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BACTERIAL BASICS Bacteria Structure • Prokaryotes – No nucleus – Cell wall – DNA plasmid (ring-shaped) • Use asexual reproduction http://en.wikipedia.org/wiki/Bacteria Different Forms of DNA Transfer Bacterial Transformation • 1928-Frederick Griffith, 1944-Avery, McCarty, Macleod • Process of taking up DNA from media • DNA can be from cells from same or different species • DNA will integrate into the recipient’s chromosome http://en.wikipedia.org/wiki/Bacterial_transformation Bacterial Adhesion Zones • Adhesion zones develop in the bacteria cell membrane, although DNA cannot fit through Calcium Chloride Solution • The addition of calcium ions in CaCl2 solution shields the negatively charged phosphate groups so that the DNA can pass through the membrane when heat shocked. Heat Shock 42ºC
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