Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
THE UNIVERSITY OF NEW ENGLAND ANIMAL ETHICS COMMITTEE (AEC) STANDARD OPERATING PROCEDURES FORM (For Domestic Fowl, Native Fauna/Wildlife, Domestic Livestock & Laboratory Animals) Title of Procedure: Use of Tritium in Domestic Fowls (F5) Objective: To determine body water and fat in broiler chickens. Details of Procedures: Birds are starved and denied access to water for 12 hours, then weighed and if they are to be injected more that once a week, a blood sample (minimum, 1ml; maximum, depends on body weight) taken to determine background tritium level. Tritium is then injected, intramuscularly, into the thigh. Each bird is injected with 10 uCi/kg body weight. A stock solution is diluted so that each bird received the required dose in 1ml of solution. Three hours after injections the bodyweight of each bird is recorded and a blood sample taken (1-5ml, depending on bodyweight). Birds are then allowed access to feed and water. For birds up to 28 days of age, blood samples are taken from the jugular vein, there after from a wing vein. Disposable 5ml plastic syringes, fitted with 21 gauge, 38mm needles are used for injection and sampling. Syringes and needles are rinsed with lithium heparin prior to sampling. Blood is transferred to 5ml plastic lithium heparinised vials for storage and/or isotope recovery and counting. Drug, Chemicals or Biological Agents: Sterile tritium/tritiated water. Care of Animals after the Procedure: Return to normal environment. Qualifications, Experience, Skills or Training Necessary to Perform this Procedure: Operators must be familiar with the techniques involved in handling domestic fowls as well as the methods of aseptic blood collection and intravenous drug administration in birds. Thorough knowledge of the techniques and risks associated with use of radioisotopes is required. Effects of Procedure on Wellbeing of Animals: Should be negligible. Pain Relief Measures: Nil References: Nil Prepared by: Dr G Jones (Department of Biochemistry, Microbiology and Nutrition, UNE) and Ruth Tremont (UNE Director of Animal Welfare), 1992 Reviewed by: A/Prof Brian CHEETHAM (Science and Technology), 2006/7