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WORD LIST ACETYLMETHYL CARBINOL ACID ACID ACID AEROBIC ALKALINE ALKALINE ALPHA NAPHTHOL AMMONIA ANAEROBIC BILE SALTS BLACK BLUE BROMTHYMOL BLUE BUTT CARBON DIOXIDE CARBON SOURCE CO2 COLORLESS DEXTROSE DEXTROSE DURHAM FERMENTATION EHRLICH’S FLAGELLA GRAM NEGATIVE BACILLI GREEN H2S HYDROGEN SULFIDE IMVIC INDOLE INDOLE LACTOSE MACCONKEY METHYL RED MOTILITY MRVP NEGATIVE NONPATHOGENS PATHOGENS PHENOL RED PINK PINK 2 POTASSIUM HYDROXIDE RED RED RED RED RING SALMON SELECTIVE SEMISOLID SLANT SODIUM CITRATE SPREADING TURBIDITY STAB SUCROSE TRIPLE SUGAR IRON AGAR TRYPTOPHANE TSI UREA UREASE VOGES PROSKAUER YELLOW YELLOW BIOCHEMICAL MEDIA II ACROSS DOWN 1. 1. 2. 6. 9. 11. 14. 15. 17. 19. 20. 22. 24. 26. 28. 31. 33. 34. 35. 36. 39. 41. 44. 45. 46. 48. 50. 52. 53. 55. 57. 58. 59. An agar slant containing 3 sugars, phenol red and ferrous ammonium sulfate. The V in IMViC stands for the ____ _____ test. Oxygen conditions on the TSI slant. The Voges Proskauer reaction is determined by adding two reagents; KOH and ____ _______. Broth used for the methyl red and Voges Proskauer tests. A red to yellow color change on a TSI slant indicates the fermentation of lactose and/or ____. MRVP broth contains the sugar _____. The organisms that produce colorless/yellowish colonies on MacConkey’s agar are generally ____. The lower half of a TSI tube is called the _____. The _____ media is in the form of an agar deep/blunt. A pink urea agar slant is due to the production of an ______ pH. Citrate agar contains _____ _____, agar, and an indicator. The small inverted test tube in the dextrose broth is called the ______ ______ tube. The Voges Proskauer is a test for the production of _____ _______. Indicator in the citrate agar. Bubbles or cracks in the TSI medium indicate the production of ____ gas. Black precipitate in the TSI medium indicates the production of _____ _____. Growth occurring in a straight line in the motility medium indicates a ______ reaction. When inoculating TSI one must ____ the butt. Pathogens growing on MacConkey’s agar appear yellow or ________. Urea agar is used to test for the production of the enzyme ________. A “bubble” occurring in the Durham fermentation tube is due to the production of _____ _____ gas. A yellow color in a carbohydrate broth indicates an _____ pH. Reagents used to determine the indole reaction are ether and ______ reagent. Color that indicates negative MR test. Motility media is used to detect the presence of _______. The “I” in IMViC represents the _____ test. The citrate test determines an organism’s ability to utilize sodium citrate as a ______ source. The motility medium is a gel or ________. Hydrogen sulfide. The oxygen conditions in the butt of a tube of TSI. 3. 4. 5. 7. 8. 10. 11. 12. 13. 16. 18. 21. 23. 25. 27. 29. 30. 32. 33. 37. 38. 40. 42. 43. 47. 49. 50. 51. 54. 56. 2 Indole broth contains the amino acid _______. The indole, methyl red, Voges Proskauer and citrate tests are referred to as the _____ tests. Color that indicates positive methyl red test. MacConkey’s agar contains the sugar _____. A positive urea agar is ____ in color. MacConkey’s will support the growth of the enteric ____ _____ _____ only. A ___ color in TSI is a negative reaction. Color that indicates an alkaline/positive reaction in citrate agar. Urease hydrolyzes urea into ______. A red color produced in a carbohydrate broth indicates a neutral or _____ pH. _____ colored colonies on MacConkey’s agar indicates a positive lactose reaction. Indicator used in the carbohydrate broths. Triple sugar iron agar is also called _____. One of the reagents used to determine the Voges Proskauer test. The methyl red test is a test for the production of ____. Those bacteria that are lactose positive on MacConkey’s agar are generally the _________. A positive motility test is indicated by ____ ____. Indicator used in the MR test. A red to yellow color change produced in carbohydrate is due to ____ production. _____ agar is used to test for the production of the enzyme urease. MacConkey’s agar contains ____ ____ to prevent the growth of the harmless intestinal flora. This medium is used for the isolation of enteric Gram negative bacilli. A yellow color produced in the butt of a TSI is due to the fermentation of _____. A positive indole test is indicated by the observation of a ___ ____ after adding the necessary reagents. MacConkey’s agar is a differential and a ______ medium. Color that indicates a negative citrate test. If tryptophane is hydrolyzed the end products produced are pyruvic acid and _____. The uninoculated urea agar slant is ____ in color. A positive carbohydrate broth is ______ in color. The color that indicates the production of H2S in TSI. In TSI, a yellow ____ indicates Lactose and/or sucrose metabolism. Positive VP reaction is ___ in color.