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Transcript 
Moving Green
Fluorescent Protein
•A jellyfish gene is identified
that codes for a protein (GFP)
that glows under UV light.
•Researchers clone the gene
and insert it into bacteria.
Plasmid from
E. coli
Gene that codes for
the inhibitor protein
Operator for the
arabinose
operon.
Gene that gives bacteria
resistance to the
antibiotic ampicillin.
Promoter for the
arabinose
operon.
Structural genes
for arabinose
metabolism
Function of the GFP gene in pGLO
Repressor protein
(from ara C gene)
O
P
Regulatory genes
(operator & promoter)
GREEN FLUOESCENT PROTEIN
GFP gene
(from jellyfish)
Function of the GFP gene in pGLO
RNA
polymerase
O
P
GREEN FLUOESCENT PROTEIN
Function of the GFP gene in pGLO
Arabinose
O
P
GREEN FLUOESCENT PROTEIN
Function of the GFP gene in pGLO
Arabinose
O
P
GREEN FLUOESCENT PROTEIN
Function of the GFP gene in pGLO
Arabinose
OArabinose
P
GREEN FLUOESCENT PROTEIN
Function of the GFP gene in pGLO
RNA
polymerase
Arabinose
O
P
GREEN FLUOESCENT PROTEIN
Function of the GFP gene in pGLO
RNA
polymerase
Arabinose
O
P
GREEN FLUOESCENT
mRNA
PROTEIN
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