Download Fingerswitch for Bipolar Forceps - Computer

TEAM MEMBERS
Dan Carlson
Dave Ugai
Erik Bieging
Team Leader
Communicator
BWIG/BSAC
“THE SOLUTIONATORS”
From left: Dave Ugai, Erik Bieging, and Dan Carlson
CLIENTS
Jinling Wang, Ph.D.
Research Associate
Department of Physiology
Gail A. Robertson, Ph.D.
Associate Professor
Department of Physiology
ADVISOR
Professor John G. Webster, Ph.D.
ABSTRACT
The objective of the project is to design an
automated system of valves for a solution
delivery system to be used in patch clamp
research. The current system has two to eight
solution reservoirs suspended in the air above
a manifold. The manifold allows the
researcher to manually control which solution
is being delivered. This is a tedious process
for the researcher, so an automated system is
desired.
PROBLEM STATEMENT
The goal of this project was to
develop a computer-automated
system for delivering solutions to a
patch clamp for physiological
laboratory research.
PATCH CLAMP



Method used to study
single cells
HERG potassium
channel is critical in
repolarization of the
heart’s action potential
Long QT syndrome is a
disease that can lead to
cardiac arrhythmia or
sudden death
MICRO PIPET OVER ION CHANNEL
http://www.iac-usnc.org/Methods/wholecell/equipment.html
CURRENT SETUP
Solutions are fed
to a manifold and
as the orientation
of the multiposition stop-cocks
is changed, the
solution being
delivered changes
MOTIVATION
•The current method for switching
solutions is manually changing the
alignment of valves on a manifold
•This is a tedious process that
wastes valuable research time
CLIENT REQUIREMENTS
 Must
reliably open and close valves on
command from the computer
 Compatible with up to eight feeds of
medical tubing
 Must generate minimal electrical and
mechanical noise (so as not to disturb
delicate physiological research)
VARIABLE DESIGN
COMPONENTS
1.
Valves: solenoid or solenoid pinch
valve (both normally closed)
2.
Valve Controller: Valvelink8,
Controlled 8 relay driver, Manually
assembled controller
SOLENOID VALVES
When current runs through the solenoid the
plunger is drawn up and the valve opens
SOLENOID PINCH VALVES
The spring loaded plunger pinches the tubing, and
when current runs through the solenoid, the plunger
is pulled back.
DECISION MATRIX
Price
Valvelink8 R85PRO Manually
assembled
1
5
9
Simplicity 10
8
4
Reliability 10
9
7
Total
24
20
21
FINAL DESIGN COMPONENTS
1. R85PRO relay driver (1)
2. 1/8 inch solenoid valves, normally closed (8)
3. RSIO Adapter (1)
4. Serial cable (1)
5. 8-into-1 Teflon micro manifold with flow control
(1)
6. NPT Male Pipe adapter (1/8” x 1/16”) (16)
MOUNTED DESIGN
1. R85PRO Driver
3
2. Solenoid valves
1
2
3. RSIO adapter
The design was
mounted on foam
pads in a plastic
box to reduce the
mechanical noise
produced by the
valves
FUTURE WORK
 Install
device in client’s laboratory
 Configure
with pClamp data acquisition
software
 Provide
instruction to client on usage
REFERENCES





Jack Commins, sales representative, AutoMate
Scientific
L. Burke O’Neal, Biomedical Engineering Instrument
Innovator, UW Madison
Ryan Sheldon, owner, National Control Devices,
controlanything.com
James A. Sullivan, author, www.cellsalive.com
American Heart Association, website,
www.americanheart.org