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A typical bacterial transformation experiment.Here the plasmid pBR222 is the coning vector.(1)Cleavage of pBR222 with restriction enzyme BamHl,followed by(2) annealing and ligation of inserts generated by BamHl cleavage of some foreign DNA,(3)creates chimeric plasmid.(4)The chimeric plasmid is then used to transform Ca2+-treated heat-shocked E.coli cells,and the bacterial sample is plated on a petri plate.(5)following incubation of the petri plate overnight at 37℃ ,(6)colonies of ampr bacteria are evident.(7)Replica plating of these bacteria on plates of tetracycline-containing media(8)reveals which colonies are tetr and which are tetracycline sensitive (tet5).Only the tet5 colonies posses plasmids with foreign DNA inserts.
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