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Things to Do Today
• Observe Urinalysis Plates
– Note hemolysis & Count Colonies on BAP
– Observe EMB plate
•
•
•
•
Blood Lecture
Blood Typing
Differential WBC Count
Observe Parasitic Blood Slides
Whole Blood
• Plasma
• Buffy coat
• Hematocrit
RBCs
RBC Disorders
• Anemias
-hemolytic
-Iron deficiency
-aplastic
-sickle cell
Polycythemia
WBCs
Wikipedia Commons, used with permission
WBCs
neutrophil
eosinophil
basophil
monocyte
Wiley
lymphocyte
WBCs
WebPath, used with permission
WBCs
lymphocyte
Medicine USC, used with permission
Vs.
monocyte
Leukocyte Disorders
• Leukemia
• Infectious mononucleosis
• Leukopenia
Formation of platelets
Clotting
Blood Types
Hemolytic DZ of the Newborn
• Mom must be Rh (-)
• Father must be Rh (+)
• A child must be Rh (+)
• A subsequent child must be Rh (+)
Hemolytic DZ of the Newborn
Blood typing
Watch demo of Blood Smear
10 sec in dye
20-24 sec in rinse
Blood Parasites
Microbe
Disease
• Trypanosoma cruzi
Chaga’s disease
• Leishmania
Leishmaniasis
• Plasmodium vivax
Malaria
• Trypanosoma gambiense
Sleeping sickness
Trypanosoma cruzi
1000x
Etiological Agent
for Chaga’s disease
KM
Rhodnius: Vector for Chaga’s
disease
KM
AKA assassin bug; kissing bug
Blood Parasites
Microbe
Disease
• Trypanosoma cruzi
Chaga’s disease
• Leishmania
Leishmaniasis
• Plasmodium vivax
Malaria
• Trypanosoma gambiense
Sleeping sickness
Leishmania Life Cycle
Leishmania
Vector: sandfly
KM
1000x
A Patient Suffering from leishmaniasis
leishmaniasis
DermAtlas, used with permission
leishmaniasis
DermAtlas, used with permission
leishmaniasis
DermAtlas, used with permission
leishmaniasis
Dermis.net, used
with permission
leishmaniasis
Dermatology Image Atlas, used
with permission
Blood Parasites
Microbe
Disease
• Trypanosoma cruzi
Chaga’s disease
• Leishmania
Leishmaniasis
• Plasmodium vivax
Malaria
• Trypanosoma gambiense
Sleeping sickness
Malaria
• Causative agent: Protozoan Plasmodium
• One of the most severe of all parasitic diseases
• Endemic is most tropical areas
• Plasmodium are amoeboid, intracellular
protozoa that infect erythrocytes and other
tissues
• Transmitted by the female Anopheles mosquito
The Worldwide Incidence of Malaria
The life cycle of the malaria parasite
Plasmodium
Malaria in RBCs
The “ring” stage of the
malarial parasite
Plasmodium falciparum
Seen as dark, circular
structures within red blood
cells
Plasmodium vivax
Malaria
KM
1000x
Blood Parasites
Microbe
Disease
• Trypanosoma cruzi
Chaga’s disease
• Leishmania
Leishmaniasis
• Plasmodium vivax
Malaria
• Trypanosoma gambiense
Sleeping sickness
Trypanosoma gambiense
Sleeping Sickness
KM
Vector: Tsetse fly
1000x
Blood Types
Type A
Type B
Anti A
Anti B
antibody
antibody
Type AB
Type O
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