Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Volume Flow T400-Series Surgical Protocol Rat Ascending Aorta: Chronic Blood Flow Measurement (Right Thoracotomy-Back Exit Probe) APPLICATION BASICS Site: Ascending Aorta Species:Rat Body Weight: 325-400 grams Duration: Chronic Vessel Diameter: 2.8 mm PROBE Size: 3 mm Reflector: L with sliding cover Connector: 4-pin Cable Length: 12 cm Catalog #: MC-3PSB-LS-WC12-CM4S-GC FLOWMETER 3PSB Probe TS420 Perivascular Module Right Thoracotomy Using Back Exit Flowprobe ACKNOWLEDGEMENT Proper stabilization and orientation of the Probe cable is essential to keep the Probe in position on the ascending aorta and to avoid the Probe twisting on the vessel during closure and exteriorization of the connector to the back. R. Allan Buchholz, Ph.D., Pfizer Central Research Div., Groton, CT and R.L. Dundore, Ph.D., Sterling Drug Co., Dept. of Pharmacology, Rensselaer, NY. Anesthetize the rat with sodium pentobarbital (50 mg/kg i.p.) and ventilate mechanically. Perform a right thoracotomy between the second and third ribs. Isolate the ascending aorta and clear of adipose and connective tissue. Position a 3 mm PS-Series back cable exit Probe around the aorta as close to the heart as possible. Pack the space between the body of the Probe and the aorta with surgical sponge (Mericel). Pass the cable from the Probe through the thoracotomy and tunnel it subcutaneously to the top of the skull. REFERENCES Dundore RL, Habeeb PG, Pratt PF, Becker LT, Clas DF, Buchholz RA, “Differential Hemodynamic Responses to Selective Inhibitors of Cyclic Nucleotide Phosphodiesterases in Conscious Rats,” J of Cardiovasc Pharm 1992; 19: 937-44. Close the thoracotomy, suture the muscle layers and reduce the pneumothorax. Affix the Probe connector to the top of the skull with a skin button (AAPC104) and dental acrylic. Suture the wounds and treat with a topical antibiotic. Administer a suspension containing ampicillin (10 mg) subcutaneously. RL-18b-sp Rev C 2-13 www.transonic.com