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Bacteria-Procaryotic Cells Read pages 360 to 372 and answer the following questions. 1. What are prokaryotes? Cells without a true nucleus 2. To which two kingdoms do prokaryotes belong? Archaebacteria & Eubacteria 3. Contrast prokaryotes and eucaryotes in regard to size and explain the reason. Eukaryotes are larger because they need room for the nucleus 4. What are the general characteristics of Eubacteria? Prokaryotic Unicellular Heterotrophic or Autotrophic Reproduce mainly asexually 5. What are cyanobacteria? (Where do they live , What did they used to be called, What is special about them?) 6. What are the characteristics of Archebacteria? 7. Why are archebacteria considered to be different from eubacteria? 8. What are the three basic shapes of bacteria? Rod shaped, spherical and spiral shaped 9. Complete the table: Name of bacteria Bacilli Shape of bacteria Rod shaped Drawing Spherical cocci Spiral shaped spirilli 10. What other methods are used to differentiate bacteria? 11. Who was Hans Christian Gram? He invented the gram stain. 12. Complete this table: Name Gram positive Colour purple Cell membrane Only one cell structure layer outside the cell membrane Gram negative Red Two cell layers outside the cell membrane 13. Describe the different ways that bacteria can move. Some use flagella while others lash, snake, or spiral forward. 14. If bacteria were classified according to their method of obtaining energy, what groups would they be classified into? Phototrophic autotrophs Chemotrophic autotrophs Chemotropic heterotrophs Phototrophic heterotrophs 15. Compare and contrast HETEROTROPHS and AUTOTROPHS. Heterotrophs and Autotrophs are both types of bacteria (ie. Prokaryotic unicellular organisms), they need energy to stay alive while autotrophs are able to make their own organic molecules by harnessing energy from light or chemicals and chemotrophs need to take in organic molecules then breaking them down. 16. Define and give an example of the following: a. obligate aerobes need oxygen to stay alive b. obligate anaerobes are poisoned by oxygen and do not need it to stay alive 17. c. facultative anaerobes Can survive with or without oxygen How often can bacteria reproduce? As often as every 20 minutes 18. Why don’t bacteria reproduce to reach a mass approximately 4000 times the mass of the Earth? Growth of bacteria is held in check by availability of food and the production of waste products. 19. Complete yet another table: Method of Reproduction Sexual or Asexual asexual Binary Fission sexual Conjugation asexual Spore formation Purpose of method of Reproduction To produce many identical offspring To create genetic variety within the species To survive adverse conditions 20. Give some examples of methods of food and beverage production in which bacteria are important. They are used to make cheese, buttermilk, sour cream as well as pickles, kimchi, sauerkraut and some vinegar and wine. 21. Give some examples of how bacteria are used in industry. Digesting oil during an oil spill Removing waste products and poisons from water Help mine minerals from the ground 22. What is symbiosis? A close relationship between two species in which at least one species benefits from the other. 23. What are E. coli? What do they do for us? Bacteria that live in our intestines. They help us digest food and make a number of vitamins. 24. On what does every living thing depend on for growth? A supply of raw material 25. What role do bacteria have in breakdown of dead material? Bacteria recycle and de compose or break down dead material. 26. What are saprophytes? Organisms that use complex molecules of a once-living organism as their source of energy enriching the soil in which it grew. 27. Describe the role of bacteria in sewage decomposition? Bacteria is added to human waste in sewage. They break down the complex compounds in the sewage into simpler compounds. This process produces purified water, nitrogen gas, and CO2 gas and leftover products that can be used as crop fertilizers. (Sometimes it doesn’t work so well and we get lettuce with E. coli on it.) 28. What is that plant called that you smell when you cross the Alex Fraser Bridge? Annacis Island Waste Treatment plant. 29. What % of N2 is our atmosphere made of? 80% 30. What form of nitrogen do living things require? NH4 or NO331. In order for humans to make nitrogen-containing fertilizers they must use the Haber process, the one at 500° C. Describe the Haber process in more detail. Haber process: Take nitrogen gas + hydrogen gas heat the mixture to 500° C and squeeze it to 300 times the normal atmospheric pressure to make NH4. It is expensive and timeconsuming. 32. What do bacteria do to make nitrogen-containing compounds? They live in the roots of legumes and take N2 from the air and change it into NH4 at room temperature and normal pressure. 33. Describe the symbiotic relationship with nitrogen fixing bacteria in soybeans. Soybeans provide food for the bacteria and the bacteria provide organic nitrogen (NH4 )for the plant (mutualism) 34. How much nitrogen is released into the environment every year. More than 170 million metric tonnes per year. Just from those tiny bacteria.