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Chapter 2 Plant Cells and how they work Robert Hook first termed the word cell in 1665 and later went on to describe cell structure. Cells were defined as the basic unit of life in the 1800s. Developments in microscopy have provided much more detailed information since then. Cell Structure *fig 2.3 Walls Cell Wall: Structure that contains all other parts of the plant cell :protoplast Made of cellulose (starch) Primary cell wall: Laid down first and made mostly of cellulose Secondary cell wall: laid down internally to the primary wall Present only in support tissues Made mostly of lignin that provides support….gives wood its strength Pits: openings in the cell walls Plasmodesmata: channels that pass through pits and connect adjacent cells thereby allowing for intercellular transport Middle Lamella: sticky substance found between adjacent cells made of Pectin which is used in making jelly Protoplast All of the plant cell enclosed by the cell wall -nucleus -cytoplasm: organelles, cytosol (fluid component), microtubules and microfilaments: cytoskeleton which lends support and cell shape plasma membrane *fig 2.6: located internal to the cell wall and is made of a phospholipid bilayer with protein channels, intermembrane proteins, glycoproteins etc -semipermeable structure providing regulation of transport in and out of the cell Organelles: membrane bound structures in the cell with specialized functions Chloroplasts: Photosynthesis: CO2 + H2OCH2O (sugar) + O2 -pigment containing disc shaped organelles found in many copies/phosynthetic cell -chlorophyll is the main pigment that gives plants their green color -have their own DNA which is inherited maternally internal structure: Grana: are interconnected stacks where chlorophyll is concentrated Stroma: protein rich fluid outside the grana and inside the membrane Leucoplasts: starch storage structures Chromoplasts: contain various pigments and are abundant in colored plant parts such as flowers and fruits Mitochondrion: Respiration CH2O (sugar) + O2: CO2 + H2O + ATP -break down of sugar to produce the universal energy carrying molecule ATP -membrane bound -many copies/cell -have their own maternally inherited DNA internal structure: -inner and outer membrane -cristae: inner membrane containing proteins of the electron transport chain -matrix: contained within the inner membrane = site of ATP formation Central Vacuole: large membrane enclosed structure containing sap: water based solution of sugar, salt , amino acids, proteins, and crystals. -composes up to 90% of some plant cells -serves as storage of waste as well as source of reserves -anthocyanins which are water soluble pigments are stored in the vacuole Internal Membrane System: Synthesis packaging and transport of substances Endoplasmic reticulum: network of membranes connecting to the nucleus which transports messages from the nucleus to the ribosomes attached to the rough ER Ribosomes: site of protein synthesis -made of RNA and protein Smooth ER: transport and packaging of proteins Golgi Apparatus: storage, modification and packaging of proteins Nucleus: Contains the chromosomes the cell's instructions and the material of inheritance Cell Division Mitosis: nuclear reproduction associated with asexual somatic cell growth -produces 2 daughter cells that are genetically identical to the original mother cell Cell Cycle Interphase: Cell growth and preparation to divide -during interphase the chromosomes replicate(duplicate) *fig 2.9 Prophase: Chromosomes condense and sister chromatids and centromeres can be seen, the nucleus disintegrates Metaphase: Dyads (replicated chromosomes) align on the equatorial plane of the cell, spindle fibers grow from the centrioles and attach to the centromeres of each chromosome or dyad Anaphase: spindle fibers pull in opposite directions thereby separating the sister chromatids and creating a whole new set of chromosomes Telophase: the nuclear membrane begins to reform creating 2 distinct and identical nuclei Cytokinesis: Cell division Osmosis and Diffusion All things in nature seek an equal concentration Diffusion: The movement of molecules across a semipermeable membrane from a region of higher concentration to a region of lower concentration -this process is spontaneous and releases energy in the process -the reason it release energy is that it requires energy to create a gradient (concentration difference between 2 adjacent areas) to begin with. Some of this energy is release as the gradient diffuses Osmosis: The diffusion of water in response to a gradient when the solutes cannot diffuse -if the membrane is not permeable to the solutes involved in the gradient, water will diffuse to equalize concentrations. Hypertonic: solution with a greater solute concentration than within a cell or reference solution Hypotonic: solution with a lower solute concentration than within a cell or reference solution Diffusion and Osmosis are passive transport processes and therefore release energy. Active transport which requires the pumping of molecules against a gradient requires energy. This is normally accomplished with the assistant of protein pumps and uses an energy source such as ATP