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Isolation and characterization of a novel Dehalobacter
species strain TCP1 that reductively dechlorinates 2,4,6trichlorophenol
Shanquan Wang,† Weijie Zhang,‡ Kun-Lin Yang,‡ and Jianzhong He*,†
† Department
‡ Department
of Civil and Environmental Engineering
of Chemical and Biomolecular Engineering
National University of Singapore, Singapore 117576
Figure S1. DGGE characterization of dechlorinating bacteria present in culture TCP1 after
21 times of serial dilution. The PCR products were obtained by using the universal bacterial
primer set, 341FGC and 518R.
16S rRNA
Dehalobacter
Dehalobacter
Figure S2. Morphology of Dehalobacter sp. strain TCP1 as observed with fluorescence
microscopy. Cells were stained with fluorescent dye Syto-9 (5 µM).
5 µm
Figure S3. RpoB DGGE characterization of Dehalobacter bacteria present in culture
TCP1 (lane 1) and culture AD14-PCE (lane 2) with Dehalobacter genus-specific rpoB
gene targeted primers, DebrpoBFGC and DebrpoBR.
1
Dehalobacter sp. TCP1
Dehalobacter sp. AD14-PCE
2
Figure S4. Sequence heterogeneity of the five 16S rRNA genes in strain TCP1 when
excluding the insertion sequences (from base 255-1653).
16S-1 (JX999720)
16S-2 (JX999721)
16S-3 (JX999722)
16S-4 (JX999723)
16S-5 (JX999724)
16S-1 (JX999720)
16S-2 (JX999721)
16S-3 (JX999722)
16S-4 (JX999723)
16S-5 (JX999724)
Figure S5. Phylogenetic tree of identified putative chlorophenol reductive dehalogenase
gene - debcprA. Phylogenetic analyses are conducted in MEGA4 (Tamura et al. 2007).
debcprA (Dehalobacter sp. TCP1, JX999725)
putative rdhA (Dehalobacter sp. DCA, 13831363)
cprA (Desulfitobacterium chlororespirans, AAL84925)
pceA (Desulfitobacterium hafniense Y51, YP519072)
cprA (Desulfitobacterium sp. PCE-1, AF259790)
cprA (Desulfitobacterium hafniense DCB-2, AY013365)
cprA (Desulfitobacterium hafniense Y51, YP517388)