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Name BIOLOGY 263, FALL 2006 – RU 01 TUES AM FINAL EXAMINATION Date: Laboratory Practical Stations. (71%, 2% per answer; 180 points, 5 points per answer) 1. a. 10. a. b. b. 2. a. 11. a. b. b. 3. a. 12. a. b. b. 4. a. 13. a. b. b. 5. a. 14. a. b. b. 6. a. 15. a. b. b. 7. a. 16. a. b. b. 8. a. 17. a. b. b. 9. a. 18. a. b. b. Definitions. Define each BIOLOGICAL term from THIS LABORATORY in an accurate, concise, and lucid manner. (7.2%, 2.4% each; 18 points, 6 points each) 1. Bacterial Colony: 2. Electrophoresis: 3. Negative Control Group: Hypothesis Evaluation. Is there statistically significant support for each of the following hypotheses? (6%, 2% each; 15 points, 5 points each) 1. Hypothesis = The species richness of bacteria is greater in fertilized lawns than in unfertilized lawns. Data = Mean number of species on a Petri plate spread with 0.25 ml of a 1:100 unfertilized lawn soil dilution = 5.5, Mean number of colonies on a Petri plate spread with 0.25 ml of a 1:100 fertilized lawn soil dilution = 2.3, p value = 0.012 Circle one: Hypothesis Supported Hypothesis NOT Supported 2. Hypothesis = The density of bacteria is greater in fertilized lawns than in unfertilized lawns. Data = Mean number of colonies on a Petri plate spread with 0.25 ml of a 1:100 unfertilized lawn soil dilution = 16.7, Mean number of colonies on a Petri plate spread with 0.25 ml of a 1:100 fertilized lawn soil dilution = 19.3, p value = 0.064 Circle one: Hypothesis Supported Hypothesis NOT Supported 3. Hypothesis = Plant species richness is higher at higher altitudes. Data = Mean number species of plants around Denver = 27.5, Mean number species of plants around the Eisenhower tunnel (higher in elevation) = 23.1, p value = 0.001 Circle one: Hypothesis Supported Hypothesis NOT Supported Data Analysis and Interpretation. Given the following data from a small, reproductively isolated, constant-sized population, a) calculate the allele frequencies for every time period. (4%, 0.4% each frequency; 10 points, 1 points each frequency) b) Create a properly labeled graph of the allele frequency of one of the alleles (NOT both) over time. (4.8%, 12 points) c) Is the population at Hardy Weinberg equilibrium at time 0? (show calcs.) (4%, 10 points) d) Propose a plausible evolutionary explanation for what you observed based on a)-c)? (2%, 5 points) generations 0 5 10 15 20 individuals BB 16 35 77 87 92 Bb 48 57 20 11 8 bb 36 5 3 2 0