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Download Cultivation of plant viruses Protoplast and callus method
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Cultivation To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication. Cultivation Animal Bacteria Plant Insect Methods of animal virus cultivation 1. Organized tissue. 2. Cell culture. 3. Laboratory animals. Chick embryo technique Methods of animal virus cultivation 1. Organized tissue. 2. Cell culture. 3. Laboratory animals. Cell culture 1. 2. 3. 4. 5. 6. 7. 8. Removal. Homogenization. Washing. Trypsinization. Washing. Growing. Inoculation. Incubation. Methods of animal virus cultivation 1. Organized tissue. 2. Cell culture. 3. Laboratory animals. Intracerebrally Intraperitoneally Intramuscularly Intravenously Intranasally Types of cell culture Primary Suspension Diploid Permanent Primary cell culture A culture derived directly from a tissue Diploid cell culture Subsequent cultures derived from primary cell culture. Suspension cell culture A culture in it the dispersed cells are not allowed to settle down, but they are kept floating by continuous shaking of the flasks. Permanent cell line A culture derived from single separated cell i.e. diploid culture it can be propagated indefinitely. Cultivation of plant viruses • Protoplast and callus method. • Mechanical method. • Biological method. Cultivation of plant viruses • Protoplast and callus method. • Mechanical method. • Biological method. Inoculation Incubation Inoculation Incubation Cultivation of plant viruses • Protoplast and callus method. • Mechanical method. • Biological method. Cultivation of plant viruses • Protoplast and callus method. • Mechanical method. • Biological method. Incubation Cultivation of bacterial viruses 1. On broth media 2. On solid medium Virus + semisolid agar Bacteria + solid agar Cultivation of Insect viruses Criteria of purity Chemical purity Physical purity Biological purity
