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Western blotting of proteins isolated from bacteria and yeasts of the respiratory tract
(nose, pharyngeal swabs) of Cambodian (Km) and Kenyan (Ke) HIV positive children.
Used monoclonal antibodies against HIV-1 gp120 (1:750). lines 1-6 tested samples:
14Km, 17’Ke, 21Ke, 32’Km, 3’Km, 31Km, 14’Ke line 7: serum of AIDS patient
diluted 1:100; line 8: control bacteria; line 9: negative control bacteria Muta 104-0.
Internalization of HL-60 cells by bacteria of AIDS patients and
patients with colorectal tumors (GPA)
Patient/clone
P15/7
P1/4
P3/3
Mok12/5
Mok 1/6
K1-1
Number of bacterial colonies
2264
1340
1680
lysis of HL-60 cells
181
lysis of HL-60 cells
TuSG
71
883 S
104
negat. control
<5
-----------------------------------------------------------------------------------------Number of HL-60 cells 2x106/ml
Number of bact. cells 0,8x108
Internalization of normal human lymphocytes by bacteria of
AIDS patients (GPA)
Patient/clone
Number of bacterial colonies
P15/7
1121
P1/4
complete lyses of human lymphocytes
P3/3
complete lyses of human lymphocytes
Mok12/5
complete lysis of human lymphocytes
Mok 1/6
complete lysis of human lymphocytes
K1-1
complete lysis of human lymphocytes
Mok 22/5
423
725/5
1140
-----------------------------------------------------------------------------------------Number of lymphocytes 2x106/ml
Number of bact. cells 0,8x108
Calculation of probiotic treatment
(20 patients)
Viral load in time of probiotics treatment start:
808 600
Viral load in term of the end of probiotic treatment: 452 190
Reduction of viral load after probiotic treatment: 356 410
(56%)
Viral load of 11 patients from 20 decreased (60%)
The origin of HIV
According to our and other results there is strong
objection again generally accepted dogma that HIV was
transmitted to humans from apes in Africa about 35-50 or
according to last assertion 100 years ago en route of
accidental contacts.
There is not evidence about transmission of retroviruses
between two different species in nature. Should be HIV
only one exception in nature? Are there adequate proofs
for this statement?
The problem is that the acceptance of this dubious
argument has a major influence on – research, diagnosis
and therapy of AIDS.
How is it possible that this unconvincing argument was
adopted by a ​large part experts???
Our hypothesis
• HIV is of human origin and has been an integral part of humans from
the beginning of our existance. We inherited it from our ancestors.
Bacteria/yeasts are most likely natural host of HIV sequences.
• But, there is serious objection – if HIV was in our body from our
beginning, why did it’s emerge just about 25-35 years ago? To answer
this question we should go back into the ancient history of humankind.
In the past, major epidemics frequently occurred when there were new
patterns of transport between separately populated areas. This
tremendous longtime „sanitary“ process resulted in establishment of
the balance between these two kingdoms.
This balance was interrupted in the middle of the 20. century due to
acceptation of ATBs, drugs and changes in life style (homo and anal
sex). Propagated pathogenic microbes, some of which contain viral
and HIV genetic information, are provided resistance to antibiotics and
ability to penetrate into the body.
When bacteria carrying the HIV-like sequences penetrated into the
blood, interact with lymphocytes, infected/lyse them consequently the
immune system has collapsed. The result of this process is ..... AIDS
This hypothese is assumed that transmission of HIV from
apes to humans during last decades as a consequence of
their accidental contacts - is not a cause of AIDS.
The presence of HIV sequences in intestinal bacteria and
yeasts of the patients may be explained as follows:
1) Based on our results, we assume that intestinal bacteria
/ yeast is a natural host of HIV sequences in
extrachromosomal or other forms.
2) But it is conceivable that intestinal bacteria/yeast were
infected with the HIV genetic material, previously present
in human cells, particularly in macrophages / lymphocytes,
after their degradation.
• Our hypothesis may opens new opportunities for research
and treatment of AIDS
and now, we are able to
answer on many until now
unanswered questions
Presented hypothesis answer to many until now
unanswered questions:
- origin of HIV
- large scale HIV positivity in Africa,
- connection of AIDS with TBC in Africa,
- absence of „gold standard“ in Africa,
- the presence of HIV reservoirs after antiretroviral
therapy,
- atypical course of disease in comparision with
other retroviral infections
- the rarity of complete viral particles detection in
the material from AIDS patients, but detection of
HIV sequences and the HIV-like proteins (antigens)
herein
- high AZT toxicity
- and others.....