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Growth and Cell Division Growth Budding – Yeasts and some bacteria Binary fission – No mitosis – Unequal partitioning of cytoplasm – Multiple chromosomes 1 Bacterial Growth Curve Lag phase Log phase Stationary phase Death phase 2 Physical Factors Affecting Bacterial Growth pH Temperature – – – – – Psychrophiles Psychrotrophs Mesophiles Thermophiles Extreme thermophiles 3 Physical Factors Affecting Bacterial Growth - Oxygen Obligate aerobe Obligate anaerobe Microaerophile Facultative anaerobe Aerotolerant anaerobe 4 Physical Factors Affecting Bacterial Growth Hydrostatic pressure Osmotic pressure Radiation 5 Nutritional Factors Affecting Bacterial Growth Carbon source Nitrogen source Sulfur/ phosphorus Trace elements Vitamins Nutritional complexity 6 Culturing Bacteria - Streak Plates Streak plate method 7 Culturing Bacteria - Pour Plates Pour plate method 8 Culture Media Types – Defined Purposes Selective media Differential media Enrichment media Propagation media – Complex 9