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UWS ANIMAL CARE AND ETHICS COMMITTEE Animal Ethics Checklist What is happening to the animals? What will be the effects? How will the effects be minimised? Have you addressed all the relevant topics below in your application? Anaesthesia Euthanasia Fasting Induction & drug, dose, route Maintenance & drug, dose, route Methods of monitoring anaesthesia and recovery Additional support during anaesthesia and recovery (eg heat, intravenous fluids) Location of induction and recovery areas Method Location (where procedure will be performed) Expertise of personnel Behaviour modification Genetic manipulation Stimulus (type, duration, frequency) Methods Potential effects Blood/body fluid collection Housing Volume Route Frequency Anaesthesia or analgesia Restraint Animal monitoring (methods, frequency) Location Isolation Group housing (stocking rates, sexes) Shelter Bedding Hiding areas Environmental enrichment Duration held Conditioning period Diet/water modifications In-vitro studies Type Amount Effects Measurement of intake Animal monitoring Source of animals Duration held Euthanasia Drug treatments Surgery Substance Volume Route Frequency/total number per animal Local and systemic effects Anaesthesia or analgesia Possible side effects Restraint Anaesthesia Location of pre-operative preparation area Pre-operative preparation Surgical procedure (site, technique) Sterile technique (instruments, drapes, surgeon) Location of and housing in post-operative recovery area Post-operative management Post-operative monitoring (methods, frequency, duration) Use of analgesics (type, dose, route, frequency, means of determining necessity for use) Expertise ACEC Application Checklist Aug 14 Toxicology Teaching Substance Volume Route Frequency of treatments / total number per animal Source of animals Housing Duration held Method of disposal Transport Wildlife studies Type Duration Confinement Number of animals Air conditioning Location Methods Capture methods Handling/restraint Housing Monitoring Release Effects on population Tumour/neoplasia induction Method Site Endpoint Animal monitoring (methods, frequency) Reference: ARRP Guideline 12 Updated February 2000 ACEC Application Checklist Aug 14