Download Lab Exam Review Sheet 080711

Lab Exam Review
Suggested use of this list: Study you lab exercises and use this list to test yourself.
NOTE: I am doing this to help you. Not all these topics will be on the test and I may have missed a few
things…..
Be ready to interpret results, describe or perform procedures, identify organisms, etc.
Organisms: know relevant characteristics (gram stain, colony appearance, bacterial morphology, special
properties like hemolysis, spore formation , etc.) and ways to isolate/enrich/confirm their presence
Micrococcus species
Bacillus species
Streptococcus species
Staphylococcus species
Mycobacterium species
Clostridium species
Escherichia coli
Pseudomonas aeruginosa
Steptococcus pyogenes
Staphylococcus aureus
Propionibacteria acnes
Coliform bacteria
Normal skin biota
Media types: complex/rich/chemically-defined, selective/differential, important ingredients
Glucose Salts Agar (GSA)
Trypticase Soy (TS) Agar
Tripticase Soy Yeast (TSY) agar
Eosin Methylene Blue (EMB) Agar
MacConkey agar
Mannitol- Salt agar
Fermentation broths
Shake tube
Bromocresol purple agar
Blood agar
Brilliant Green Lactose Bile Broth
LES Endo Agar
MR-VP broth
Simons Citrate slants
Urea slants
Tryptone broth
Theory/Procedure:
Pure culture
Streak plate technique
Aerobic/Anaerobic culture methods
Aseptic (sterile) technique
Differential stains
Gram stain
Simple stains
Negative stains
Bacterial morphology/arrangement
UV exposure (including molecular changes)
Motility (wet mount & motility deep- no pH indicator)
Hemolysis types
Gram stains
Chemical disinfection
Kirby-Bauer Test for Antibiotic Susceptibility
Transformation experiment (know controls)
Transformation vs conjugation
Parts of Microscope
Plate Count Method of microbial quantitation (determine # organisms/ml)
Serial dilution
Multiple Fermentation Tube Assay
MPN Index
Confirmation test
Completed test
Biochemical tests & Components:
Types of Fermentation (Mixed acid vs Butanediol)
Durham Tube
pH indicator (Note: we use phenol red in fermentation tubes- your results will be different than what the
book describes)
Lactose fermentation
Citrate metabolism
Urea metabolism
Indole production
Kovac’s reagent
Catalase enzyme and test (slide and plate method)
Oxidase enzyme and test
Methyl Red test
Voges-Proskauer (VP) test
Coagulase test