Download 1b Lab TQ bank Types of media

Lab 1 Test Bank
Types of Media
True or False:
T: All purpose media is a nutrient agar that supports the growth of a wide variety of organisms, but does
not support fastidious organisms.
T: The purpose of Enriched Media is used to grow FASTIDIOUS organisms, such as pathogens.
T: Jams, jellies and preserves do not develop bacteria growth because sugar acts as a bacterial
preservative.
What is an enriched media?
A. A type of media with added nutrients such as vitamins and amino acids.
B. It is used to grow bacteria which are hard to grow, known as FASTIDIOUS organisms.
C. Both A & B
Sabouraud’s Dextrose Agar (SDA)
A: is a media with high sugar content and an acidic pH.
B: SDA isolates.
C: To isolates molds and yeasts which do well in high sugar content, whereas bacteria are inhibited.
D. A & B Only
E. All of the above
What is the difference between Broths and Slants?
A: Broths have no agar.
B: They do not have to be boiled, just stirred.
C: Slants have an agar and they are boiled.
D: After they are removed from the autoclave, they are placed on slant racks to cool so the agar in the
tube stays at a slant.
E: A & B Only
F: All of the above
Why use a slant?
A: One can inoculate the top of a slant to get growth of aerobic bacteria
B: Or we can stab a needle of bacteria in to the tube to see if there are microbes that can grow without air.
C: The tubes last for a long time in the refrigerator
D: The tubes are useful for making pure stock cultures.
E: A & B only
F: All of the above
What does Semi solid allows us to observe?
A: is microbe is motile ?
B: is the microbe is aerobic or anaerobic.
C: Both A & B
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What is an agar?
A. Pathogen
B. Pure culture
C. Seaweed product
D. Bacterial culture
What is agar?
A: Seaweed product in which nutrients are added to, then heated and sterilized for the
purpose of being inoculated to encourage growth of the sample.
B: A part of the cellular structure of algae
C: An abbreviation for accelerated growth activated racemose
D: A & B only
E: All of the above
What is the purpose of Enriched Media?
A. It is used to support a wide variety of organisms.
B. It is used as a bacterial preservative.
C. It is used to grow fastidious organisms, such as pathogens.
D. It is used to isolate molds and yeasts.
What is an example of enriched media?
A. Nutrient Agar
B. Sabouraud’s Dextrose Agar
C. Broths
D. Blood Agar
What is the content of Blood Agar?
A. Trypic Soy Agar and 5% sheep’s blood
B. 40 grams of sugar
C. Trypic Soy Agar and 10% Blood Agar
D. Stock culture
How is culture media classified?
A. By consistency and contents
B. By pH and glucose
C. By aerobic and aerobic bacteria
D. By incubation and inoculation
What is the purpose of a semi-solid consistency?
A. -See if the microbe is motile (can move).
B. -See if the microbe is aerobic or anaerobic.
C. -Isolate molds and yeasts
D. All of the above
E. (a and b)
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What is All Purpose media?
A. A Nutrient Agar that supports fastidious organisms.
B. A Nutrient Agar that has high sugar content and an acidic pH.
C. A Nutrient Agar that supports a wide variety of organisms, except fastidious
organisms (difficult to grow bacteria).
D. None of the above.
How does Sabouraud’s Dextrose Agar sugar (SDA) act as bacterial preservative in a Selective
media?
A. SDA isolates molds and yeasts, which do well in high sugar content, whereas bacteria
is inhibited.
B. SDA provides low sugar content and a pH of 7.0.
C. SDA provides an anaerobic environment.
What is the difference between Nutrient Agar and Broths?
A. Broths have same nutrients as NA, but no agar.
B. No boiling needed, just stirred.
C. Broths are placed in tubes instead of flasks.
D. All of the above
What is the difference between Nutrient Agar and Broths?
A: Broths have same nutrients as NA, but no agar.
B: No boiling needed, just stirred.
C: Broths are placed in tubes instead of flasks.
D: Broths have different nutrients as NA, and have no agar.
E: Boiling is needed, just.
F: Broths are placed in flasks instead of tubes.
G: A, B & C
H: D, E & F
Which statement is true about slants?
A. Slants are poured into petri dishes.
B. Slants are poured into tubes.
C. Slants are made exactly like Soy Agar.
D. Slants are have no agar.
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