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Course title microbiology : lab
140 micro
Final term 24\5\1431
Name :
Mark:
university no. :
Question # 1 :
 label different parts of this equipment :
(4)
- This equipment is : …(microscope )………
1
Question # 2 :
 True and false : (7)
1. Always wear laboratory coat to protect from stains, chemicals and microbes. ( )
2. You can open the chemical near the fire.(  )
3. Solid media It has agar in it . (  )
4. Sterilization Making things free of microbes. (  )
5. Dry heat sterilization It includes autoclaves . (  )
6. The Hanging Drop Slide shows the bacterial motility. (  )
7. Pure Culture only one type of microorganism growing on the media plate. (  )
Question # 3 :
 Choose the right answer : (7)
1.
…………….…………….is
- Media
nutrients for the growth of microbes .
- test tube
- autoclaves
2. Endospores will be stained ……………… after staining .
- Red or pink
3.
………………
- green
- blue
use to Provide suitable temperature for the growth of organism.
-oven
- needle
- Incubator
4. We can sterilize Benches with ………. :
- Alcohol
- 50% Dettol
- 100% Dettol
5. Gram positive bacteria will stain …………………
- purple.
– red
- green
6. ……….. distinguish one microorganism type from another growing on the same media
.-Minimal media
- selective media
- differential media
7. The organism growing on the media plate is called as ………...
- Culture
- Colony
- media
2
Question # 3 :
 Write the method for simple staining of fungus and algae: (2)
. 1- Take a slide
. 2- Put some material on the slide
3- Add a drop of methylene blue stain and mix it
4- Put a cover slip and study under microscope (40X) .
3
Question # 4 :
- Identify this organism : (5)
The organism :
classification
Kingdom: Monera
Genus: Gloeocapsa
Kingdom: Monera
Genus: Oscillatoria
Kingdom:protista
Genus: Spirogyra sp
Kingdom: Protista
Genus: Clamydomonas sp
4
Kingdom: Myceteae
Genus:Peziza sp
Kingdom: Myceteae
Genus: Rhizopus sp
Kingdom:Protista
Division: Bacillariophyta
Class: Bacillariophyceae
Genus: Daitom sp
Good luck ,,,,,
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