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Things to Do Today • Observe Urinalysis Plates – Note hemolysis & Count Colonies on BAP – Observe EMB plate • • • • Blood Lecture Blood Typing Differential WBC Count Observe Parasitic Blood Slides Whole Blood • Plasma • Buffy coat • Hematocrit RBCs RBC Disorders • Anemias -hemolytic -Iron deficiency -aplastic -sickle cell Polycythemia WBCs Wikipedia Commons, used with permission WBCs neutrophil eosinophil basophil monocyte Wiley lymphocyte WBCs WebPath, used with permission WBCs lymphocyte Medicine USC, used with permission Vs. monocyte Leukocyte Disorders • Leukemia • Infectious mononucleosis • Leukopenia Formation of platelets Clotting Blood Types Hemolytic DZ of the Newborn • Mom must be Rh (-) • Father must be Rh (+) • A child must be Rh (+) • A subsequent child must be Rh (+) Hemolytic DZ of the Newborn Blood typing Watch demo of Blood Smear 10 sec in dye 20-24 sec in rinse Blood Parasites Microbe Disease • Trypanosoma cruzi Chaga’s disease • Leishmania Leishmaniasis • Plasmodium vivax Malaria • Trypanosoma gambiense Sleeping sickness Trypanosoma cruzi 1000x Etiological Agent for Chaga’s disease KM Rhodnius: Vector for Chaga’s disease KM AKA assassin bug; kissing bug Blood Parasites Microbe Disease • Trypanosoma cruzi Chaga’s disease • Leishmania Leishmaniasis • Plasmodium vivax Malaria • Trypanosoma gambiense Sleeping sickness Leishmania Life Cycle Leishmania Vector: sandfly KM 1000x A Patient Suffering from leishmaniasis leishmaniasis DermAtlas, used with permission leishmaniasis DermAtlas, used with permission leishmaniasis DermAtlas, used with permission leishmaniasis Dermis.net, used with permission leishmaniasis Dermatology Image Atlas, used with permission Blood Parasites Microbe Disease • Trypanosoma cruzi Chaga’s disease • Leishmania Leishmaniasis • Plasmodium vivax Malaria • Trypanosoma gambiense Sleeping sickness Malaria • Causative agent: Protozoan Plasmodium • One of the most severe of all parasitic diseases • Endemic is most tropical areas • Plasmodium are amoeboid, intracellular protozoa that infect erythrocytes and other tissues • Transmitted by the female Anopheles mosquito The Worldwide Incidence of Malaria The life cycle of the malaria parasite Plasmodium Malaria in RBCs The “ring” stage of the malarial parasite Plasmodium falciparum Seen as dark, circular structures within red blood cells Plasmodium vivax Malaria KM 1000x Blood Parasites Microbe Disease • Trypanosoma cruzi Chaga’s disease • Leishmania Leishmaniasis • Plasmodium vivax Malaria • Trypanosoma gambiense Sleeping sickness Trypanosoma gambiense Sleeping Sickness KM Vector: Tsetse fly 1000x Blood Types Type A Type B Anti A Anti B antibody antibody Type AB Type O