the study require any investigations or interventions to be conducted on patients or other humans or animals? If so, please describe briefly. Not applicable 7.4 Has ethical clearance been obtained from your institution in case of 7.3? Not applicable 8. LIST OF REFERENCES: 1) Millett D T, McCabe J F, Bennett T G, Carter N E, Gordon P H. The effect of sandblasting on the retention of first molar orthodontic bands cemented with glass ionomer cement. Br J Orthod 1995;22:161-169. 2)Miller S, Zernik J H Sandblasting of bands to increase bond strength. J Clin Orthod 1996; 30: 217-222. 3) Wood D P, Paleczny G J, Johnson L N The effect of sandblasting on the retention of orthodontic bands The Angle Orthodontist 1996; 66:207-214. 4) Güray E, Karaman A I Effects of different roughening methods on band retention. J Clin Orthod. 1997;31:361-366. 5) Hodges S J, Gilthorpe M S, Hunt N P The effect of micro-etching on the retention of orthodontic molar bands: a clinical trial. Eur J Orthod 2001; 23:91-97. 7 9. Signature of Candidate 10. Remarks of Guide 11. Name and Designation of (In block letters) 11.1 Guide (Dr.Vishal Anil Nalawade) This is a genuine study, which will be carried out by the postgraduate student under my supervision and guidance Dr. Vinaya S.Pai Professor and Head Department of Orthodontics and Dentofacial Orthopaedics, Bangalore Institute Of Dental Sciences and Hospital, Bangalore. 11.2 Signature 11.3 Co-Guide(if any) Dr. Nandini S. Professor Department of Orthodontics and Dentofacial Orthopaedics, Bangalore Institute Of Dental Sciences and Hospital, Bangalore. 11.4 Signature 11.5 Head of the Department Dr. Vinaya S.Pai Professor and Head Department of Orthodontics and Dentofacial Orthopaedics, Bangalore Institute Of Dental Sciences and Hospital, Bangalore. 11.6 Signature 12. 12.1 Remarks of the Chairman and Principal 12.2 Signature 8