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Plant Cells & Tissues Plant Cells Plant Cells have a few unique organelles: Vacuoles Plastids (e.g., chloroplasts) Cell wall Vacuoles Definition: membranous sacs filled with water that serve a variety of functions: Storage of “food”, water, oil, pigments, toxins, & waste). Helps to maintain turgor pressure. Young plant cells often contain many small vacuoles, but as the cells mature, these unite to form a large central vacuole. Plasmolysis Definition: the contraction of cells within plants due to the loss of water through osmosis. It is when the cell membrane “shrinks away” from the cell wall and the vacuole collapses. Occurs when water is drawn out of the cell (high water concentration low water concentration). Causes plants to wilt. Plastids Definition: large organelles that are associated with pigments and other storage products (e.g., starch). A typical plant cell will usually have 20-40 plastids. Several Types: Chloroplasts Amyloplasts/Leucoplasts Chromoplasts Chloroplasts Definition: plastids that capture light energy from the sun to make “food”; contain chlorophyll (Elodea) Amyloplasts Definition: colorless plastids that store starch Leucoplasts are pretty similar. Found in things like potatoes & onions Chromoplasts Definition: plastids that make & store carotenoid pigments Found in red & yellow peppers, tomatoes, flower petals … When leaves change color in autumn, it is the result of the conversion of chloroplasts into chromoplasts. Cell Wall Definition: a flexible outer barrier made of cellulose fibrils that surrounds plant cells. Functions: Protection & Support Determines plant shape & size Maintains turgor pressure Regulates movement in and out Cell-cell communication Plasmodesmata Definition: small tubes that connect plant cells to each other, establishing living bridges between cells. Allow certain molecules to pass directly from one cell to another. These channels, penetrate the cell wall and are lined with plasma membrane, uniting all connected cells with essentially one continuous cell membrane. Stomata Definition: pores found in the underside of plant leaves in the epidermis that allow for the exchange of gases (CO2) and H20. The stomata are flanked by two half moon shaped guard cells, which contains chloroplasts and regulates the opening and closing of the stoma. Normally stomata open when the light strikes the leaf in the morning and close during the night. Pea leaf stoma (SEM x 3, 520) Trichomes Definition: epidermal hairs or scales found on nearly all plants. Functions: Glandular secretions Absorb nutrients Protect against predators Reduce evaporation Reflect solar radiation Collect water Cell Division The mechanism that allows the nuclei of cells to split and provide each daughter cell with a complete set of chromosomes during cellular division. Permits plants to grow & develop. Garlic Root Tip In plants cell division occurs in tissues called meristems, which are often found in root tips. Apical meristem Root cap Phases of Cell Division Interphase Mitosis: G1 phase S phase G2 phase Prophase Metaphase Anaphase Telophase Cytokinesis Interphase Cellular growth Preparation for mitosis Mitosis Prophase Appearance of stringlike chromosomes due to condensing of chromatin. Metaphase Chromosomes line up in the middle of the cell (equator) Anaphase Chromosomes separate and move to opposite ends of the cell (poles). Telophase & Cytokinesis Two new nuclei are formed and the cytoplasm is beginning to separate. Tissues Plant Tissues Definition: a group of cells that forms a structural and functional unit. Three tissue systems in plants: Ground tissue: variety of functions including support and photosynthesis Vascular tissue: “Plumbing system” throughout plants; conducts water, food (CHO), and dissolved minerals; also strengthens and supports the plant. Dermal tissue: Provides covering for the plant body. SIMPLE TISSUES The Ground Tissue System Parenchyma cells Definition: a simple tissue composed of spherical-shaped cells; most common type of tissue (most of apples & potatoes); found throughout plant Have thin primary walls Living, metabolizing tissue Functions: Photosynthesis & Respiration Storage (starch, oil droplets, water, and salts) Wound healing & regeneration Collenchyma Definition: a simple tissue found beneath the epidermis in young stems and in large veins of leaves (“strings” in celery). Provides a flexible support system (like our skeletal system). Found uniformly throughout the plant. Elongated cells that have unevenly thickened cell walls (especially in the corners) Alive at maturity Sclerenchyma Definition: a simple tissue specialized for structural support; occurs in many areas Two types: Fibers Sclereids Have both primary and secondary cell walls. At functional maturity cells are often dead. Fibers Long, tapered cells that often occur in groups or clumps (abundant in the wood and bark of flowering plants) Found in leaves & stems. Primary function is support. These sclerenchyma fibers clearly show the thick cell walls. Notice the pits (identified by the arrows) which allow materials to pass from one fiber to another Sclereids Short, cuboidal cells common in shells of nuts and pits of fruits (peaches, cherries) Found in all plant parts, usually clustered. Primary function is for protection. The red cells in this picture are sclereids from a pear. Notice the irregular shape. The surrounding cells are parenchyma cells. COMPLEX TISSUES The Vascular Tissue System Two Complex Tissues Xylem Phloem Both are continuous throughout the plant body. Xylem Conducts water and dissolved minerals from the roots and the stems and leaves & provides support. Composed of four types of cells: Tracheids* Vessel elements* Parenchyma cells Fiber (*Both of these conduct water!) Tracheids Tracheids are typically found in gymnosperms and seedless vascular plants and aid in transporting water. The hollow cells that are stained red are tracheids from a fern. Vessel Elements Vessel elements are typically found in angiosperms and aid in transporting water. Upper right is a long section of vessel elements showing the perforation plate Lower right is a cross section of vessel elements. Phloem Conducts food materials, carbohydrates, in solution and provides support. Composed of four different cells: Sieve-tube members (carry food) Companion cells (aid STM) Schlerenchyma fibers (support) Parenchyma cells (storage) Sieve-tube members (STM) Long, thin cells that are stacked endto-end to form extended sieve tubes. Some of the most-specialized cells in nature that are alive, but lose their organelles at maturity. The end of the cells are called sieve plates. Adjacent to STM are companion cells, which assist in the STM by moving food into the sieve-tube for transport. Sieve-tube Members (STM) This is a sieve tube member from milkweed. Notice how STM’s are connected by sieve plates (identified by the arrows). Sieve-tube Members (STM) This picture show several sieve tube member in cross section. Notice the perforated sieve plate near the top that would connect two sieve tube members. Sieve-tube Members (STM) Sieve tube members can secrete a protein called callose to plug the seive plates and stop the flow of food if there is damage in the phloem. This picture shows an example. Companion Cells Companion Cells are alive and regulate the movement of material through sieve tubes. The arrows point out several companion cells adjacent to the sieve tube members. Notice the sieve plate as well. Dermal Tissue System Dermis In herbaceous plants the dermis is a single layer of cells called the epidermis. Epidermis Epidermis Comprised of two types of cells: Parenchyma cells (storage) Guard cells (together create stoma) Epidermal parenchyma contain no chloroplasts, so they appear transparent allowing light to penetrate into interior photosynthetic tissues. This picture shows the single layer of parenchyma cells that makes up the epidermis and also a very thick cuticle. Epidermis Functions: Absorption of water Secretion of cuticle Protection Control of gas exchange Epidermis--Cuticle This picture shows the single layer of parenchyma cells that makes up the epidermis and also a very thick cuticle. Cuticle Parenchyma cells Cuticle A waxy layer that is secreted by epidermal cells of stems and leaves. Functions to prevent water loss. Also prevents important gases like CO2 from diffusing into leaf/stem. Epidermis—Guard Cells This picture shows the single layer of parenchyma cells that makes up the epidermis along with the cuticle and a pair of guard cells creating a stoma. Epidermis—Guard Cells This picture shows the parenchyma cells that makes up the epidermis and a couple pairs of guard cells (and stomata) from the top. Stomata & Guard Cells Stomata are tiny pores in the epidermis between two cells called guard cells. Many gases, including CO2, O2 and H2O vapor, pass through stomata by diffusion. Stomata are generally open during the day during photosythesis. The water lost when stoma are open elps to cool the leaf! Usually closed at night to conserve water. Peridermis Woody plants initially produce an epidermis that splits as the plant grows. These plants also have periderm, which is several cells thick and forms under the epidermis. Periderm composes the outer bark. Peridermis Composed of two types of cells: Cork Cells that secrete suberin to help waterproof the plant. Cork Parenchyma that function for storage. Peridermis from an ivy stem. Plants Exhibit Localized Growth at Meristems Meristems Definition: specific areas of plant growth, which are composed of cells that do not differentiate. These cells retain the ability to divide, which other plant cells lose. Meristems allow plants to grow their entire lives. Types: Apical Lateral Intercalary Types of Growth Primary growth: an increase in the length of a plant. All plants have primary growth. Secondary growth: an increase in girth of a plant. Only select plants (gymnosperms and woody dicots) have extensive secondary growth. Wood and bark are produced by secondary growth. Types of Meristematic Tissues Apical Meristems Found at or near tips of roots and the buds of stems. Cells are small and “boxy” in shape due to parenchyma cells. Responsible for the increase in length of roots and stems. Root tips have a root cap at the tip of the apical meristem, which is followed by an area of cell elongation and an area of cell differentiation. Stem buds have a dome-like apical meristem in addition to leaf primordia and bud primordia, which protect the apical meristem. Lateral meristems Definition: areas that extend the entire lengths of stems and roots except at tips, which are responsible for secondary growth in trees & shrubs. Two types: Vascular cambium —a layer of cells that forms a thin cylinder around the stem and root trunk, between the wood and bark. Cork cambium —a thin cylnder of cells in the outer bark region. Intercalary meristems Growth regions that occur at the base of nodes. Found in many plants that don’t have a vascular cambium (grasses).