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BSC 197: Molecular and
Cellular Basis of Life Lab
TA info
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Drinie Clairardin
[email protected]
Office/Lab: SLB 245/247
http://biology197.wikispaces.com/
Lab Info
• Lab schedule can be found on the syllabus
– Course website: www.bio.ilstu.edu/bsc197
• Lab will consist of an exercise one day
and a wet lab the following day
Lab Safety
• Safety is the number one priority
• ABSOLUTELY NO food, drink or tobacco in Lab
• Lab safety equipment available and will be used as
needed (eg. Goggles, gloves)
• Location of Eyewash, fire extinguisher
• Do not endanger yourself or others
• Respect everyone in lab
Assignments
• All lab reports must be typed (including graphs)
• Do the lab report as follows
– Intro: Short background about the lab; include
hypothesis
– Materials and Methods: Summarize briefly; focus on
the reasons, don’t need exact details
– Results: tables/graphs of data and text to summarize
what they show
– Discussion: analysis of results; troubleshoot lab if it
didn’t work
– Conclusion: short summary of everything
• All lab reports are due at the beginning of the
lab, two days after the lab was completed
Plagiarism Policy
• Plagiarism will not be tolerated
– Consequences of being caught for plagiarism
include receiving a zero for the class and
being referred to student affairs for
disciplinary action
• Do your own work
– Make your own graphs
– Write your own papers
– Cite sources
Exercise 1
• We will use several types of graphs in the
lab
– Bar (listed as column graph in excel): good
choice when only using a few data points; can
have words in the x-axis; x-axis is not evenly
numbered
Graphs (cont’d)
• Line Graph: used when you are looking at
a change over time; x-axis not drawn to
scale and x-axis can have letters
Graphs (cont’d)
• Scatter (XY Scatter): X-axis drawn to
scale; data will be plotted in its exact
position not just at the labeled point; can
not be used if there are letters needed on
the x-axis
Graphs (cont’d)
• Pie chart: Used when describing a part or
percentage of a whole
Graphs (cont’d)
• Graph requirements:
– X and Y axis titles, labels and units
– Graph title (must be descriptive)
– Legend
– Failure to include ALL requirements will result
in point deductions
Statistical Calculations
• Pages 11-14 (top portion-not including T-Test)
• Mean- Average

X 1  X 2  X 3  X 4  X 5...
MEAN 
N
•N = total number of replicates
•X1, X2, X3… Individual data Points
Mean Calculation Example
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X1 = 79
X2 = 85
X3 = 92
X4 = 87
X5 = 93
X6 = 99
• (79 + 85 + 92 + 87 + 93 + 99)=535
• 535/6=89.17
Statistical Calculations (cont’d)
• Standard Deviation (S.D.)- measures variability of
the mean
S.D. 
N *(X )  (X )
N ( N  1)
2
2
•N- number of replicates
•(X2)- Sum of the squares of X
•(X)2- The square of the sum of the values for X
Standard Deviation (S.D.) Example
Calculation
Larva #
X
Larva #
X
X2
1
0.124
1
0.124
0.015376
2
0.11
2
0.11
0.0121
3
0.131
3
0.131
0.017161
4
0.099
4
0.099
0.009801
5
0.119
5
0.119
0.014161
N=5
Sum of X = 0.583
Sum of X2 = 0.068599
Square X
values
5(0.068599) - ((0.583) 2 )
S.D. 
5(5  1)
S.D.= 0.012461942
Statistical Calculations (cont’d)
• Standard Error (S.E.): estimates reliability of the
mean
( S.D.)
S . E. 
(N )
(0.012461942)
S . E. 
5
S.E.= 0.005573149 = 0.006 (rounded off)
Statistical Calculations (cont’d)
• Do not round values until the end
• Show all your work for the calculations
For tomorrow:
• Exercise 1 due
• Lab 1: The Properties of Enzymes
– Read Lab 1 in the manual
• Dress appropriately
– No open toed shoes or shorts above the
knee!