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Using a Compound Light Microscope And Observing Live Protists how you found sizes of microorganisms • If the field diameter at 400 X is 450 µm, • How big is one of the following cells? • HINT: Divide 450 µm • how many across Human Skin Cells 400X Nucleus plasma membrane cytoplasm Onion Cells 400 X nucleus Cell wall Potato Cells 400 X • Dyed with iodine Starch crystals Elodea Cells • 100 X chloroplasts 400 X Elodea Cells • In fresh water • Full water vacuole In salt water empty water vacuole Cyclosis in Elodea • • • • Cyclosis in Elodea 1:09 Elodea cyclosis 1:56 Rapid cycling of cytoplasm You can see the chloroplasts moving also STENTOR • • • • • • Stentor youtube 2:36 Stentor Cilia 40 sec Horn-shaped Up to 2 mm long Cilia to bring in food Stretches Blespharisma • • • • • • Blespharisma Youtube 2:49 Blespharisma 2:18 150-300 µm long Ciliate Eat bacteria by drawing them in Often pink Paramecium • 50- 330 µm Slipper-shaped • Cilia, can spiral move • HD paramecium 13:06 • Paramecium eating dyed yeast (Congo Red) 1:59 Euglena • 25-100 µm • Flagellate • Euglena youtube 14:46 • Nice flagella movement 51 secs Presence of flagella (animal-like) Chlorophyll (plant-like) Red spot (light sensitive) Spirostomum • Spirostomum youtube 50 secs • HD Spirostomum 1:40 mouth 1-3 mm long Spiral rows of cilia Can be seen without a microscope