Download CRS 4205 PLANT VIROLOGY AND BACTERIOLOGY

CRS 4205 PLANT VIROLOGY AND BACTERIOLOGY
2. INSTRUCTOR(s):
Dr. Richard Edema (B.Sc (Agric.) MUK, M.Sc, MUK; PhD (Ohio State University)
Dr. Geoffrey Tusiime (B.Sc (Agric.) MUK, M.Sc, MUK; PhD, MUK
3. COURSE TYPE:
Core for B.Sc. Agric. (Crop Science Option)
4. COURSE STRUCTURE
3 Credit units comprising 30 lecture hours (2 contact hours per week for 15 study weeks) and 30 practical hours (1
contact hour per week for 15 study weeks)
5. COURSE DESCRIPTION:
Characteristics and properties of viral plant diseases. The symptoms of disease produced. Principles of transmission,
viral disease detection, diagnosis, epidemiology, management and current nomenclature used. Plant pathogenic
bacteria, the types of disease they cause their identification, culture, classification, transmission infection processes,
and control. Life cycles in relation to disease development, host physiological responses to infection, ecology and
control.
6. COURSE OBJECTIVES:
 To know and apply principles of identification, physical/chemical/genetic characteristics and virus taxonomy
to problems of diagnosis, etiology, epidemiology
 To appreciate the breadth of viruses infecting plants and their economic impact and control
7. RECOMMENDED REFERENCES FOR READING

Mathews’ Plant Virology (4th Edition). Academic Press (2002). By R. Hull.

Fundamentals of Plant Virology. By R.E.F. Mathews.

Plant Pathology (5th Edition). Academic Press (2001). By G.N. Agrios.

Guide to Plant Pathogenic Bacteria (1986). CABI, Kew, Surrey, England. By J. F Bradbury.

Laboratory Guide for Identification of Plant Pathogenic Bacteria. (2001) 3rd Edition. APS Press. St.
Paul, Minnesotta, USA. By Schaad, N. W., Jones, J. B and W. Chun.

Bergey’s Manual of Systematic Bacteriology. Vol 1. (1984). Williams and Wilkins Co. Baltimore. By
Krieg, N. R. and J. G. Holt.
8. COURSE CONTENT, METHODS OF INSTRUCTION AND TOOLS AND REQUIRED
TOPIC
CONTENT
METHOD OF
INSTRUCTION /
Time allocated
1. Introduction
Interactive Lecture
 Brief review of characteristics of plant viruses
(2 hrs)
 Economic importance
 Morphology
 Structure and composition (Protein/Nucleic
acid)
2. Structure of
Lecture (2 hrs)
 The biological function
viral genome
 Coding
 Virus infection and synthesis
 Practicals: Inoculation of plants.
Practical (3 hrs)
Observation of disease symptoms. Assay:
Local lesion assay. Preservation of specimens.
3. Virus
Lecture (2 hrs)

Seed, pollen, soil, water and vegetative
TOOLS/ NEEDED
BB/Chalk,
BB/Chalk
Plant samples
Lab chemicals
BB/Chalk,
transmission


4. Virus movement

5. Detection and
identification of
plant viruses
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6. Plant virus
taxonomy
7. Important viral
diseases and their
control
8. Important viral
diseases and their
control
9.
materials.
Arthropod transmission - types and
characteristics
Vector transmission by organisms other than
arthropods
Translocation and distribution in plants
Lecture (2 hrs)
BB/Chalk,
Symptoms and virus indexing
Cytology and Light Microscopy
Electron Microscopy
Serology/Serological Techniques
Modern biotechnology Techniques
Nomenclature
Classification system
Practicals: Characterization of viruses;
serologic, centrifugals, spectrophotometric plus
molecular analytical methods; DNA based
polymerase chain reaction techniques
 Brief review of types of symptoms
 Physiology of infected plants
Lecture (2 hrs)
BB/Chalk
Lecture (2 hrs)
BB/Chalk,
Practical laboratory (3 hrs)
Enzymes, Antisera
Lecture (2 hrs)
BB/Chalk,

Lecture (2 hr)
BB/Chalk.
Case studies of viral diseases of importance on
key crops of importance to Ugandan (Cassava,
Maize, Sweet-potato and Cowpeas)
 Current control strategies
Field tour to NaCRRI and MUARIK
 Continuous assessment 1
10. Review of
introduction to
bacteriology
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

Bacteria and mankind
Anatomy of the bacterial cell
Functions of different cell components
11. Bacterial
metabolism


Enzymes, energy production and utilisation
Practical: Isolation of bacteria and bacterial
diagnosis
12. Bacterial
growth
 Conditions influencing bacterial growth
 Practical: : Plant inoculation
13. Bacterial
pathogenesis
 The disease cycle
 Mechanical means of bacterial plant attack
 Chemical means of bacterial plant attack
Practical (4 hrs)
Lecture (1 hr)
Transport for 40
BB/Chalk.
Tutorial/exercises
(2 hrs)
Interactive Lectures BB/Chalk.
(2 hrs)
Handouts.
Lectures (2 hrs)
LCD Projector,
BB/Chalk.
Laboratory Practical Plant disease
(4 hrs)
samples
Culture media
Lectures (2 hrs)
LCD Projector,
BB/Chalk
Laboratory Practical Plant disease
(4 hrs)
samples
Culture media
Lectures (2 hrs)
LCD Projector,
BB/Chalk
Laboratory Practical Plant samples
(4 hrs)
Plastic buckets
14: Bacterial plant
diseases and their
management
15. Bacterial plant
diseases and their
management
contd.
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Practical: Plant inoculation
Root and tuber diseases
Vegetable diseases
Grain legume diseases
Interactive lecture
(1 hr)
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Continuous assessment II
Fibre crops diseases
Banana and Plantain diseases
Flower diseases
Interactive lectures LCD Projector,
(2 hrs)
BB/Chalk
Field tour
Examination (1 hr)
Tour to NaCRRI
and MUARIK (4
hrs)
Class assignment
(1 hr)
16-17


LCD Projector,
BB/Chalk
Revision Time
Final Examination
9. SUMMARY OF TIME NEEDED
Lectures and class assignments
Practicals
32 hrs
26 hrs
10. COURSE ASSESSMENT:
Continuous Assessment Test
Laboratory practicals
Final examination
30%
10%
60%
Transport for 40