Download Genetics CTY Course Syllabus Day 1

Genetics
CTY Course Syllabus
Day 1
Morning (9am-12pm)
Time
Topic/Activities
9:00-9:15
Introduction, What is Genetics?
9:45-10:15
Development of Community Standards
- Large sheet of paper to hang in the lab, have TA generate similar
on smaller sheet of paper to make copies. Have students sign
large version – base it in the CTY honor code – respect for self,
respect for others, respect for experiment
9:15-9:45
10:15-10:30
10:30-11:15
11:15-12:00
Student introductions
- 3x5 index cards – maybe have students fill them out without
names, then exchange and play a ‘guess who’ type game?
Break
Pre-test
Review of Cell types and organelle structures
- Slides, perhaps have them do:
o http://www.wisconline.com/Objects/ViewObject.aspx?ID=ap11604
o http://www.cellsalive.com/cells/cell_model.htm
If initial activities take longer than expected, move discussion of cell-types to pm.
Afternoon (1pm-3pm)
Time
Topic/Activities
1:00-1:30
Review of Mitosis and Meiosis
- Slides, including both cartoons and cell micrographs
1:45-2:00
Break
1:30-1:45
2:00-3:00
Introduction to laboratory notebooks and lab reports, Lab Safety
- Make sure each student has a copy of expectations and space in
notebook for lab notes and protocols
Mitosis microscopy – first read Appendix 2-1, 2-2 (p. 77-79)
- REQUIRES MICROSCOPES. Look at 100 cells and determine phase
of mitosis. Use to calculate time in each phase. Before they go,
discuss statistical tests we could use with class data.
Evening (7pm-9pm)
Time
Topic/Activities
7:00-7:45
Lab notebooks and prep reading for Day 2
- Give the TA reign over this part to establish authority. Have TA
evaluate lab notebooks before moving on. Put reading assignment
on slide at the front of the room. (p 27-38, 81-94)
7:45-8:00
8:00-9:00
Day 2
Break
Mitosis human model
- Break students into groups of 8. One group for mitosis, one for
meiosis. See what they come up with! Bring some supplies (paper,
markers, twine). --- clay chromosomes if rain.
Morning (9am-12pm)
Time
Topic/Activities
9:00-10:15
Introduction to Mendelian genetics,
- Diploid nature of organisms, alleles. Monohybrid cross
(Dominance, Segregation), Di-hybrid cross (Independent
assortment). Break into groups to discuss how this could work
given what we know about meiosis.
10:15-10:30 Break
10:30-11:15 Introduction to probability used in genetics
- And/Or statements with genetics examples. M&M Worksheet
11:15-12:00 Introduction to statistics used in genetics
-
Categorical and continuous tests with genetics examples, do some
practice examples, let them design problems and trade.
Afternoon (1pm-3pm)
Time
Topic/Activities
1:00-1:15
Introduction to Laboratory Reports
- Make sure everyone understands expectation and has a copy.
1:30-2:15
Genetics counseling scenarios using probability and chi-square analysis
1:15-1:30
2:15-2:30
Read about pedigrees and make your own for several traits.
- Read 54-64. Draw own pedigree for one trait and evaluate
genotypes
Break
Time
Topic/Activities
2:30-3:00
Write lab reports
If students finish lab reports, they can start reading and problems for evening.
Evening (7pm-9pm)
Time
Topic/Activities
7:00-8:00
Finish lab reports. Read & problems (p. 40-46, 50-54, 211-222)
8:00-8:15
8:15-9:00
Day 3
Break
Human Mendelian Traits Lab Activity.
- Calculate probabilities for future offspring and grandchildren
Morning (9am-12pm)
Time
Topic/Activities
9:00-9:15
Review of lab reports
9:15-10:00
Case Studies of Mendellian Extensions
- Pleiotropy Lethality, Expressivity, Epistasis
10:00-10:30 Slides to discuss extensions of Mendel
-
10:30-10:45 Break
Allelism, Co-dominance, incomplete dominance, lethality
10:45-12:00 Methods to evaluate extensions:
Linkage, Sex-linkage, Complementarity, Do problems as a group
Afternoon (1pm-3pm)
Time
Topic/Activities
1:00-1:15
Introduce Lab Set-up
- http://learn.genetics.utah.edu/content/begin/traits/blood/
2:00-2:15
Break
1:15-2:00
Blood Typing Lab (w/ Lab Report)
- Wards 36V-0041
Time
Topic/Activities
2:15-3:00
Lab Reports + Online Lab
- http://www.phschool.com/science/biology_place/labbench/lab7/in
tro.html
- http://www2.edc.org/weblabs/SexDetermination/sexdetermination
.html
- http://vgl.umb.edu/
Evening (7pm-9pm)
Time
Topic/Activities
7:00-8:00
Read and problems on linkage/sex-linkage
- p 94-101, 121-137
8:00-8:15
8:15-9:00
Break
Read papers on Sex-determination
Online sex linkage lab if extra time.
Day 4
Morning (9am-12pm)
Time
Topic/Activities
9:00-9:15
Review of lab reports, problems
9:15-10:00
Introduction to chromosomal structure
- What is a chromosome? Contrast proks with euks. Nucleosome
structure, heterochromatin vs. euchromatin. How do they fit in the
nucleus?
10:00-10:15 Break
10:15-11:00 Closer look at what chromosomes are
-
DNA structure, chromosome structure. What types of information do
they contain? (MAP)? What are alleles and how do they arise?
11:00-12:00 Read about recombination and allele exchange. Discuss when and how this
occurs
Afternoon (1pm-3pm)
Time
Topic/Activities
1:00-2:00
Techniques to identify chromosomes
- Linkage maps vs structural maps, Banding patterns, FISH, size, location
of centromere
Time
Topic/Activities
2:00-2:15
Break
2:15-3:00
Practice with karyotyping
- Use paper copies for diagnosis. Cut and align
- http://www.biologycorner.com/karyotype/index.html
Evening (7pm-9pm)
Time
Topic/Activities
7:00-8:00
Chromosome theater – allow students to develop 5 minute ‘plays’ on
changing chromosome dynamics.
8:00-8:15
8:15-9:00
Day 5
Break
Read and problems (p. 137-152), review for quiz
Morning (9am-12pm)
Time
Topic/Activities
9:00-9:15
Review of problems and/or concerns for eval.
9:15-10:00
10:00-10:15
10:15-10:45
10:45-12:00
Quiz
Break
Discussion: Review of where we have been this week. Set up with ‘what are
genes’? Re-introduce molecular processes and central dogma. If two people
have the same genes, then they should be the same right? Why or why not?
10:45-12:00Videos on epigenetics + exploration of learn@utah site on
epigenetics.
Videos on epigenetics and exploration of learn@utah site on epigenetics.
Discuss reasons (epigenetics, pleiotropy, expressivity) why people with
same alleles don’t always have same traits to set up next week’s discussion
of molecular genetics.
Afternoon (1pm-3pm)
Time
Topic/Activities
1:00-2:00
Reebops?:
- http://kvhs.nbed.nb.ca/gallant/biology/Mystery_at_the_Termond_E
state.html
2:00-3:00
Disease Research Activity??? Should have all terms necessary to describe
various diseases of choice.
Sunday Evening (7pm-9pm)
Time
Topic/Activities
7:00-8:00
Read Chapter 1 (p. 1-24)
8:00-8:15
8:15-9:00
Day 6
Break
Read p.256-261, Build DNA model out of random supplies, making sure to
differentiate between strong bonds and weak bonds.
Morning (9am-12pm)
Time
Topic/Activities
9:00-9:15
Introduction to Molecular Basis of Genetics and potential applications
10:00-10:15
Break
9:15-10:00
10:15-11:00
11:00-11:30
11:30-12:00
DNA as the genetic material
- How to approach a scientific paper. Read papers that lead to our
understanding.
Student presentations on papers (plan ~5 minutes each) to discuss main
points
Mechanism of DNA replication in prokaryotes and eukaryotes. Importance of
fidelity. Video of human model??
Lab Prep and reading
Afternoon (1pm-3pm)
Time
Topic/Activities
1:00-2:00
DNA Extraction lab.
2:00-3:00
Macromolecule Test & Clean-up
Evening (7pm-9pm)
Time
Topic/Activities
7:00-8:00
Write complete laboratory reports
8:00-8:15
8:15-9:00
Break
Read p.266-276, 283-297
Day 7
Morning (9am-12pm)
Time
Topic/Activities
9:00-9:15
Review of homework, l
9:30-10:15
Mechanism of Transcription
9:15-9:30
Discussion of the purpose of DNA, what would happen if mistakes are made
during replication
10:15-10:30 Break
10:30-11:15 Post-transcriptional processing
11:15-12:00 Mechanism of Translation
Afternoon (1pm-3pm)
Time
Topic/Activities
1:00-1:30
Codon Game, Translation Worksheet
1:30-3:00
RNA Module Activity 1
Evening (7pm-9pm)
Time
Topic/Activities
7:00-8:00
Outcomes of mutation, introduction to gene expression
- Diseases of expression
8:00-8:15
8:15-9:00
Day 8
Break
Read 381-397, problems
Morning (9am-12pm)
Time
Topic/Activities
9:00-9:15
Review of homework
10:15-10:30
Break
9:15-10:15
10:30-11:00
11:00-12:00
What are mutations, how are they developed? How are they prevented?
Which are detrimental to organism? Which to offspring?
Telephone Game
Lac operon regulation, extension to arabinose operon
Afternoon (1pm-3pm)
Time
Topic/Activities
1:00-1:15
Introduction to electrophoresis
1:15-1:45
1:45-2:00
2:00-3:00
Pour and run gels
Break
Lac Operon lab (Carolina 17-1025)
- Make sure to start with predictions based on lecture. Measure the Xgal reaction.
Evening (7pm-9pm)
Time
Topic/Activities
7:00-7:45
Lac operon lab report
7:45-8:00
8:00-9:00
Day 9
Break
Read 169-172, 187-197, problems
Morning (9am-12pm)
Time
Topic/Activities
9:00-9:15
Review of homework
10:15-10:30
Break
9:15-10:15
10:30-11:00
11:00-12:00
Bacterial genetics – idea of gene transfer, types of gene transfer identifiable
mutations
Pre-lab for Transduction
Transduction lab (Carolina 21-1128C)
Afternoon (1pm-3pm)
Time
Topic/Activities
1:00-1:30
Pre-lab for Transformation (Edvotek 222)
1:30-2:15
2:15-2:30
2:30-3:00
Transformation Lab
Break
Pre-lab for Conjugation lab
Evening (7pm-9pm)
Time
Topic/Activities
7:00-8:00
Problem catch-up and review for quiz 2
8:00-8:15
8:15-9:00
Day 10
Break
p. 173-192, problems
Morning (9am-12pm)
Time
Topic/Activities
9:00-9:15
Review of homework and problems
9:15-10:00
Quiz 2
10:00-10:15 Break
10:30-12:00 Conjugation lab and plasmid mapping
Afternoon (1pm-3pm)
Time
Topic/Activities
1:00-2:00
Regulation of Eukaryotic Genes by cis/trans elements
2:00-3:00
Regulation of Eukaryotic Genes by chromatin
Sunday Evening (7pm-9pm)
Time
Topic/Activities
7:00-8:00
Collect remaining data, Write Lab reports
8:00-8:15
8:15-9:00
Day 11
Break
Read p. 415-420, 426-435, 444-446
Morning (9am-12pm)
Time
Topic/Activities
9:00-9:15
Review of homework, and problems
9:15-10:00
Examples of geneXP regulation (development, stem cells maybe, etc)
10:00-10:15 Break
10:30-12:00 Introduction to molecular genetics and techniques, probes
Afternoon (1pm-3pm)
Time
Topic/Activities
1:00-2:00
DNA Fingerprinting Lab, Sequence analysis (Wards 36V 6054)
2:00-3:00
Additional tools in molecular genetics
- http://www.hhmi.org/biointeractive/vlabs/bacterial_id/index.html
Evening (7pm-9pm)
Time
Topic/Activities
7:00-8:00
Collect and analyze data
8:00-8:15
8:15-9:00
Day 12
Break
Discuss DNA sequencing technology and blotting
Morning (9am-12pm)
Time
Topic/Activities
9:00-9:15
Review of homework and problems
9:15-10:00
10:00-10:30
10:30-10:45
10:45-12:00
Review of evolution in general
Brainstorm about role that genetics plays in evolution
Break
Hardy-Weinberg equilibrium and chi-square to evaluate if a population is
actively evolving.
Afternoon (1pm-3pm)
Time
Topic/Activities
1:00-2:00
Population genetics activity
2:00-3:00
Online lab - http://faculty.etsu.edu/jonestc/Virtualecology.htm
Evening (7pm-9pm)
Time
Topic/Activities
7:00-8:00
Read p. 637-654
8:00-8:15
8:15-9:00
Break
Practice problems in H-W
Day 13
Morning (9am-12pm)
Time
Topic/Activities
9:00-9:15
Review of homework and problems
9:15-10:00
Complex Traits – understanding heritability, narrow and broad sense
heritability
10:00-10:30 Practice problems in heritability
10:30-10:45 Break
10:45-12:00 Read 685-707 and practice problems.
Afternoon (1pm-3pm)
Time
Topic/Activities
1:00-2:00
Introduction to understanding genetics as a system
2:00-3:00
Introduction to genomics
Evening (7pm-9pm)
Time
Topic/Activities
7:00-9:00
GATTACA
Day 14
Morning (9am-12pm)
Time
Topic/Activities
9:00-10:00
GATTACA discussion
10:00-10:30 Lab Clean-up
10:30-12:00 ELSI Case studies
Afternoon (1pm-3pm)
Time
Topic/Activities
1:00-2:00
ELSI Presentations
2:00-3:00
Review of course materials
Day 15
Morning (9am-12pm)
Time
Topic/Activities
9:00-9:30
Final Review
9:30-10:30
10:30-12:00
Final Evaluation
Closing Ceremonies