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Bacterial Growth on Public Restroom Toilet Seats vs. Bacterial Growth on Public Restroom Door Handles: A Microbiological Approach Jamie J. Larsen Undergraduate Student (Biology Concentration) Department of Biology Tennessee Technological University Cookeville, TN 38505 Project Summary Toilet Seats or Door Handles? Determine which surface contains the most bacteria Use cleaner on one of the restrooms tested to be used as a control Project Summary (cont’d) Obtain toilet seat and door handle samples in 10 public restrooms Public restroom toilet seats will contain the most bacterial growth Introduction People have come to desire information that would tell them how to disinfect particular areas (Ojima et al 2002) No one has yet used uniform sampling conditions to derive bacterial count distributions (Ojima et al 2002) Introduction (cont’d) The accumulation of this data would lead to research that could contribute to the estimation of infection risks for communicable diseases (Ojima et al 2002) Objective/Hypothesis Objective – Determine which surface contains the most bacteria Hypothesis – Public restroom toilet seats will contain the most bacteria Null Hypothesis – Both the toilet seats and the door handles will contain the same amount of bacteria Methods and Materials Materials (Goss 2006) – Data Table – 10 agar plates (divided in half) – Sterilized cotton swabs – Gloves – Distilled Water – Permanent Marker (to label plates) Methods and Materials (cont’d) Methods (Goss 2006) – Swab toilet seats and door handles in ten public restroom sites – Clean one site with bathroom cleaner – Label/Streak agar plates – Incubate for 48 hours – Check growth, do colony counts, and analyze results using the t-test statistic – Repeat experiment Expected Results and Benefits Expected Results – Samples taken from public restroom toilet seats will have the most bacterial growth on them – Samples taken from public restroom door handles will have less bacterial growth on them Benefits – Cleaner restrooms in the future – Raise awareness of possible infections obtained from public restroom surfaces Timeline Budget Thank You!! Thank you for your time and attention!!