Download Understanding Of Phenol Coefficient Test

Technology And Engineering
Expertise Interest
Chemical analysis
Analysis Of Applied Microbiology
SMK Negeri 13 Bandung
Desinfectant Potence Test
(Phenol coefficient)
SMK Negeri 13 Bandung
Learning Objectives
 After learning process, the students are
expected to be able to:
 1. Descript the basic theory of
desinfectant potency test ( Phenol
coefficient)
2. Work admittedly, accurately, aseptic,
sterile and having responsibility and care
to vicinity area.
 3. Draw up equipment and media which
applied for determination of phenol
coefficient.
 4. Do determination of coefficient phenol.
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Learning Objectives
5. Do the incubation process of
coefficient phenol test
6. Observe the result of incubation of
phenol coefficient.
7. Do calculation phenol coefficient.
8. Report the observation result of
phenol coefficient test
Teknologi dan Rekayasa
Understanding of Disinfectant
Disinfectant is a material, usually
chemistry matter which kill
vegetative cell but is not guaranteed
to kill form of microorganism spores
cause of disease.
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Classification of Desinfectant
 Faction of Phenol
 Faction of Halogen
 Faction of Alcohol
 Faction of Peroxide
 Faction of Heavy metal
 Faction of Aldehyde
 Faction Quaternary Ammonium
 Faction of Gas
Teknologi dan Rekayasa
The Ideal Characteristics Of
Desinfectant

Antimicrobial Activity, at low concentration must
have antimicrobial activity with wide spectrum.

Solubility, must be soluble in water or other solvent
until level needed to be able to applied effectively.

Stability, change happened at substance if it let a
few days must as minimum as possible and may not
eliminate its character manifestly.

Do not have the character of poison

Homogeneous

It is not joined forces with organic material
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The Ideal Characteristics Of
Desinfectant
 Antimicrobial Activity at room
temperature
 Do not generate rust and colour
 Ability deodorize which less delicate
 Have ability as detergent/cleaner h
 Be available in big amounts with
proper price
Teknologi dan Rekayasa
Understanding Phenol coefficient
Phenol coefficient is fractional
number which refers to the strength
comparison of energy power kills
from desinfectant compared to
strength of energy power kills from
phenol as comparator in the same
condition, that is the same bacterium
type and the same residence time.
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Understanding Of Phenol
Coefficient Test
Phenol coefficient test is comparing
antimicrobial activity from chemical
components with phenol as standard
test. Diluting of desinfectant step by
step and phenol, placed in saving
sterile. Bacterium pure culture
applied as standard, added in each
tube. The test bacterium is entered in
each tube with time interval 5, 10,
and 15 minutes. All subcultures
brooded at temperature 370C during
48 hours and seen its turbidity.
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Equipments & Materials
 Broth breeding containing S.aureus ( 24
hours)
 Phenol ( carbolic acid)
 Sterile aquades
 Desinfectant lisol ( sample)
 Fairish steril pipette of 5 mL and 1 mL.
 Tube TSB ( Trypticase Soy Broth) or
NIOBIUM ( Nutrient Broth)
 Small and big test tube
 Tube rack
 Volumetric flask 100 mL
 Ose
 Spiritus Burner
 Stopwatch
 Incubator
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Procedures
 Prepare microbe suspension test.
 Make standard solution of Phenol 5% ( vol/vol)
quantitative.
 Make Sample solution 5% ( vol/vol) quantitative.
 Make various concentration of phenol standard 1:80
1:90 1:100
 Make various concentration of sample 1:80 1:100
1:120 1:140 1:160 1:180 and 1:200
 Do examination of coefficient phenol according to
activity schema.
 Incubation at temperature 37°C during 48 hours.
 Observe the result of incubation and calculate the
price of its the phenol coefficient.
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Activity Schema
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Example Of Observation Data
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Example of Calculation
 The result gotten then is packed into
calculation as follows:
 Desinfectant test at diluting 1 : 450,
showing there is no growth at minute 10th
but grows at minute 5th. Diluting of phenol
at 1 : 90 showing there is no growth at
minute 10th but grows at minute5th.
 Phenol coefficient for desinfectant is :
1 : 450/1 : 90
 Phenol coefficient less than 1 indicates that
the desinfectant less effectively is compared
to phenol. Phenol coefficient more than 1
indicates that desinfectant is more
effectively than phenols.
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Teknologi dan Rekayasa