Download BIOL 260 Spring 2011 Lab Schedule

Spring 2011 Lab Schedule (As always, possibility of modifications)
Date
Apr 5,7
Apr 12, 14
Lab #
1
2
Tues
Lab Introduction
Plating- aseptic technique +
thumbprint
Surface structure/cell walls;
reverse staining
Growth- temp, UV, antibiotic
(tet, strep, vanco, amp)
Apr 19, 21
3
Apr 26, 28
4
May 3, 5
5
May 10, 12
May 17, 19
6
7
May 24, 26
8
Growth- differential selection;
motility (gelatinase)
Transformation- pGLO
Fungi observation (growth on
food started Apr 21)
Bacteriophage
May 31 June 2
June 7, 9
9
10
Wastewater field trip
ELISA (Immuno Explorer?)
Thur
Microscopy tutorial
Staining-Heat vs.
methanol; endospore
Pond water- hanging
drop
Antiseptic and
disinfectant;
Growth assessment
Growth assessment
Protein purification
Bacteriophage
assessment
BIOL260 Lab Schedule Spring 2011
Week 1 How to create a Laboratory Notebook/Fundamentals of Microscopy
Week 2- Septic vs. aseptic techniques in plating/growing bacteria/Staining and
visualization of bacteria and endospores
Week 3- Investigating cell walls and surface structures of
microorganisms/Observations of pond water
Week 4- Investigating microbial growth: differential selection and motility
Week 5- Investigating microbial growth: effects of temperature, ultraviolet
radiation, antiseptics, and antibiotics
Week 6- Bacterial transformation and selection/ protein purification
Week 7- Observations of fungi: molds and yeasts
Week 8- Making bacteria sick: bacteriophage
Week 9- Investigating microorganisms in real-life scenarios-field trip to Waste
Treatment Plant
Week 10- Investigating healthcare diagnostic tools-ELISA