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1- First line of defense: Plant perimeter protection 2- Second line of defense: Chemical warfare - Terpenes Classes of plant defenses PHYSICAL DEFENCES •Spines, thorns •Cutins, waxes, suberins SECONDARY DEFENCES • Terpenes Physical Defenses Stem spines Colletia paradoxa Leaf spines- Opuntia invicta Shoot spines- Dovyalis caffra Otherwise known as kei apple Drought tolerant Why did spines often evolve in areas that are dry or in other ways “stressful”? Other roles - competition, camouflage? Hydrophobic: having water-repelling properties These compounds are non-polar Fatty acids are one type of hydrophobic compound composed of long fatty acid chains a major component of plant cuticle Plants’ cuticles often vary with the climate in which they live. Cactus cuticle Cactus cuticle complex mixtures of long-chain lipids that are extremely hydrophobic. are synthesized by epidermal cells. exuded through pores in the epidermal cell wall by an unknown mechanism. Also formed from fatty acids but has a different structure from cutin. A cell wall constituent. often within roots. can protect against pathogens and other damage. older parts of roots more suberized endodermis has suberin side walls, water must pass through plasma membrane to get to stele •protect primary metabolism by deterring herbivores, reduce tissue loss. •also attract pollinators and seed-dispersing animals. •formed from the byproducts or intermediates of primary metabolism Secondary Defences Secondary defence may be in place prior to an organism invading a plant, or as a result of the invading organism •constituents of essential oils •function as herbivore deterrents •can be produced in response to herbivore feeding, and to attract predatory insects and parasites of the feeding herbivore. Terpene functions 1.Growth and development 2. As defensive compounds •Toxins •feeding deterrents to insects and mammals Non-volatile terpenes - limonene apparently distasteful to herbivores Volatile terpenes such as menthol broadcast a smell that warns herbivores that the plant is toxic to them before herbivore feeding commences. Phytoecdysones are plant steroids (within the terpene class) that have the same basic structure as insect molting hormones and thus interfere with molting. These compounds sometimes cause death of the insect herbivore. Terpenes such as pyrethrum (from chrysanthemums) and azadirachtin (from the Asian and African Neem tree) can be used as “natural” insecticides in agricultural practices or in horticulture.