Download qBR4-2a

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

page
1
page
2
page
3
page
4
page
5
page
6
page
7
page
8
page
9
page
10
page
11
page
12
page
13
page
14
page
15
page
16
page
17
page
18
page
19
page
20
page
21
page
22
page
23
page
24
Document related concepts
no text concepts found
Transcript 
Penggunaan CSSLs untuk pencarian
lokasi gen dalam kromosom
Bogor, 21 Februari 2013
?
Apakah CSSLs itu?
?
?
Strategi dasar pemetaan QTL (lokus sifat kuantitatif)
Fenotipe: kerapatan trikoma daun
Populasi: RILs (recombinant inbred lines)
Mauricio, Nature Reviews Genetics, 2001
Metode pemetaan QTL (lokus sifat kuantitatif)
Fenotipe: kerapatan trikoma daun
Populasi: F2
Mauricio, Nature Reviews Genetics, 2001
Pemetaan awal mencari lokasi Istana Bogor
Pemetaan halus mencari lokasi Istana Bogor
Tahap-tahap berurutan dalam kloning gen-gen QTL
Semua tahap ini makan banyak waktu dan tenaga
Ashikari & Matsuoka, Trends in Plant Science, 2006
Produksi Introgression Lines (ILs) atau CSSLs
Ashikari & Matsuoka, Trends in Plant Science, 2006
Chromosome Segment Substitution Lines (CSSLs)
Ebitani et al., Breeding Science , 2005
Pemetaan QTL menggunakan Introgression Lines (ILs) atau CSSLs
Paran & Zamir, Trends in Genetics, 2003
Introgression lines of L. pennellii in cultivated tomato for QTL mapping
FM = fruit mass
Left: a fruit from the wild tomato
species L. pimpinellifolium
Right: a fruit from L. esculentum
cv. Giant Red, bred to
produce extremely large
tomatoes
Eshed & Zamir, Genetics, 1995
Figure 2 High-resolution mapping of the fw2.2 QTL.
A Frary et al. Science 2000;289:85-88
Published by AAAS
Phenotypic effect of the fw2.2 transgene in L. esculentum
pCLD04541
Fruit from R1 progeny of fw107 segregating for
the presence (+) or absence (-) of cos50
• The exact molecular function of fw2.2 is not known, although it shares
homology with the human RAS oncogenes in protein structure and contains
two predicted transmembrane-spanning domains.
• fw2.2 acts as a negative regulator of cell division in the fruit, perhaps
via some role in cell-to-cell communication.
A Frary et al. Science 2000;289:85-88
Published by AAAS
Positions of QTLs for field resistance to rice blast in Japanese upland rice
(r2=45.7%)
(r2=29.4%)
qBR4-2
146 F4 lines (NipponbarexOwarihatamochi)
111 RFLP markers
pi21, a recessive gene encoding a proline-rich protein
(Fukuoka et al., Science, 2009)
Fukuoka & Okuno, TAG, 2001
Pengembangan bahan tanaman Sub-CSSLs
Fukuoka et al., TAG, 2012
Graphical genotypes dari Sub-CSSLs sekitar qBR4-2
Fukuoka et al., TAG, 2012
Graphical genotypes dari Sub-CSSLs sekitar qBR4-2a dan qBR4-2b
Fukuoka et al., TAG, 2012
Pyramiding qBR4-2a, qBR4-2b, dan qBR4-2b
Fukuoka et al., TAG, 2012
Perbandingan sekuen qBR4-2a antara Nipponbare dan Owarihatamochi
Fukuoka et al., TAG, 2012
Peranan protein NBS-LRR dalam ketahanan tanaman terhadap patogen
Spoel & Dong, Nat Rev Immunology, 2012
Analisis filogenetik qBR4-2a dan 30 gen R yang lain
Fukuoka et al., TAG, 2012
Terima kasih
Terima kasih
Related documents