Download Gene Section EIF3F (eukaryotic translation initiation factor 3, subunit F)

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Gene Section
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EIF3F (eukaryotic translation initiation factor 3,
subunit F)
Jiaqi Shi, Mark A Nelson
Department of Surgery, Arizona Cancer Center, University of Arizona, USA (JS), Department of Pathology,
Arizona Cancer Center, University of Arizona, USA (JS, MAN)
Published in Atlas Database: July 2009
Online updated version : http://AtlasGeneticsOncology.org/Genes/EIF3FID44407ch11p15.html
DOI: 10.4267/2042/44774
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 France Licence.
© 2010 Atlas of Genetics and Cytogenetics in Oncology and Haematology
Function
Identity
eIF3f is a subunit of eIF3 complex and is highly
conserved among species. eIF3f interacts with the
caspase-processed isoform of CDK11 (CDK11p46),
which appears to be a down-stream effector in
apoptotic signaling. eIF3f can be phosphorylated at
Ser46 and Thr119 by CDK11p46 during apoptosis. The
phosphorylation of eIF3f contributes to translation
inhibition and apoptosis. The expression of eIF3f is
significantly decreased in pancreatic cancer and
melanoma. Loss of the eIF3f allele has been reported in
pancreatic cancer and melanoma. Ectopic expression of
eIF3f causes rRNA degradation, inhibits translation and
cell proliferation, and induces apoptosis in pancreatic
cancer and melanoma cells. On the other hand,
knockdown of eIF3f prevents apoptosis in pancreatic
cancer and melanoma cells. Recent studies also showed
decreased cell growth, cell proliferation, colony
formation and increased apoptosis in eIF3foverexpressing NIH3T3 cells.
Other names: EIF3S5; eIF-3-epsilon; eIF3-epsilon;
eIF3-p47; eIF3f; eIF3 subunit p47; p47
HGNC (Hugo): EIF3F
Location: 11p15.4
Note: eIF3f is one of at least 13 subunits comprising
the eukaryotic initiation factor 3 (eIF3) complex.
DNA/RNA
Description
10.82kb long.
Transcription
8 exons; transcript length: 3,228 bps; 1073 bp coding
sequence.
Pseudogene
Chromosome 2 from 58,332,079 to 58,333,164 bp
(AC007250.3, Ensembl).
Homology
Protein
eIF3f is not found in S. cerevisiae; However it is found
in Schizosaccharomyces pombe (SPBC4C3.07, 35%
identity) and in Drosophila Melanogaster (CG9769,
49% identity).
Description
357 amino acids; 37,563.75 Da; Isoelectric point:
5.122; Mov34/MPN/PAD1 domain.
Mutations
Expression
Ubiquitous.
Note
No eIF3f mutations have been reported.
Localisation
Cytoplasmic. There is also evidence of its existence in
the nucleus.
Atlas Genet Cytogenet Oncol Haematol. 2010; 14(6)
550
EIF3F (eukaryotic translation initiation factor 3, subunit F)
Shi J, Nelson MA
Implicated in
References
Pancreatic cancer
Shi J, Feng Y, Goulet AC, Vaillancourt RR, Sachs NA, Hershey
JW, Nelson MA. The p34cdc2-related cyclin-dependent kinase
11 interacts with the p47 subunit of eukaryotic initiation factor 3
during apoptosis. J Biol Chem. 2003 Feb 14;278(7):5062-71
Disease
Decreased eIF3f mRNA and protein was found in
pancreatic cancer.
Oncogenesis
Decreased eIF3f gene expression may result from loss
of eIF3f gene allele and down regulation of
transcription. Overexpression of eIF3f in pancreatic
cancer and melanoma cells can lead to 28S rRNA
degradation, decreased translation and increased
apoptosis. On the other hand, knockdown of eIF3f
attenuates apoptosis in tumor cells.
Shi J, Kahle A, Hershey JW, Honchak BM, Warneke JA, Leong
SP, Nelson MA. Decreased expression of eukaryotic initiation
factor 3f deregulates translation and apoptosis in tumor cells.
Oncogene. 2006 Aug 10;25(35):4923-36
Masutani M, Sonenberg N, Yokoyama S, Imataka H.
Reconstitution reveals the functional core of mammalian eIF3.
EMBO J. 2007 Jul 25;26(14):3373-83
Doldan A, Chandramouli A, Shanas R, Bhattacharyya A,
Cunningham JT, Nelson MA, Shi J. Loss of the eukaryotic
initiation factor 3f in pancreatic cancer. Mol Carcinog. 2008
Mar;47(3):235-44
Melanoma
Doldan A, Chandramouli A, Shanas R, Bhattacharyya A,
Leong SP, Nelson MA, Shi J. Loss of the eukaryotic initiation
factor 3f in melanoma. Mol Carcinog. 2008 Oct;47(10):806-13
Disease
Decreased eIF3f mRNA and protein was found in
melanoma.
Oncogenesis
See above.
Iadevaia V, Caldarola S, Tino E, Amaldi F, Loreni F. All
translation elongation factors and the e, f, and h subunits of
translation initiation factor 3 are encoded by 5'-terminal
oligopyrimidine (TOP) mRNAs. RNA. 2008 Sep;14(9):1730-6
Lagirand-Cantaloube J, Offner N, Csibi A, Leibovitch MP,
Batonnet-Pichon S, Tintignac LA, Segura CT, Leibovitch SA.
The initiation factor eIF3-f is a major target for atrogin1/MAFbx
function in skeletal muscle atrophy. EMBO J. 2008 Apr
23;27(8):1266-76
Muscle atrophy
Disease
eIF3f is a key target of MAFbx, an E3 ubiquitin ligase,
during muscle atrophy and has a major role in skeletal
muscle hypertrophy. During muscle atrophy, MAFbx
targets eIF3f for ubiquitination and degradation. Thus,
eIF3f appears to be an attractive therapeutic target.
Kojima KK, Okada N. mRNA retrotransposition coupled with 5'
inversion as a possible source of new genes. Mol Biol Evol.
2009 Jun;26(6):1405-20
Shi J, Hershey JW, Nelson MA. Phosphorylation of the
eukaryotic initiation factor 3f by cyclin-dependent kinase 11
during apoptosis. FEBS Lett. 2009 Mar 18;583(6):971-7
To be noted
Valente ST, Gilmartin GM, Mott C, Falkard B, Goff SP.
Inhibition of HIV-1 replication by eIF3f. Proc Natl Acad Sci U S
A. 2009 Mar 17;106(11):4071-8
Note
eIF3f is a potential tumor suppressor in pancreatic
cancer and melanoma. Loss of heterozygosity and
decreased expression of eIF3f has been found in most
human pancreatic cancer and melanoma specimens.
Silencing of eIF3f increases cell proliferation and
colony formation. Restoration of eIF3f expression
induces cancer cell death.
Atlas Genet Cytogenet Oncol Haematol. 2010; 14(6)
This article should be referenced as such:
Shi J, Nelson MA. EIF3F (eukaryotic translation initiation factor
3, subunit F). Atlas Genet Cytogenet Oncol Haematol. 2010;
14(6):550-551.
551