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Prokaryotic Cell Structure and Function Size of Bacteria • Average bacteria 0.5 - 2.0 um in diam. – RBC is 7.5 um in diam. – Pulopiscium fishelsoni, bacillus (80µm in diameter and 200-600 µm long) – Thiomargarita namibiensis, sperical (100750 µm in diameter.) Shapes of Bacteria • • • • • • Coccus Bacillus Coccobacillus Vibrio Spirillum Spirochete Spherical Bacteria An average coccus is about 0.5-1.0 micrometer (µm) in diameter. • Streptococcus pneumoniae, A. Division in one plane. • Diplococcus: cocci arranged in pairs • Streptococcus: cocci arranged in chains • Streptococcus pyogenes, Spherical Bacteria B. Division in two planes. • tetrad: cocci arranged in squares of 4 C. Division in three planes • Sarcina: cocci in arranged cubes of 8 sarcina Spherical Bacteria D. Division in random planes • Staphylococcus: cocci arranged in irregular, often grape-like clusters Rod-Shaped Bacteria • Diplobacillus • Coccobacillus • Streptobacillus Spiral-Shaped Bacteria • Spirochete • Spirillum Cell Structures • Cell Membrane • Cytoplasm • Cell Wall • Components external to cell wall – Flagella – Pili – Capsule/ Slime layer Cell Membrane • Hydrophobic Proteins • Bilayer Phospholipid – Triclosan-enoyl transferase • Hopanoids Membrane proteins • Structural support • Detection of environmental signals – ToxR- Vibrio cholera • Secretion of virulence factor and communication signals – NodI and NodJ-Rhizobia Membrane lipids • Phosphoryl heads – Negative-phosphatidate • Phosphatidylglycerol • Cardiolipin – Positive-Phosphatidylethanolamine • DNA interaction • transporter protein • Fatty acids – Saturated straight chain (6-22) – Unsaturated • Oleic acid (cis) – Cyclopropane