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MITOSIS IN PLANT CELLS Objective: to observe the stages of mitosis. Reproduction of most cells in plants and animals uses mitosis and cell division. In this activity, you will study mitosis in plant cells by examining prepared slides of onion roottip cells. How can plant cells in different stages of mitosis be distinguished from each other? Materials Prepared slide on an onion root tip Microscope Safety: Wash hands thoroughly after the activity. Stage of Mitosis Number of Cells Observed Percent of Cells Observed Prophase Metaphase Anaphase Telophase Total Procedures: (Read all procedures prior to starting the experiment) 1. Obtain a prepared slide of cells from an onion root tip. 2. Set your microscope on low power and examine the onion root tip. Move the slide until you see the tip of the root. You will see several large, round cells. These cells are called the root cap. Move your slide until you see the cells in the area just behind the root cap. Turn the nosepiece to high power. 3. Find one area of cells where you can see most stages of mitosis. Count how many cells you see in each stage of mitosis. _______________________________________________________ 4. Turn the microscope back to low power. Remove the onion root-tip slide. 5. Complete the following statements for your inference: (use complete sentences to answer the questions/10% off final grade if not used) a. Compare the cells in the region behind the root cap to those in the root cap. How are the cells in the region between the onion root tip different from those in the root tip? ___________________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ b. What is the shape of the cells in the onion root tip? ___________________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ c. In which stage do the chromosomes move to the center of the cell and then move to opposite ends of the cells? ___________________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ d. Calculate the percent of cells found in each stage of mitosis. Infer which stage of mitosis takes the longest period of time. ___________________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________
