Download Lab 6a-2 Connective Tissue

Lab Exercise 6a-2
Connective Tissue
Nervous
Muscle
Classification of connective
tissues
Connective Tissue
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Connective tissue proper
Fluid connective tissue
Supportive connecting tissue
Connective tissues
Connective tissue proper
 Loose connective tissue
 Areolar
 Adipose
 Reticular
 Dense connective tissue
 Dense regular
 Dense irregular
 Elastic tissue
Fluid CT
Blood
Supporting CTs
Cartilage
Hyaline cartilage
Elastic cartilage
Fibrocartilage
•
Bone
Areolar tissue
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A loose CTP
Areolar
Areolar: what to look for
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Fibroblasts
Collagen fibers
Elastic fibers
Mast cells and macrophages
Found? Throughout body, under dermis,
divides skin from underlying tissues
Fibroblasts
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Resting fibroblasts typically have so little
cytoplasm that the cells appear, by light
microscopy, as "naked" nuclei
Fibroblast
Adipose tissue
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Another lose CTP (note nucleus)
Adipose: what to look for
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Lots of cytoplasm
Slim nuclei pushed off the side
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Found? You know where
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Reticular tissue
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The third type of loose CTP
Reticular tissue
Reticular: what to look for
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Reticular fibers (network)
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Found? Internal framework in many sort
organs (liver, spleen) supporting the
parenchyma
Dense CTP
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Dense regular – strength in one direction
Dense irregular – strength in all directions
Elastic tissue - pliable
Dense regular
Dense regular: what to look for
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Thick parallel bundles of collagen
Small fibroblasts in between bundles
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Found? Tendons, ligaments, deep fascia.
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Dense irregular
More dense irregular
Dense irregular: what to look
for
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Mesh of collagen fibers (irregular looking)
Interspersed fibroblasts
Found? Dermis of skin, periosteum,
perichondrium
Elastic tissue
Elastic tissue: what to look for
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Elastic fibers (instead of collagen fibers) in
large bundles
Fibroblasts
Found? Between vertebrae, in blood vessel
walls (underneath endothelium)
Fluid CT
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Blood
Blood: what to look for
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RBCs
White blood cells (darker): monocytes,
lymphocytes, granulocytes
Platelets
Supportive CT
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Cartilage – gelatinous, padding
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Hyaline cartilage
Elastic cartilage
Fibrocartilage
Hyaline cartilage
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Glasslike because fibers not visible
More hyaline
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There are collagenous and elastic fibers lying
in the cartilage matrix but they are invisible
because their “refractive index” is the same
as that of the matrix (like cornea)
More hyaline
Hyaline cartilage
Hyaline
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Hyaline cartilage (lavender matrix), with
perichondrium (pink) outside it. The latter is a
dense regular collagenous CT. Cartilage cells
= chondrocytes, and they are lying in the
lacunae.
Hyaline cart.: what to look for
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Chondrocytes and lacunae
No visible fibers
Where? Most joints, nasal septum
Elastic cartilage
Elastic Cartilage
Elastic cart: what to look for
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Many elastic fibers in matrix
Chondrocytes in lacunae
May be stacked up
Fibrocartilage
Fibrocartilage: what to look for
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Irregular, wispy collagen fibers
Chondrocytes
Found? Intervertabral discs of spine, pads in
knee joint
Supportive CT: Bone
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Detail of lacuna, showing radiating canaliculi. Tissue fluid from
the capillaries and connective tissue of the Haversian canal can
seep through these spaces and channels, bringing nutrients to
the stellate osteocytes residing there.
Bone: what to look for
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Osteon (whole circular structure)
Concentric lamellae (of matrix)
Central canal (at center of lamellae)
Osteoblasts
Osteocytes in lacunae
Canaliculi
Found? Bones!
Nervous tissue
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Neuron smear
Large, pyramidal cell bodies
Long processes extending out
Nervous Tissue
Figure 4.10
3 Types of Muscle Tissue
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Skeletal muscle:
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Cardiac muscle:
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large body muscles responsible for movement
found only in the heart
Smooth muscle:
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found in walls of hollow, contracting organs (blood
vessels; urinary bladder; respiratory, digestive and
reproductive tracts)
Muscle Tissue: Skeletal
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Long, cylindrical, multinucleate cells with
obvious striations
Found in skeletal muscles that attach to
bones or skin
Muscle Tissue: Skeletal
Figure 4.11a
Muscle Tissue: Cardiac
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Branching, striated, uninucleate cells
interlocking at intercalated discs
Muscle Tissue: Cardiac
Figure 4.11b
Muscle Tissue: Smooth
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Sheets of spindle-shaped cells with central
nuclei that have no striations
Found in the walls of hollow organs
Muscle Tissue: Smooth
Figure 4.11c
Exercises
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Look at all slides
Draw an example of each tissue on paper provided
11 connective tissues:
6 CTP (3 loose, 3 dense)
 1 Fluid CT (blood)
 4 Supportive CT (3 cartilage, 1 bone)
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Neurons
3 Muscle tissues
Skeletal
Striated
Smooth
Turn in on Thurs 10/25
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7 drawings from 6a-1 Epithelia
15 Drawings from 6a-2 Connective+
Review sheet for lab 6a