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Course title microbiology : lab 140 micro Final term 24\5\1431 Name : Mark: university no. : Question # 1 : label different parts of this equipment : (4) - This equipment is : …(microscope )……… 1 Question # 2 : True and false : (7) 1. Always wear laboratory coat to protect from stains, chemicals and microbes. ( ) 2. You can open the chemical near the fire.( ) 3. Solid media It has agar in it . ( ) 4. Sterilization Making things free of microbes. ( ) 5. Dry heat sterilization It includes autoclaves . ( ) 6. The Hanging Drop Slide shows the bacterial motility. ( ) 7. Pure Culture only one type of microorganism growing on the media plate. ( ) Question # 3 : Choose the right answer : (7) 1. …………….…………….is - Media nutrients for the growth of microbes . - test tube - autoclaves 2. Endospores will be stained ……………… after staining . - Red or pink 3. ……………… - green - blue use to Provide suitable temperature for the growth of organism. -oven - needle - Incubator 4. We can sterilize Benches with ………. : - Alcohol - 50% Dettol - 100% Dettol 5. Gram positive bacteria will stain ………………… - purple. – red - green 6. ……….. distinguish one microorganism type from another growing on the same media .-Minimal media - selective media - differential media 7. The organism growing on the media plate is called as ………... - Culture - Colony - media 2 Question # 3 : Write the method for simple staining of fungus and algae: (2) . 1- Take a slide . 2- Put some material on the slide 3- Add a drop of methylene blue stain and mix it 4- Put a cover slip and study under microscope (40X) . 3 Question # 4 : - Identify this organism : (5) The organism : classification Kingdom: Monera Genus: Gloeocapsa Kingdom: Monera Genus: Oscillatoria Kingdom:protista Genus: Spirogyra sp Kingdom: Protista Genus: Clamydomonas sp 4 Kingdom: Myceteae Genus:Peziza sp Kingdom: Myceteae Genus: Rhizopus sp Kingdom:Protista Division: Bacillariophyta Class: Bacillariophyceae Genus: Daitom sp Good luck ,,,,, 5