Download T400-Series Surgical Protocol Rat Ascending Aorta: Chronic Blood Flow Measurement Volume Flow

Volume Flow
T400-Series Surgical Protocol
Rat Ascending Aorta: Chronic Blood Flow Measurement
(Right Thoracotomy-Back Exit Probe)
APPLICATION BASICS
Site:
Ascending Aorta
Species:Rat
Body Weight:
325-400 grams
Duration: Chronic
Vessel Diameter:
2.8 mm
PROBE Size:
3 mm
Reflector:
L with sliding cover
Connector:
4-pin
Cable Length:
12 cm
Catalog #:
MC-3PSB-LS-WC12-CM4S-GC
FLOWMETER 3PSB Probe
TS420 Perivascular Module
Right Thoracotomy Using Back Exit Flowprobe
ACKNOWLEDGEMENT
Proper stabilization and orientation of the Probe cable is
essential to keep the Probe in position on the ascending aorta
and to avoid the Probe twisting on the vessel during closure and
exteriorization of the connector to the back.
R. Allan Buchholz, Ph.D., Pfizer Central
Research Div., Groton, CT and
R.L. Dundore, Ph.D., Sterling Drug Co.,
Dept. of Pharmacology, Rensselaer, NY.
Anesthetize the rat with sodium pentobarbital (50 mg/kg
i.p.) and ventilate mechanically. Perform a right thoracotomy
between the second and third ribs. Isolate the ascending aorta
and clear of adipose and connective tissue. Position a 3 mm
PS-Series back cable exit Probe around the aorta as close to
the heart as possible. Pack the space between the body of the
Probe and the aorta with surgical sponge (Mericel). Pass the
cable from the Probe through the thoracotomy and tunnel it
subcutaneously to the top of the skull.
REFERENCES
Dundore RL, Habeeb PG, Pratt PF,
Becker LT, Clas DF, Buchholz RA,
“Differential Hemodynamic Responses to
Selective Inhibitors of Cyclic Nucleotide
Phosphodiesterases in Conscious Rats,” J
of Cardiovasc Pharm 1992; 19: 937-44.
Close the thoracotomy, suture the muscle layers and reduce
the pneumothorax. Affix the Probe connector to the top of the
skull with a skin button (AAPC104) and dental acrylic. Suture
the wounds and treat with a topical antibiotic. Administer a
suspension containing ampicillin (10 mg) subcutaneously.
RL-18b-sp Rev C 2-13
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