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0.008 0.006 0.004 0.002 0.000 Probability 0.010 0.012 0.014 Fragment Length Distribution 200 400 600 800 Fragment Length Mode = 186, Mean = 207.1, and Std = 73.7 1000 1.0 0.8 0.6 Probability 1.2 1.4 Read Length Distribution 50 60 70 80 90 Read Length Mode = 75, Mean = 75.0, and Std = 0.0 100 0.04 0.02 0.00 Probability 0.06 0.08 Read Start Position Distribution 1 2 3 4 5 6 7 8 9 10 11 Bin # 12 13 14 15 16 17 18 19 20 ● A C G T ● ● 15 ● ● 5 10 ● 0 Observed Quality 20 25 Observed Quality vs. Phred Quality Score ● 5 10 15 20 Phred Quality Score 25 30 35 1e+07 Alignment statistics 8e+06 Unique 39% 6e+06 Unalignable 13% 2e+06 4e+06 Filtered 0% 0e+00 Number of reads Multi 48% 0 2 4 6 8 11 14 17 20 23 26 29 32 Number of alignments per read 35 38 41 Inf