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CRS 2109 INTRODUCTION TO GENETICS 2. INSTRUCTOR(s): Dr. Settumba Mukasa (BSc Agric, MUK, Uganda; MSc, UQ, Australia; PhD, SLU, Sweden) (Senior Lecturer). 3. COURSE TYPE: Core for: B.Sc. Horticulture I/ BSc HMN I/ BSc Agric II Prerequisites: None 4. COURSE STRUCTURE: 2 Credit units: 15 lecture hours (1 contact hour per week for 15 study weeks) and 30 practical/ tutorial hours (equivalent to 1 contact hour per week for 15 study weeks). 5. COURSE DESCRIPTION: Students undertaking this course will be introduced to concepts and applications of genetics in agriculture. Areas to be covered include: Definition of genetics and role of genetics to humanity; Monohybrid inheritance; Dihybrid inheritance; Extension of Mendelian analysis; Genome organisation; Genome variation; Gene mutations and their implications in breeding; Cell division and development; Genetic linkage analysis and genetic maps; Gene structure and expression; Concepts of population genetics; Concepts of quantitative genetics; Introduction to molecular markers and their applications; and Recombinant DNA Technology in crop and animal breeding. This course puts an emphasis on the principles of heredity, genetic problem solving, and their applications in animal and plant breeding. 6. COURSE OBJECTIVES: General objective To equip students with theoretical and technical aspects of genetic analysis for use in classical and molecular breeding and selection systems of crop plants and animals. Specific objectives To provide students with principles and methods used in the study of genetics To enable students understand the current genetic topics and their influence on modern life To provide a foundation for more advanced studies in agricultural research 7. RECOMMENDED REFERENCES FOR READING Griffiths, A.J.F. et al., 2004. (Eds). Introduction to Genetic Analysis (7th. Edn). Freeman. ISBN-10: 0716749394 Hartl, D.L. and Jones, B. 2007. (Eds.) Essential Genetics. Paperback. ISBN: 780763735272. Jones & Bartlett Publishers, USA. Lewin B., 2004. (Ed) Genes VIII. Ed. Pearson Prentice Hall. ISBN-10: 0131439812 Website http://www.bilogylabonline.com 8. COURSE CONTENT, METHODS OF INSTRUCTION, AND TOOLS REQUIRED TOPIC CONTENT METHOD OF INSTRUCTION / Time allocated 1. Introduction Interactive lecture (1 Definition of genetics hr) Role of genetics to humanity History of genetics in plant and animal Tutorial (2 hrs) breeding 2. Monohybrid Lecture (1 hr) Mendel’s monohybrid crosses. inheritance Genes and alleles defined TOOLS/ NEEDED LCD Projector, BB/Chalk. LCD Projector, BB/Chalk 3. Dihybrid inheritance 4. Extension of Mendelian analysis 5. Extension of Mendelian analysis 6. Genome organisation 7. Genome variation 8. Gene mutations and their importance 9. Cell division and development 10. Genetic linkage analysis 11. Concepts of population genetics 12. Concepts of quantitative genetics 13. Gene structure and expression The law of segregation Assignment 1: Probability as applied to genetics Mendelian dihybrid experiments Law of independent assortment Dihybrid test cross Assignment 2: The punnet square Intra-allelic interactions: incomplete and co-dominance Lethal genes, Pleiotropism Penetrance and expressivity Exercises: Mendelian inheritance Multiple alleles Sex linkage in plants and animals Inter-allelic interactions: epistasis Tutorial 1: The chi-square test Definition of a genome Examples of genomes Chromosome structure Tutorial 2: Karyotypes Chromosome mutation Aneuploidy and euploidy Polyploidy and plant breeding Excursion: Genetic variation Gene mutations Types and causes of mutations Transposable elements Practical 1: Mitosis Mitosis and the cell cycle Meiosis, gametogenesis Contrast meiosis and mitosis Sex determination and linkage Practical 2: Meiosis The concept of linkage Recombination and gene linkage Three-point test cross Linkage maps and mapping Tutorial 3: Recombination frequencies Concept of a gene pool The Hardy-Weinberg equilibrium Evolutionary aspects Practical 3: Random genetic drift Quantitative traits Genotype-phenotype relationship Estimating genetic variance Tutorial 4: Heritability estimate Genes at a molecular level; Transcription and Translation Tutorial (2 hrs) Lecture (1 hr) BB/Chalk, Transport (140 Practical -field tour (3 pple) to hrs) MUARIK. Lecture (1 hr) BB/Chalk, Transport (140 Practical -field tour (3 pple) to hrs) MUARIK. Lecture (1 hr) Tutorial/exercises (1 hr) Lecture (1 hr) Practical - laboratory (3 hrs) Lecture (1 hrs) Practical - laboratory (2 hrs) Lecture (1 hr) Tutorial/exercises (2 hrs) Lecture (1 hr) Tutorial (2 hrs) Lecture (1 hr) Tutorial/exercises (2 hrs) Lecture (1 hrs) Practical - laboratory (3 hrs) Lecture (1 hr) Tutorial/exercises (2 hrs) Lecture (1 hr) LCD Projector, BB/Chalk BB/Chalk, Microscopes, Laboratory (70 pple x 2) BB/Chalk, Microscopes and accessories LCD Projector, BB/Chalk. LCD Projector, Lab (70 pple x 2) LCD Projector, BB/Chalk. BB/Chalk, Handouts, Lab materials (70 pple x 2) LCD Projector, BB/Chalk LCD Projector, BB/Chalk 14. Introduction to molecular markers and their application 15. Recombinant DNA Technology 16-17 Gene regulation in eukaryotes Tutorial 5: Gene regulation The basis of molecular makers Types of molecular markers Tutorial 6: Application of molecular markers in breeding Definition of gene cloning Gene cloning procedure Recombinant DNA technology Assignment 4: Applications of recombinant DNA to agriculture Revision Time Final Examination Tutorial/exercises (1 hrs) Lecture (1 hr) Tutorial/exercises (1 hr) Interactive lecture (1 hr) LCD Projector, BB/Chalk Tutorial (1 hr) 9. SUMMARY OF TIME NEEDED: Lectures Tutorials (and assignments) Practicals 10. COURSE ASSESSMENT: Continuous assessment (Quizzes tests): Continuous assessment (Practicals): University Examination: LCD Projector, BB/Chalk 15 (30) hrs 15 hrs 15 hrs Three (3) quizzes and tests arising from tutorials and assignments during semester week 5, 10 and 15. Students will write 3 practical reports 30% Final examination during week 16-17 of the semester 60% 10%